Crocetin alleviates myocardial ischemia/reperfusion injury by regulating inflammation and the unfolded protein response

Yang,Ming; Mao,Genxiang; Ouyang,Lili; Shi,Chenhui; Hu,Pengfei; Huang,Shuwei

doi:10.3892/mmr.2019.10891

Crocetin alleviates myocardial ischemia/reperfusion injury by regulating inflammation and the unfolded protein response

Authors:
- Ming Yang
- Genxiang Mao
- Lili Ouyang
- Chenhui Shi
- Pengfei Hu
- Shuwei Huang
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Affiliations: The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Geriatrics, Zhejiang Provincial Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China, Department of Cardiology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310005, P.R. China
Published online on: December 17, 2019 https://doi.org/10.3892/mmr.2019.10891
Pages: 641-648
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Crocetin, a natural compound, has been demonstrated to exhibit beneficial effects in cardiovascular diseases. Previous studies demonstrated that crocetin reduced ischemia/reperfusion (I/R) injury by attenuating cytotoxicity and cellular apoptosis. However, the previous mechanistic studies did not fully elucidate its pharmacological effects on cardiac damage, especially I/R injury. The present study verified its cardioprotective effects in a Langendorff perfusion system, an ex vivo model of I/R. It was demonstrated that crocetin significantly attenuated the activities of pro‑inflammatory cytokines and nuclear factor erythroid‑2 related factor 2 (Nrf2)/heme oxygenase‑1 signaling. The present study provided novel insight that crocetin regulated the unfolded protein response (UPR) and decreased associated protein levels to protect the heart. Furthermore, it was identified that Nrf2 played a key role in the cardioprotective effect of crocetin by attenuating inflammation and the UPR.

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