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Article

Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition

  • Authors:
    • Jiayang Sun
    • Niwen Huang
    • Wen Ma
    • Haiyan Zhou
    • Kefang Lai
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550001, P.R. China, Department of Comprehensive Ward, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550001, P.R. China, Department of Clinical Research Centre, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550001, P.R. China, Department of Clinical Research State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 1817-1823
    |
    Published online on: January 2, 2019
       https://doi.org/10.3892/mmr.2019.9807
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Abstract

Asthma is a heterogeneous disease characterized by chronic airway inflammation. It has been demonstrated that metformin, an extensively used drug for the treatment of type 2 diabetes, improves airway inflammation and remodeling. However, the mechanism by which this occurs remains poorly understood. The present study investigated the protective effects of metformin in lipopolysaccharide (LPS)‑induced human bronchial epithelial (16HBE) cells injury and the associated mechanisms. 16HBE cells were preincubated with metformin for 1 h and subsequently exposed to LPS for 12 h. A lactate dehydrogenase (LDH) leakage assay was used to determine the extent of injury to 16HBE cells. The expression of tumor necrosis factor‑α (TNF‑α) and interleukin‑6 (IL‑6) was measured by ELISA. The protein expression of intercellular adhesion molecule‑1 (ICAM‑1) and vascular cell adhesion molecule‑1 (VCAM‑1), as well as proteins associated with nuclear factor (NF)‑κB signaling, was measured by western blotting. Immunofluorescence assays confirmed the nuclear translocation of NF‑κB p65. The LDH leakage assays suggested that metformin significantly reduced LPS‑induced 16HBE cell injury. Furthermore, it was confirmed that metformin suppressed the LPS‑induced secretion of TNF‑α, IL‑6, ICAM‑1 and VCAM‑1. The mechanism occurred at least partially via inhibition of NF‑κB signaling. The results demonstrated that metformin inhibited NF‑κB mRNA expression and the nuclear translocation of NF‑κB p65. To the best of our knowledge, the present study was the first to demonstrate that metformin ameliorated LPS‑induced bronchial epithelial cell injury via NF‑κB signaling suppression.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Huang N, Ma W, Zhou H and Lai K: Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition. Mol Med Rep 19: 1817-1823, 2019.
APA
Sun, J., Huang, N., Ma, W., Zhou, H., & Lai, K. (2019). Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition. Molecular Medicine Reports, 19, 1817-1823. https://doi.org/10.3892/mmr.2019.9807
MLA
Sun, J., Huang, N., Ma, W., Zhou, H., Lai, K."Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition". Molecular Medicine Reports 19.3 (2019): 1817-1823.
Chicago
Sun, J., Huang, N., Ma, W., Zhou, H., Lai, K."Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition". Molecular Medicine Reports 19, no. 3 (2019): 1817-1823. https://doi.org/10.3892/mmr.2019.9807
Copy and paste a formatted citation
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Spandidos Publications style
Sun J, Huang N, Ma W, Zhou H and Lai K: Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition. Mol Med Rep 19: 1817-1823, 2019.
APA
Sun, J., Huang, N., Ma, W., Zhou, H., & Lai, K. (2019). Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition. Molecular Medicine Reports, 19, 1817-1823. https://doi.org/10.3892/mmr.2019.9807
MLA
Sun, J., Huang, N., Ma, W., Zhou, H., Lai, K."Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition". Molecular Medicine Reports 19.3 (2019): 1817-1823.
Chicago
Sun, J., Huang, N., Ma, W., Zhou, H., Lai, K."Protective effects of metformin on lipopolysaccharide‑induced airway epithelial cell injury via NF‑κB signaling inhibition". Molecular Medicine Reports 19, no. 3 (2019): 1817-1823. https://doi.org/10.3892/mmr.2019.9807
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