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Article

Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation

  • Authors:
    • Yingjie Xu
    • Meiyu Tan
    • Xiaoyu Tian
    • Jun Zhang
    • Jie Zhang
    • Jiajie Chen
    • Weihong Xu
    • Huiming Sheng
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Shanghai Tongren Hospital, Shanghai 200336, P.R. China, Department of Clinical Laboratory, Shanghai Tongren Hospital, Shanghai 200336, P.R. China, Digestive Disease Research Institute, Shanghai Huashan Hospital, Shanghai 200041, P.R. China
  • Pages: 945-952
    |
    Published online on: November 29, 2019
       https://doi.org/10.3892/mmr.2019.10855
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Abstract

Pancreatic cancer (PC) is the fourth leading cause of cancer‑related mortality worldwide. Leptin is an adipokine that is significantly increased in obese patients and that functions in various biological processes of cancer, such as tumor growth and metastasis. However, its role in PC cell proliferation and glucose metabolism and the underlying mechanisms remain unclear. In the present study, in vitro leptin treatment significantly promoted cell proliferation and increased glucose uptake and lactate production of human PC and healthy pancreas cells in a dose‑dependent manner, accompanied by increased expression of the glycolytic enzymes hexokinase II and glucose transporter 1. Furthermore, leptin receptor‑specific short hairpin RNAs were used to silence leptin receptor expression in PC cells, which had the opposite effect to leptin stimulation and decreased AKT phosphorylation. In addition, the effects of leptin stimulation were significantly counteracted by the AKT inhibitor LY294002, whereas the effects of leptin silencing were counteracted by AKT activator insulin‑like growth factor 1. The results of the present study suggested that leptin may contribute to cell proliferation and glucose metabolism of human PC cells, which may be through activation of the AKT pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xu Y, Tan M, Tian X, Zhang J, Zhang J, Chen J, Xu W and Sheng H: Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation. Mol Med Rep 21: 945-952, 2020.
APA
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J. ... Sheng, H. (2020). Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation. Molecular Medicine Reports, 21, 945-952. https://doi.org/10.3892/mmr.2019.10855
MLA
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J., Xu, W., Sheng, H."Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation". Molecular Medicine Reports 21.2 (2020): 945-952.
Chicago
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J., Xu, W., Sheng, H."Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation". Molecular Medicine Reports 21, no. 2 (2020): 945-952. https://doi.org/10.3892/mmr.2019.10855
Copy and paste a formatted citation
x
Spandidos Publications style
Xu Y, Tan M, Tian X, Zhang J, Zhang J, Chen J, Xu W and Sheng H: Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation. Mol Med Rep 21: 945-952, 2020.
APA
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J. ... Sheng, H. (2020). Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation. Molecular Medicine Reports, 21, 945-952. https://doi.org/10.3892/mmr.2019.10855
MLA
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J., Xu, W., Sheng, H."Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation". Molecular Medicine Reports 21.2 (2020): 945-952.
Chicago
Xu, Y., Tan, M., Tian, X., Zhang, J., Zhang, J., Chen, J., Xu, W., Sheng, H."Leptin receptor mediates the proliferation and glucose metabolism of pancreatic cancer cells via AKT pathway activation". Molecular Medicine Reports 21, no. 2 (2020): 945-952. https://doi.org/10.3892/mmr.2019.10855
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