Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2020 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2020 Volume 21 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Anti‑restriction protein KlcAHS enhances carbapenem resistance

  • Authors:
    • Wei Liang
    • Cheng Zhao
    • Yan Wang
    • Wenjun Zhu
    • Ying Zhang
    • Juan Hu
    • Xia Liu
    • Lin Yang
    • Wanchuan Zhuang
    • Yuping Shang
    • Guanhong Huang
    • Hui Shi
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, The Second People's Hospital of Lianyungang City, Lianyungang, Jiangsu 222023, P.R. China
  • Pages: 903-908
    |
    Published online on: December 16, 2019
       https://doi.org/10.3892/mmr.2019.10884
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The KlcAHS gene was previously identified as coexisting with the blaKPC‑2 gene in the backbone region of a series of blaKPC‑2‑harboring plasmids. The purpose of the present study was to determine the association between the KlcAHS and blaKPC‑2 genes. KlcAHS deletion and complementation experiments were used to evaluate the association between KlcAHS and carbapenem minimal inhibition concentrations (MICs). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis was used to detect changes in the expression levels of blaKPC‑2 upon knocking out the KlcAHS gene in a blaKPC‑2‑harboring plasmid. The imipenem MIC of the transformants harboring ΔKlcAHSpHS10842 was lower (16 µg/ml) than that of the transformants harboring wild‑type pHS10842 (32 µg/ml), whereas the kanamycin MIC of the transformants harboring pET24a was lower (1,024 µg/ml) than that of the transformants harboring pET24a‑KlcAHS (2,048 µg/ml). The imipenem MICs of the two NM1049 Escherichia coli strains carrying plasmids pHS092839 or ΔKlcAHSpHS092839 exceeded 16 µg/ml, whereas the ertapenem MIC of the host strains harboring ΔKlcAHSpHS092839 was 4 µg/ml compared with ≥8 µg/ml observed in the host strains carrying pHS092839. The RT‑qPCR results demonstrated that the messenger RNA expression levels of blaKPC‑2 in the transformants carrying ΔKlcAHSpHS092839 were significantly downregulated (P=0.007) compared with those in the transformants carrying pHS092839. These findings revealed that KlcAHS elevated the MIC values of various antibiotics by upregulating the expression levels of blaKPC‑2. Therefore, KlcAHS can confer increased resistance to carbapenems in host strains. The survival probability of clinical pathogens may be enhanced by the presence of the KlcAHS gene in antibiotics used on a large scale.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Tzouvelekis LS, Markogiannakis A, Psichogiou M, Tassios PT and Daikos GL: Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: An evolving crisis of global dimensions. Clin Microbiol Rev. 25:682–707. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Karaiskos I and Giamarellou H: Multidrug-resistant and extensively drug-resistant Gram-negative pathogens: Current and emerging therapeutic approaches. Expert Opin Pharmacother. 15:1351–1370. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Nordmann P, Cuzon G and Naas T: The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis. 9:228–236. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Tenover FC, Kalsi RK, Williams PP, Carey RB, Stocker S, Lonsway D, Rasheed JK, Biddle JW, McGowan JE Jr and Hanna B: Carbapenem resistance in Klebsiella pneumoniae not detected by automated susceptibility testing. Emerg Infect Dis. 12:1209–1213. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Anderson KF, Lonsway DR, Rasheed JK, Biddle J, Jensen B, McDougal LK, Carey RB, Thompson A, Stocker S, Limbago B and Patel JB: Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J Clin Microbiol. 45:2723–2725. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Naas T, Cuzon G, Truong HV and Nordmann P: Role of ISKpn7 and deletions in blaKPC gene expression. Antimicrob Agents Chemother. 56:4753–4759. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Kitchel B, Rasheed JK, Endimiani A, Hujer AM, Anderson KF, Bonomo RA and Patel JB: Genetic factors associated with elevated carbapenem resistance in KPC-producing Klebsiella pneumoniae. Antimicrob Agents Chemother. 54:4201–4207. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Pages JM, Peslier S, Keating TA, Lavigne JP and Nichols WW: Role of the outer membrane and porins in susceptibility of β-lactamase-producing enterobacteriaceae to ceftazidime--avibactam. Antimicrob Agents Chemother. 60:1349–1359. 2016. View Article : Google Scholar :

9 

Landman D, Bratu S and Quale J: Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae. J Med Microbiol. 58:1303–1308. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Lin XM, Yang JN, Peng XX and Li H: A novel negative regulation mechanism of bacterial outer membrane proteins in response to antibiotic resistance. J Proteome Res. 9:5952–5959. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Santajit S and Indrawattana N: Mechanisms of antimicrobial resistance in ESKAPE pathogens. Biomed Res Int. 2016:24750672016. View Article : Google Scholar : PubMed/NCBI

12 

Saw HT, Webber MA, Mushtaq S, Woodford N and Piddock LJ: Inactivation or inhibition of AcrAB-TolC increases resistance of carbapenemase-producing Enterobacteriaceae to carbapenems. J Antimicrob Chemother. 71:1510–1519. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Roth AL, Kurpiel PM, Lister PD and Hanson ND: bla(KPC) RNA expression correlates with two transcriptional start sites but not always with gene copy number in four genera of Gram-negative pathogens. Antimicrob Agents Chemother. 55:3936–3938. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Liang W, Xie Y, Xiong W, Tang Y, Li G, Jiang X and Lu Y: Anti-restriction protein, KlcAHS, promotes dissemination of carbapenem resistance. Front Cell Infect Microbiol. 7:1502017. View Article : Google Scholar : PubMed/NCBI

15 

Serfiotis-Mitsa D, Herbert AP, Roberts GA, Soares DC, White JH, Blakely GW, Uhrín D and Dryden DT: The structure of the KlcA and ArdB proteins reveals a novel fold and antirestriction activity against Type I DNA restriction systems in vivo but not in vitro. Nucleic Acids Res. 38:1723–1737. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Gust B, Challis GL, Fowler K, Kieser T and Chater KF: PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci USA. 100:1541–1546. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Zhang Y, Werling U and Edelmann W: Seamless Ligation Cloning Extract (SLiCE) cloning method. Methods Mol Biol. 1116:235–244. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Derbise A, Lesic B, Dacheux D, Ghigo JM and Carniel E: A rapid and simple method for inactivating chromosomal genes in Yersinia. FEMS Immunol Med Microbiol. 38:113–116. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Methods for antimicrobial susceptibility testing of anaerobic bacteria. 9th. CLSI standard M11. Clinical and Laboratory Standards Institute; Wayne, PA: 2018

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Marchaim D, Navon-Venezia S, Schwaber MJ and Carmeli Y: Isolation of imipenem-resistant Enterobacter species: Emergence of KPC-2 carbapenemase, molecular characterization, epidemiology, and outcomes. Antimicrob Agents Chemother. 52:1413–1418. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Elliott E, Brink AJ, van Greune J, Els Z, Woodford N, Turton J, Warner M and Livermore DM: In vivo development of ertapenem resistance in a patient with pneumonia caused by Klebsiella pneumoniae with an extended-spectrum beta-lactamase. Clin Infect Dis. 42:e95–e98. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Jacoby GA, Mills DM and Chow N: Role of beta-lactamases and porins in resistance to ertapenem and other beta-lactams in Klebsiella pneumoniae. Antimicrob Agents Chemother. 48:3203–3206. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Lartigue MF, Poirel L, Poyart C, Reglier-Poupet H and Nordmann P: Ertapenem resistance of Escherichia coli. Emerg Infect Dis. 13:315–317. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Szabo D, Silveira F, Hujer AM, Bonomo RA, Hujer KM, Marsh JW, Bethel CR, Doi Y, Deeley K and Paterson DL: Outer membrane protein changes and efflux pump expression together may confer resistance to ertapenem in Enterobacter cloacae. Antimicrob Agents Chemother. 50:2833–2835. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liang W, Zhao C, Wang Y, Zhu W, Zhang Y, Hu J, Liu X, Yang L, Zhuang W, Shang Y, Shang Y, et al: Anti‑restriction protein KlcAHS enhances carbapenem resistance. Mol Med Rep 21: 903-908, 2020.
APA
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J. ... Shi, H. (2020). Anti‑restriction protein KlcAHS enhances carbapenem resistance. Molecular Medicine Reports, 21, 903-908. https://doi.org/10.3892/mmr.2019.10884
MLA
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J., Liu, X., Yang, L., Zhuang, W., Shang, Y., Huang, G., Shi, H."Anti‑restriction protein KlcAHS enhances carbapenem resistance". Molecular Medicine Reports 21.2 (2020): 903-908.
Chicago
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J., Liu, X., Yang, L., Zhuang, W., Shang, Y., Huang, G., Shi, H."Anti‑restriction protein KlcAHS enhances carbapenem resistance". Molecular Medicine Reports 21, no. 2 (2020): 903-908. https://doi.org/10.3892/mmr.2019.10884
Copy and paste a formatted citation
x
Spandidos Publications style
Liang W, Zhao C, Wang Y, Zhu W, Zhang Y, Hu J, Liu X, Yang L, Zhuang W, Shang Y, Shang Y, et al: Anti‑restriction protein KlcAHS enhances carbapenem resistance. Mol Med Rep 21: 903-908, 2020.
APA
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J. ... Shi, H. (2020). Anti‑restriction protein KlcAHS enhances carbapenem resistance. Molecular Medicine Reports, 21, 903-908. https://doi.org/10.3892/mmr.2019.10884
MLA
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J., Liu, X., Yang, L., Zhuang, W., Shang, Y., Huang, G., Shi, H."Anti‑restriction protein KlcAHS enhances carbapenem resistance". Molecular Medicine Reports 21.2 (2020): 903-908.
Chicago
Liang, W., Zhao, C., Wang, Y., Zhu, W., Zhang, Y., Hu, J., Liu, X., Yang, L., Zhuang, W., Shang, Y., Huang, G., Shi, H."Anti‑restriction protein KlcAHS enhances carbapenem resistance". Molecular Medicine Reports 21, no. 2 (2020): 903-908. https://doi.org/10.3892/mmr.2019.10884
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team