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Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells

  • Authors:
    • Sheng Zhou
    • Jing Yang
    • Mingwei Wang
    • Danying Zheng
    • Yizhi Liu
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat‑sen University, Guangzhou, Guangdong 510060, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 173-180
    |
    Published online on: November 12, 2019
       https://doi.org/10.3892/mmr.2019.10814
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Abstract

Epithelial‑to‑mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the role of ER stress on EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to disturb ER homeostasis, and 4‑phenylbutyric acid (PBA) or sodium tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed using ImageJ software. The results demonstrated that HLECs adopted an elongated morphology following treatment with TG, and the cellular aspect ratio increased. However, this morphological change was not observed following combination treatment with TG and PBA. Western blot analysis and immunofluorescence staining were used to measure the protein expression levels. A wound‑healing assay was performed to evaluate cell migration. Treatment with TM or TG increased the expression of the ER stress markers glucose‑regulated protein 78, phosphorylated eukaryotic initiation factor 2α, activating transcription factor (ATF)6, ATF4 and inositol‑requiring protein 1α and the EMT markers fibronectin, vimentin, α‑smooth muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased the expression of the epithelial cell marker epithelial cadherin and enhanced cell migration, which effects were inhibited following treatment with PBA or TUDCA. These results indicates that enhanced ER stress induced EMT and subsequently increased cell migration in HLECs in vitro.
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1 

Spalton D: Posterior capsule opacification: Have we made a difference? Br J Ophthalmol. 97:1–2. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Schaumberg DA, Dana MR, Christen WG and Glynn RJ: A systematic overview of the incidence of posterior capsule opacification. Ophthalmology. 105:1213–1221. 1998. View Article : Google Scholar : PubMed/NCBI

3 

Apple DJ, Escobar-Gomez M, Zaugg B, Kleinmann G and Borkenstein AF: Modern cataract surgery: Unfinished business and unanswered questions. Surv Ophthalmol. 56 (6 Suppl):S3–S53. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Mamuya FA, Wang Y, Roop VH, Scheiblin DA, Zajac JC and Duncan MK: The roles of alphaV integrins in lens EMT and posterior capsular opacification. J Cell Mol Med. 18:656–670. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Wormstone IM and Eldred JA: Experimental models for posterior capsule opacification research. Exp Eye Res. 142:2–12. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Zeisberg M and Neilson EG: Biomarkers for epithelial-mesenchymal transitions. J Clin Invest. 119:1429–1437. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

8 

van Roy F and Berx G: The cell-cell adhesion molecule E-cadherin. Cell Mol Life Sci. 65:3756–3788. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Takeichi M: Cadherin cell adhesion receptors as a morphogenetic regulator. Science. 251:1451–1455. 1991. View Article : Google Scholar : PubMed/NCBI

10 

Wijnhoven BP, Dinjens WN and Pignatelli M: E-cadherin-catenin cell-cell adhesion complex and human cancer. Br J Surg. 87:992–1005. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Lovicu FJ, Shin EH and McAvoy JW: Fibrosis in the lens. Sprouty regulation of TGFβ-signaling prevents lens EMT leading to cataract. Exp Eye Res. 142:92–101. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Erler P and Monaghan JR: The link between injury-induced stress and regenerative phenomena: A cellular and genetic synopsis. Biochim Biophys Acta. 1849:454–461. 2015. View Article : Google Scholar : PubMed/NCBI

13 

El-Hussuna A, Qvist N, Zangenberg MS, Langkilde A, Siersma V, Hjort S and Gögenur I: No effect of anti-TNF-a agents on the surgical stress response in patients with inflammatory bowel disease undergoing bowel resections: A prospective multi-center pilot study. BMC Surg. 18:912018. View Article : Google Scholar : PubMed/NCBI

14 

Nakka VP, Prakash-Babu P and Vemuganti R: Crosstalk between endoplasmic reticulum stress, oxidative stress, and autophagy: Potential therapeutic targets for acute CNS injuries. Mol Neurobiol. 53:532–544. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Shen X, Xue Y, Si Y, Wang Q, Wang Z, Yuan J and Zhang X: The unfolded protein response potentiates epithelial-to-mesenchymal transition (EMT) of gastric cancer cells under severe hypoxic conditions. Med Oncol. 32:4472015. View Article : Google Scholar : PubMed/NCBI

16 

Shin HS, Ryu ES, Oh ES and Kang DH: Endoplasmic reticulum stress as a novel target to ameliorate epithelial-to-mesenchymal transition and apoptosis of human peritoneal mesothelial cells. Lab Invest. 95:1157–1173. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Walter P and Ron D: The unfolded protein response: From stress pathway to homeostatic regulation. Science. 334:1081–1086. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Lindholm D, Korhonen L, Eriksson O and Kõks S: Recent Insights into the role of unfolded protein response in ER stress in health and disease. Front Cell Dev Biol. 5:482017. View Article : Google Scholar : PubMed/NCBI

19 

Yang J, Zhou S, Gu J, Wang Y, Guo M and Liu Y: Differences in unfolded protein response pathway activation in the lenses of three types of cataracts. PLoS One. 10:e01307052015. View Article : Google Scholar : PubMed/NCBI

20 

Puls TJ, Tan X, Whittington CF and Voytik-Harbin SL: 3D collagen fibrillar microstructure guides pancreatic cancer cell phenotype and serves as a critical design parameter for phenotypic models of EMT. PLoS One. 12:e01888702017. View Article : Google Scholar : PubMed/NCBI

21 

Cano A, Pérez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, Portillo F and Nieto MA: The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol. 2:76–83. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Zhong Q, Zhou B, Ann DK, Minoo P, Liu Y, Banfalvi A, Krishnaveni MS, Dubourd M, Demaio L, Willis BC, et al: Role of endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells: Effects of misfolded surfactant protein. Am J Respir Cell Mol Biol. 45:498–509. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Pallet N: New insights on stress-induced epithelial phenotypic changes. Nephrol Dial Transplant. 27:483–485. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Tanjore H, Cheng DS, Degryse AL, Zoz DF, Abdolrasulnia R, Lawson WE and Blackwell TS: Alveolar epithelial cells undergo epithelial-to-mesenchymal transition in response to endoplasmic reticulum stress. J Biol Chem. 286:30972–30980. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Lawson WE, Crossno PF, Polosukhin VV, Roldan J, Cheng DS, Lane KB, Blackwell TR, Xu C, Markin C, Ware LB, et al: Endoplasmic reticulum stress in alveolar epithelial cells is prominent in IPF: Association with altered surfactant protein processing and herpesvirus infection. Am J Physiol Lung Cell Mol Physiol. 294:L1119–L1126. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Shah PP, Dupre TV, Siskind LJ and Beverly LJ: Common cytotoxic chemotherapeutics induce epithelial-mesenchymal transition (EMT) downstream of ER stress. Oncotarget. 8:22625–22639. 2017. View Article : Google Scholar : PubMed/NCBI

27 

de Iongh RU, Wederell E, Lovicu FJ and McAvoy JW: Transforming growth factor-beta-induced epithelial-mesenchymal transition in the lens: A model for cataract formation. Cells Tissues Organs. 179:43–55. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Hales AM, Schulz MW, Chamberlain CG and McAvoy JW: TGF-beta 1 induces lens cells to accumulate alpha-smooth muscle actin, a marker for subcapsular cataracts. Curr Eye Res. 13:885–890. 1994. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou S, Yang J, Wang M, Zheng D and Liu Y: Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells. Mol Med Rep 21: 173-180, 2020.
APA
Zhou, S., Yang, J., Wang, M., Zheng, D., & Liu, Y. (2020). Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells. Molecular Medicine Reports, 21, 173-180. https://doi.org/10.3892/mmr.2019.10814
MLA
Zhou, S., Yang, J., Wang, M., Zheng, D., Liu, Y."Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells". Molecular Medicine Reports 21.1 (2020): 173-180.
Chicago
Zhou, S., Yang, J., Wang, M., Zheng, D., Liu, Y."Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells". Molecular Medicine Reports 21, no. 1 (2020): 173-180. https://doi.org/10.3892/mmr.2019.10814
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou S, Yang J, Wang M, Zheng D and Liu Y: Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells. Mol Med Rep 21: 173-180, 2020.
APA
Zhou, S., Yang, J., Wang, M., Zheng, D., & Liu, Y. (2020). Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells. Molecular Medicine Reports, 21, 173-180. https://doi.org/10.3892/mmr.2019.10814
MLA
Zhou, S., Yang, J., Wang, M., Zheng, D., Liu, Y."Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells". Molecular Medicine Reports 21.1 (2020): 173-180.
Chicago
Zhou, S., Yang, J., Wang, M., Zheng, D., Liu, Y."Endoplasmic reticulum stress regulates epithelial‑mesenchymal transition in human lens epithelial cells". Molecular Medicine Reports 21, no. 1 (2020): 173-180. https://doi.org/10.3892/mmr.2019.10814
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