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Article

microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1

  • Authors:
    • Lan Men
    • Dandan Nie
    • Haiying Nie
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Medicine, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China, Jilin Entry‑Exit Inspection and Quarantine Bureau, Changchun, Jilin 130062, P.R. China, Department of Vascular Surgery, China‑Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China
  • Pages: 1875-1882
    |
    Published online on: January 2, 2019
       https://doi.org/10.3892/mmr.2019.9804
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Abstract

An increasing number of studies have indicated that the dysregulation of microRNAs (miRNAs/miR) is closely associated with non‑small cell lung cancer (NSCLC) development and progression by acting as tumor suppressors or oncogenes. Therefore, an in‑depth understanding of the biological roles of miRNAs in NSCLC may provide novel therapeutic methods for the treatment of patients with this disease. In the present study, reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑577 expression in NSCLC tissues and cell lines. Cell Counting Kit‑8 and Transwell invasion assays were performed to determine the effects of miR‑577 on NSCLC cell proliferation and invasion. Luciferase reporter assays were used to demonstrate the relationship between miR‑577 and homeobox A1 (HOXA1) in NSCLC cells. The results revealed that miR‑577 was markedly downregulated in NSCLC tissues and cell lines. Additionally, restoration of miR‑577 expression significantly decreased the proliferation and invasion of NSCLC cells. Furthermore, miR‑577 negatively regulated HOXA1 expression in NSCLC cells by directly binding to its 3'‑untranslated region. HOXA1 was significantly upregulated in NSCLC tissues, and its upregulation was inversely correlated with miR‑577. Notably, restored HOXA1 expression abrogated the reduced proliferation and invasion of NSCLC cells caused by miR‑577 overexpression. Taken together, these results indicated that miR‑577 may have served tumor suppressive roles in NSCLC by directly targeting HOXA1. Therefore, this miRNA may be developed as a potential therapeutic target for the therapy of patients with NSCLC.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Laskin JJ and Sandler AB: State of the art in therapy for non-small cell lung cancer. Cancer Invest. 23:427–442. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Ramalingam SS, Owonikoko TK and Khuri FR: Lung cancer: New biological insights and recent therapeutic advances. CA Cancer J Clin. 61:91–112. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Collins LG, Haines C, Perkel R and Enck RE: Lung cancer: Diagnosis and management. Am Fam Physician. 75:56–63. 2007.PubMed/NCBI

5 

Chen W, Zheng R, Zhang S, Zhao P, Zeng H and Zou X: Report of cancer incidence and mortality in China, 2010. Ann Transl Med. 2:612014.PubMed/NCBI

6 

Kutikhin AG, Yuzhalin AE, Brailovskiy VV, Zhivotovskiy AS, Magarill YA and Brusina EB: Analysis of cancer incidence and mortality in the industrial region of South-East Siberia from 1991 through 2010. Asian Pac J Cancer Prev. 13:5189–5193. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Bradbury P, Sivajohanathan D, Chan A, Kulkarni S, Ung Y and Ellis PM: Postoperative adjuvant systemic therapy in completely resected non-small-cell lung cancer: A systematic review. Clin Lung Cancer. 18:259–273.e258. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Sandler A, Gray R, Perry MC, Brahmer J, Schiller JH, Dowlati A, Lilenbaum R and Johnson DH: Paclitaxel-carboplatin alone or with bevacizumab for non-small-cell lung cancer. N Engl J Med. 355:2542–2550. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Steeg PS: Metastasis suppressors alter the signal transduction of cancer cells. Nat Rev Cancer. 3:55–63. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Hydbring P and Badalian-Very G: Clinical applications of microRNAs. F1000Res. 2:1362013. View Article : Google Scholar : PubMed/NCBI

11 

Croce CM and Calin GA: miRNAs, cancer, and stem cell division. Cell. 122:6–7. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Castro D, Moreira M, Gouveia AM, Pozza DH and De Mello RA: MicroRNAs in lung cancer. Oncotarget. 8:81679–81685. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Yang CL, Zheng XL, Ye K, Ge H, Sun YN, Lu YF and Fan QX: MicroRNA-183 acts as a tumor suppressor in human non-small cell lung cancer by down-regulating MTA1. Cell Physiol Biochem. 46:93–106. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Yao Y, Shen H, Zhou Y, Yang Z and Hu T: MicroRNA-215 suppresses the proliferation, migration and invasion of non-small cell lung carcinoma cells through the downregulation of matrix metalloproteinase-16 expression. Exp Ther Med. 15:3239–3246. 2018.PubMed/NCBI

16 

Liu J, Jia Y, Jia L, Li T, Yang L and Zhang G: MicroRNA-615-3p inhibits the tumor growth and metastasis of NSCLC via inhibiting IGF2. Oncol Res. Mar 21–2018.(Epub ahead of print). doi: 10.3727/096504018X15215019227688.

17 

Li G, Wu F, Yang H, Deng X and Yuan Y: MiR-9-5p promotes cell growth and metastasis in non-small cell lung cancer through the repression of TGFBR2. Biomed Pharmacother. 96:1170–1178. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Wei K, Pan C, Yao G, Liu B, Ma T, Xia Y, Jiang W, Chen L and Chen Y: MiR-106b-5p promotes proliferation and inhibits apoptosis by regulating BTG3 in non-small cell lung cancer. Cell Physiol Biochem. 44:1545–1558. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Wang J, Lu Y, Ding H, Gu T, Gong C, Sun J, Zhang Z, Zhao Y and Ma C: The miR-875-5p inhibits SATB2 to promote the invasion of lung cancer cells. Gene. 644:13–19. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Li S, Gao M, Li Z, Song L, Gao X, Han J, Wang F, Chen Y, Li W, Yang J and Han X: Role of microRNAs in metastasis of non-small cell lung cancer. Front Biosci (Landmark Ed). 21:998–1005. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Yin C, Mou Q, Pan X, Zhang G, Li H and Sun Y: MiR-577 suppresses epithelial-mesenchymal transition and metastasis of breast cancer by targeting Rab25. Thorac Cancer. 9:472–479. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Wang LY, Li B, Jiang HH, Zhuang LW and Liu Y: Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting beta-catenin. Asian Pac J Trop Med. 8:923–929. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Jiang H, Ju H, Zhang L, Lu H and Jie K: microRNA-577 suppresses tumor growth and enhances chemosensitivity in colorectal cancer. J Biochem Mol Toxicol. 31:2017. View Article : Google Scholar :

24 

Xue KC, Hu DD, Zhao L, Li N and Shen HY: MiR-577 inhibits papillary thyroid carcinoma cell proliferation, migration and invasion by targeting SphK2. Eur Rev Med Pharmacol Sci. 21:3794–3800. 2017.PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Zhan B, Lu D, Luo P and Wang B: Prognostic value of expression of MicroRNAs in non-small cell lung cancer: A systematic review and meta-analysis. Clin Lab. 62:2203–2211. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Matikas A, Syrigos KN and Agelaki S: Circulating biomarkers in non-small-cell lung cancer: Current status and future challenges. Clin Lung Cancer. 17:507–516. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Ansari J, Shackelford RE and El-Ost H: Epigenetics in non-small cell lung cancer: From basics to therapeutics. Transl Lung Cancer Res. 5:155–171. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Ding X, Zhong T, Jiang L, Huang J, Xia Y and Hu R: miR-25 enhances cell migration and invasion in non-small-cell lung cancer cells via ERK signaling pathway by inhibiting KLF4. Mol Med Rep. 17:7005–7016. 2018.PubMed/NCBI

30 

Zhang W, Shen C, Li C, Yang G, Liu H, Chen X, Zhu D, Zou H, Zhen Y, Zhang D and Zhao S: miR-577 inhibits glioblastoma tumor growth via the Wnt signaling pathway. Mol Carcinog. 55:575–585. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Yu Z, Zhang W and Deng F: MicroRNA-577 inhibits gastric cancer growth by targeting E2F transcription factor 3. Oncol Lett. 10:1447–1452. 2015.PubMed/NCBI

32 

Yuan X, He J, Sun F and Gu J: Effects and interactions of MiR-577 and TSGA10 in regulating esophageal squamous cell carcinoma. Int J Clin Exp Pathol. 6:2651–2667. 2013.PubMed/NCBI

33 

Shah N and Sukumar S: The Hox genes and their roles in oncogenesis. Nat Rev Cancer. 10:361–371. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Grier DG, Thompson A, Kwasniewska A, McGonigle GJ, Halliday HL and Lappin TR: The pathophysiology of HOX genes and their role in cancer. J Pathol. 205:154–171. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Kusakabe M, Kutomi T, Watanabe K, Emoto N, Aki N, Kage H, Hamano E, Kitagawa H, Nagase T, Sano A, et al: Identification of G0S2 as a gene frequently methylated in squamous lung cancer by combination of in silico and experimental approaches. Int J Cancer. 126:1895–1902. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Zhang Y, Li XJ, He RQ, Wang X, Zhang TT, Qin Y, Zhang R, Deng Y, Wang HL, Luo DZ and Chen G: Upregulation of HOXA1 promotes tumorigenesis and development of nonsmall cell lung cancer: A comprehensive investigation based on reverse transcription-quantitative polymerase chain reaction and bioinformatics analysis. Int J Oncol. 53:73–86. 2018.PubMed/NCBI

37 

Zhan M, Qu Q, Wang G, Liu YZ, Tan SL, Lou XY, Yu J and Zhou HH: Let-7c inhibits NSCLC cell proliferation by targeting HOXA1. Asian Pac J Cancer Prev. 14:387–392. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Tian X, Ma J, Wang T, Tian J, Zhang Y, Mao L, Xu H and Wang S: Long non-coding RNA HOXA transcript antisense RNA myeloid-specific 1-HOXA1 axis downregulates the immunosuppressive activity of myeloid-derived suppressor cells in lung cancer. Front Immunol. 9:4732018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Men L, Nie D and Nie H: microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1. Mol Med Rep 19: 1875-1882, 2019.
APA
Men, L., Nie, D., & Nie, H. (2019). microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1. Molecular Medicine Reports, 19, 1875-1882. https://doi.org/10.3892/mmr.2019.9804
MLA
Men, L., Nie, D., Nie, H."microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1". Molecular Medicine Reports 19.3 (2019): 1875-1882.
Chicago
Men, L., Nie, D., Nie, H."microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1". Molecular Medicine Reports 19, no. 3 (2019): 1875-1882. https://doi.org/10.3892/mmr.2019.9804
Copy and paste a formatted citation
x
Spandidos Publications style
Men L, Nie D and Nie H: microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1. Mol Med Rep 19: 1875-1882, 2019.
APA
Men, L., Nie, D., & Nie, H. (2019). microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1. Molecular Medicine Reports, 19, 1875-1882. https://doi.org/10.3892/mmr.2019.9804
MLA
Men, L., Nie, D., Nie, H."microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1". Molecular Medicine Reports 19.3 (2019): 1875-1882.
Chicago
Men, L., Nie, D., Nie, H."microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1". Molecular Medicine Reports 19, no. 3 (2019): 1875-1882. https://doi.org/10.3892/mmr.2019.9804
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