Open Access

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

  • Authors:
    • Dong‑Gu Kim
    • Ji‑Won Choi
    • Il‑Joo Jo
    • Myoung‑Jin Kim
    • Ho‑Sub Lee
    • Seung‑Heon Hong
    • Ho‑Joon Song
    • Gi‑Sang Bae
    • Sung‑Joo Park
  • View Affiliations

  • Published online on: November 19, 2019     https://doi.org/10.3892/mmr.2019.10823
  • Pages: 258-266
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The major role of inner medullary collecting duct (IMCD) cells is to maintain water and sodium homeostasis. In addition to the major role, it also participates in the protection of renal and systemic inflammation. Although IMCD cells could take part in renal and systemic inflammation, investigations on renal inflammation in IMCD cells have rarely been reported. Although berberine (BBR) has been reported to show diverse pharmacological effects, its anti‑inflammatory and protective effects on IMCD cells have not been studied. Therefore, in the present study, we examined the anti‑inflammatory and protective effects of BBR in mouse IMCD‑3 (mIMCD‑3) cells against lipopolysaccharide (LPS). An MTT assay was carried out to investigate the toxicity of BBR on mIMCD‑3 cells. Reverse transcription quantitative‑PCR and western blotting were performed to analysis pro‑inflammatory molecules and cytokines. Mechanisms of BBR were examined by western blotting and immunocytochemistry. According to previous studies, pro‑inflammatory molecules, such as inducible nitric oxide synthase and cyclooxygenase‑2, and pro‑inflammatory cytokines, such as interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α are increased in LPS‑exposed mIMCD‑3 cells. However, the production of these pro‑inflammatory molecules is significantly inhibited by treatment with BBR. In addition, BBR inhibited translocation of nuclear factor (NF)‑κB p65 from the cytosol to the nucleus, and degradation of inhibitory κ‑Bα in LPS‑exposed mIMCD‑3 cells. In conclusion, BBR could inhibit renal inflammatory responses via inhibition of NF‑κB signaling and ultimately contribute to amelioration of renal injury during systemic inflammation.
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January-2020
Volume 21 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kim DG, Choi JW, Jo IJ, Kim MJ, Lee HS, Hong SH, Song HJ, Bae GS and Park SJ: Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway. Mol Med Rep 21: 258-266, 2020
APA
Kim, D., Choi, J., Jo, I., Kim, M., Lee, H., Hong, S. ... Park, S. (2020). Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway. Molecular Medicine Reports, 21, 258-266. https://doi.org/10.3892/mmr.2019.10823
MLA
Kim, D., Choi, J., Jo, I., Kim, M., Lee, H., Hong, S., Song, H., Bae, G., Park, S."Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway". Molecular Medicine Reports 21.1 (2020): 258-266.
Chicago
Kim, D., Choi, J., Jo, I., Kim, M., Lee, H., Hong, S., Song, H., Bae, G., Park, S."Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway". Molecular Medicine Reports 21, no. 1 (2020): 258-266. https://doi.org/10.3892/mmr.2019.10823