Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2020 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2020 Volume 21 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells

  • Authors:
    • Jun Sun
    • Yong Deng
    • Jin Shi
    • Wengang Yang
  • View Affiliations / Copyright

    Affiliations: Oncology Department, Jianhu Hospital Affiliated to Nantong University, Jianhu, Jiangsu 224700, P.R. China, Thoracic Surgery Department, Sheyang People's Hospital, Sheyang, Jiangsu 224300, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 35-42
    |
    Published online on: November 20, 2019
       https://doi.org/10.3892/mmr.2019.10836
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Dysregulation of microRNAs (miRNAs) is involved in the pathogenesis of esophageal cancer. miRNA (miR)‑542‑3p is a tumor suppressor in multiple types of cancer. However, whether and how miR‑542‑3p contributes to the progression of esophageal cancer remains unknown, and this is the aim of the present study. In the current study, decreased expression of miR‑542‑3p was detected in tumor tissues compared with normal tissues from patients with esophageal cancer, and miR‑542‑3p expression was negatively correlated with mRNA expression levels of ovarian tumor domain‑containing ubiquitin aldehyde‑binding protein 1 (OTUB1) in tumor tissues from patients with esophageal cancer. In KYSE150 human esophageal squamous cell carcinoma cells, overexpression of miR‑542‑3p significantly decreased OTUB1 at mRNA and protein levels, whereas downregulation of miR‑542‑3p significantly increased OTUB1 expression. Using a dual‑luciferase assay, OTUB1 was validated to be a target gene of miR‑542‑3p in KYSE150 cells. Functionally, miR‑542‑3p significantly inhibited the migration and invasion of KYSE150 cells by repression of OTUB1 expression. These results demonstrated that miR‑542‑3p may promote the metastasis of esophageal cancer cells, and indicated that miR‑542‑3p may be a treatment target for esophageal cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Rustgi AK and El-Serag HB: Esophageal carcinoma. N Engl J Med. 371:2499–2509. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Siegel R, Naishadham D and Jemal A: Cancer statistics for Hispanics/Latinos, 2012. CA Cancer J Clin. 62:283–298. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Deng HY, Alai G, Luo J, Li G, Zhuo ZG and Lin YD: Cancerous esophageal stenosis before treatment was significantly correlated to poor prognosis of patients with esophageal cancer: A meta-analysis. J Thorac Dis. 10:4212–4219. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Xu J, Chen Q, Liu P, Jia W, Chen Z and Xu Z: Integration of mRNA and miRNA analysis reveals the molecular mechanism underlying salt and alkali stress Tolerance in tobacco. Int J Mol Sci. 20:E23912019. View Article : Google Scholar : PubMed/NCBI

10 

Ye Y, Li SL and Wang SY: Construction and analysis of mRNA, miRNA, lncRNA, and TF regulatory networks reveal the key genes associated with prostate cancer. PLoS One. 13:e01980552018. View Article : Google Scholar : PubMed/NCBI

11 

Wang Y, Liang Y and Lu Q: MicroRNA epigenetic alterations: Predicting biomarkers and therapeutic targets in human diseases. Clin Genet. 74:307–315. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Feber A, Xi L, Luketich JD, Pennathur A, Landreneau RJ, Wu M, Swanson SJ, Godfrey TE and Litle VR: MicroRNA expression profiles of esophageal cancer. J Thorac Cardiovasc Surg. 135:255–260; discussion 260. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Yuan L, Yuan P, Yuan H, Wang Z, Run Z, Chen G, Zhao P and Xu B: miR-542-3p inhibits colorectal cancer cell proliferation, migration and invasion by targeting OTUB1. Am J Cancer Res. 7:159–172. 2017.PubMed/NCBI

14 

Althoff K, Lindner S, Odersky A, Mestdagh P, Beckers A, Karczewski S, Molenaar JJ, Bohrer A, Knauer S, Speleman F, et al: miR-542-3p exerts tumor suppressive functions in neuroblastoma by downregulating Survivin. Int J Cancer. 136:1308–1320. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Karunarathna U, Kongsema M, Zona S, Gong C, Cabrera E, Gomes AR, Man EP, Khongkow P, Tsang JW, Khoo US, et al: OTUB1 inhibits the ubiquitination and degradation of FOXM1 in breast cancer and epirubicin resistance. Oncogene. 35:1433–1444. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Baietti MF, Simicek M, Abbasi Asbagh L, Radaelli E, Lievens S, Crowther J, Steklov M, Aushev VN, Martínez García D, Tavernier J and Sablina AA: OTUB1 triggers lung cancer development by inhibiting RAS monoubiquitination. EMBO Mol Med. 8:288–303. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Wang Y, Zhou X, Xu M, Weng W, Zhang Q, Yang Y, Wei P and Du X: OTUB1-catalyzed deubiquitination of FOXM1 facilitates tumor progression and predicts a poor prognosis in ovarian cancer. Oncotarget. 7:36681–36697. 2016.PubMed/NCBI

18 

Zhou H, Liu Y, Zhu R, Ding F, Cao X, Lin D and Liu Z: OTUB1 promotes esophageal squamous cell carcinoma metastasis through modulating Snail stability. Oncogene. 37:3356–3368. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Agarwal V, Bell GW, Nam J and Bartel DP: Predicting effective microRNA target sites in mammalian mRNAs. Elife. Aug 4–2015.(Epub ahead of print). doi: 10.7554/eLife.05005. View Article : Google Scholar

21 

Lee K and Ferguson LR: MicroRNA biomarkers predicting risk, initiation and progression of colorectal cancer. World J Gastroenterol. 22:7389–7401. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Paliouras AR, Monteverde T and Garofalo M: Oncogene-induced regulation of microRNA expression: Implications for cancer initiation, progression and therapy. Cancer Lett. 421:152–160. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Fang Y, Fang D and Hu J: MicroRNA and its roles in esophageal cancer. Med Sci Monit. 18:RA22–RA30. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Li X, Wainscott C and Xi Y: MicroRNA provides insight into understanding esophageal cancer. Thorac Cancer. 2:134–142. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Qi B, Liu SG, Qin XG, Yao WJ, Lu JG, Guo L, Wang TY, Li HC and Zhao BS: Overregulation of microRNA-212 in the poor prognosis of esophageal cancer patients. Genet Mol Res. 13:7800–7807. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Wu W, Dang S, Feng Q, Liang J, Wang Y and Fan N: MicroRNA-542-3p inhibits the growth of hepatocellular carcinoma cells by targeting FZD7/Wnt signaling pathway. Biochem Biophys Res Commun. 482:100–105. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Wu Y, You J, Li F, Wang F and Wang Y: MicroRNA-542-3p suppresses tumor cell proliferation via targeting Smad2 inhuman osteosarcoma. Oncol Lett. 15:6895–6902. 2018.PubMed/NCBI

28 

Rang Z, Yang G, Wang YW and Cui F: miR-542-3p suppresses invasion and metastasis by targeting the proto-oncogene serine/threonine protein kinase, PIM1, in melanoma. Biochem Biophys Res Commun. 474:315–320. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Wiener R, DiBello AT, Lombardi PM, Guzzo CM, Zhang X, Matunis MJ and Wolberger C: E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Nat Struct Mol Biol. 20:1033–1039. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Sun XX, Challagundla KB and Dai MS: Positive regulation of p53 stability and activity by the deubiquitinating enzyme Otubain 1. EMBO J. 31:576–592. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Stanisić V, Malovannaya A, Qin J, Lonard DM and O'Malley BW: OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity. J Biol Chem. 284:16135–16145. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Peinado H, Olmeda D and Cano A: Snail, Zeb and bHLH factors in tumour progression: An alliance against the epithelial phenotype? Nat Rev Cancer. 7:415–428. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Mansoori B, Sandoghchian Shotorbani S and Baradaran B: RNA interference and its role in cancer therapy. Adv Pharm Bull. 4:313–321. 2014.PubMed/NCBI

34 

Hemmatzadeh M, Mohammadi H, Babaie F, Yousefi M, Ebrazeh M, Mansoori B, Shanehbandi D and Baradaran B: Snail-1 silencing by siRNA inhibits migration of TE-8 esophageal cancer cells through downregulation of metastasis-related genes. Adv Pharm Bull. 8:437–445. 2018. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Sun J, Deng Y, Shi J and Yang W: MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells. Mol Med Rep 21: 35-42, 2020.
APA
Sun, J., Deng, Y., Shi, J., & Yang, W. (2020). MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells. Molecular Medicine Reports, 21, 35-42. https://doi.org/10.3892/mmr.2019.10836
MLA
Sun, J., Deng, Y., Shi, J., Yang, W."MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells". Molecular Medicine Reports 21.1 (2020): 35-42.
Chicago
Sun, J., Deng, Y., Shi, J., Yang, W."MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells". Molecular Medicine Reports 21, no. 1 (2020): 35-42. https://doi.org/10.3892/mmr.2019.10836
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Deng Y, Shi J and Yang W: MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells. Mol Med Rep 21: 35-42, 2020.
APA
Sun, J., Deng, Y., Shi, J., & Yang, W. (2020). MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells. Molecular Medicine Reports, 21, 35-42. https://doi.org/10.3892/mmr.2019.10836
MLA
Sun, J., Deng, Y., Shi, J., Yang, W."MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells". Molecular Medicine Reports 21.1 (2020): 35-42.
Chicago
Sun, J., Deng, Y., Shi, J., Yang, W."MicroRNA‑542‑3p represses OTUB1 expression to inhibit migration and invasion of esophageal cancer cells". Molecular Medicine Reports 21, no. 1 (2020): 35-42. https://doi.org/10.3892/mmr.2019.10836
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team