Optimal conditions for the storage of German cockroach extract

Jeong,Kyoung  Yong; Lee,Jongsun; Yuk,Ji‑Eun; Park,Kyung  Hee; Lee,Jae‑Hyun; Kim,Jung  Dong; Park,Jung‑Won

doi:10.3892/mmr.2019.10854

February-2020 Volume 21 Issue 2

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February-2020 Volume 21 Issue 2

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Article

Optimal conditions for the storage of German cockroach extract

Authors:
- Kyoung Yong Jeong
- Jongsun Lee
- Ji‑Eun Yuk
- Kyung Hee Park
- Jae‑Hyun Lee
- Jung Dong Kim
- Jung‑Won Park
View Affiliations / Copyright

Affiliations: Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul 03722, Republic of Korea, Prolagen, Seoul 03722, Republic of Korea
Pages: 953-958
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Published online on: November 27, 2019

https://doi.org/10.3892/mmr.2019.10854
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Abstract

Allergen extracts are commonly utilized for diagnosis and immunotherapy; however, the stability of protease‑rich extracts is important for a precise diagnosis and treatment efficacy. The present study determines the optimal conditions for the storage of German cockroach allergen extract. Cockroach extracts were reconstituted in four buffers: normal saline (NS), 50% glycerol in NS, 0.3% phenol in NS, or 0.3% phenol and 50% glycerol in NS. The extracts in different buffers were stored either at room temperature (18‑26˚C, RT) or refrigerated (2‑8˚C). Subsequently, the protein concentration and allergen content (Bla g 1 and Bla g 2) in the extracts were examined for the course of one year. Extract potency was estimated by inhibition ELISA. At least 90.5% protein, 94.4% Bla g 1, 65.2% Bla g 2, and 91.4% potency remained after one year when 50% glycerol NS was added to the extract with refrigeration. However, less than 13.7% protein, 17.1% Bla g 1, 0% Bla g 2 and 32.5% potency were maintained after one year when 50% glycerol NS was not added to the extract and was maintained at RT. The addition of 0.3% phenol NS did not show significant effects on extract stability. The addition of 50% glycerol NS and refrigerated storage temperature were found to be important factors for increasing the shelf life of protease‑rich cockroach extract.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Optimal conditions for the storage of German cockroach extract

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