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Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma

  • Authors:
    • Chuanyu Ma
    • Jie Qin
    • Junpeng Zhang
    • Xingli Wang
    • Dongjun Wu
    • Xiunan Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116600, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 141-150
    |
    Published online on: November 11, 2019
       https://doi.org/10.3892/mmr.2019.10811
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Abstract

Increasing evidence has indicated that circular (circ)RNAs participate in carcinogenesis; however, the specific regulatory mechanisms underlying the effects of circRNAs, microRNAs (miRNAs/miRs) and genes on the development of clear cell renal cell carcinoma (CCRCC) remain unclear. In the present study, RNA microarray data from CCRCC tissues and control samples were downloaded from the Gene Expression Omnibus and The Cancer Genome Atlas, in order to identify significantly dysregulated circRNAs, miRNAs and genes. The Cancer‑Specific circRNA Database was used to explore the interactions between miRNAs and circRNAs, whereas TargetScan and miRDB were employed to predict the mRNA targets of miRNAs. Functional enrichment and prognostic analyses were conducted in R. The results revealed that 324 circRNAs were downregulated, whereas 218 circRNAs were upregulated in cancer. In addition, a circRNA‑miRNA‑mRNA interaction network was constructed. Gene Ontology analysis of the upregulated genes revealed that these genes were enriched in biological processes, including ‘flavonoid metabolic process’, ‘cellular glucuronidation’ and ‘T cell activation’. The downregulated genes were mainly enriched in biological processes, such as ‘nephron development’, ‘kidney development’ and ‘renal system development’. The hub genes, including membrane palmitoylated protein 7, aldehyde dehydrogenase 6 family member A1, transcription factor AP‑2α, collagen type IV α 4 chain, nuclear receptor subfamily 3 group C member 2, plasminogen, Holliday junction recognition protein, claudin 10, kinesin family member 18B and thyroid hormone receptor β, and the hub miRNAs, including miR‑21‑3p, miR‑155‑3p, miR‑144‑3p, miR‑142‑5p, miR‑875‑3p, miR‑885‑3p, miR‑3941, miR‑224‑3p, miR‑584‑3p and miR‑138‑1‑3p, were significantly associated with CCRCC survival. In conclusion, these results suggested that the significantly dysregulated circRNAs, miRNAs and genes identified in this study may be considered potential biomarkers of the carcinogenesis of CCRCC and the survival of patients with this disease.
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Copy and paste a formatted citation
Spandidos Publications style
Ma C, Qin J, Zhang J, Wang X, Wu D and Li X: Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma. Mol Med Rep 21: 141-150, 2020.
APA
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., & Li, X. (2020). Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma. Molecular Medicine Reports, 21, 141-150. https://doi.org/10.3892/mmr.2019.10811
MLA
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., Li, X."Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma". Molecular Medicine Reports 21.1 (2020): 141-150.
Chicago
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., Li, X."Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma". Molecular Medicine Reports 21, no. 1 (2020): 141-150. https://doi.org/10.3892/mmr.2019.10811
Copy and paste a formatted citation
x
Spandidos Publications style
Ma C, Qin J, Zhang J, Wang X, Wu D and Li X: Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma. Mol Med Rep 21: 141-150, 2020.
APA
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., & Li, X. (2020). Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma. Molecular Medicine Reports, 21, 141-150. https://doi.org/10.3892/mmr.2019.10811
MLA
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., Li, X."Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma". Molecular Medicine Reports 21.1 (2020): 141-150.
Chicago
Ma, C., Qin, J., Zhang, J., Wang, X., Wu, D., Li, X."Construction and analysis of circular RNA molecular regulatory networks in clear cell renal cell carcinoma". Molecular Medicine Reports 21, no. 1 (2020): 141-150. https://doi.org/10.3892/mmr.2019.10811
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