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Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus

  • Authors:
    • Ji Hyeon Ahn
    • Bich Na Shin
    • Minah Song
    • Hyunjung Kim
    • Joon Ha Park
    • Tae‑Kyeong Lee
    • Cheol Woo Park
    • Young Eun Park
    • Jae‑Chul Lee
    • Jun‑Hwan Yong
    • Choong‑Hyun Lee
    • In Koo Hwang
    • Moo‑Ho Won
    • Yun Lyul Lee
  • View Affiliations / Copyright

    Affiliations: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea, Danchunok Company, Chuncheon, Gangwon 24210, Republic of Korea, Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Occupational Therapy, Dongnam Health University, Suwon, Gyeonggi 16238, Republic of Korea, Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam 31116, Republic of Korea, Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Physiology, College of Medicine and Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, Gangwon 24252, Republic of Korea
    Copyright: © Ahn et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1721-1727
    |
    Published online on: January 4, 2019
       https://doi.org/10.3892/mmr.2019.9822
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Abstract

Intermittent fasting (ImF) is known to reduce oxidative stress and affects adult neurogenesis in the hippocampal dentate gyrus. However, it is unknown how ImF affects endogenous antioxidants expressions, cell proliferation, and neuroblast differentiation and their dendrite remodeling over 3 months in the dentate gyrus of adult gerbils. The present study subjected 6‑month old male gerbils to a normal diet or alternate‑day ImF for 1, 2 and 3 months. Changes in body weight were not significantly different between gerbils fed a normal diet and on ImF. The present study also investigated the effects of ImF on antioxidant enzymes [superoxide dismutase (SOD)‑1, SOD2 and catalase] using immunohistochemistry, and endogenous cell proliferation, neuroblast differentiation and neuroblast dendrite complexity by using Ki67 (a cell proliferation marker) and doublecortin (neuroblast differentiation marker) immunohistochemistry in the dentate gyrus. SOD1, SOD2 and CAT immunoreactivities were shown in cells in the granule cell and polymorphic layers. SOD1, SOD2 and catalase immunoreactivity in the cells peaked at 2, 1 and 1 month, respectively, following ImF. Cell proliferation was ~250, 129 and 186% of the control, at 1, 2 and 3 months of ImF, respectively. Neuroblast differentiation was ~41, 32 and 12% of the control, at 1, 2 and 3 months of ImF, respectively, indicating that dendrites of neuroblasts were more arborized and developed at 3 months of ImF. Taken together, these results indicate that ImF for 3 months improves endogenous SOD1, SOD2 and catalase expressions and enhances cell proliferation, and neuroblast dendrites complexity and maturation in the adult gerbil dentate gyrus.
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Copy and paste a formatted citation
Spandidos Publications style
Ahn JH, Shin BN, Song M, Kim H, Park JH, Lee TK, Park CW, Park YE, Lee JC, Yong JH, Yong JH, et al: Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus. Mol Med Rep 19: 1721-1727, 2019.
APA
Ahn, J.H., Shin, B.N., Song, M., Kim, H., Park, J.H., Lee, T. ... Lee, Y. (2019). Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus. Molecular Medicine Reports, 19, 1721-1727. https://doi.org/10.3892/mmr.2019.9822
MLA
Ahn, J. H., Shin, B. N., Song, M., Kim, H., Park, J. H., Lee, T., Park, C. W., Park, Y. E., Lee, J., Yong, J., Lee, C., Hwang, I. K., Won, M., Lee, Y."Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus". Molecular Medicine Reports 19.3 (2019): 1721-1727.
Chicago
Ahn, J. H., Shin, B. N., Song, M., Kim, H., Park, J. H., Lee, T., Park, C. W., Park, Y. E., Lee, J., Yong, J., Lee, C., Hwang, I. K., Won, M., Lee, Y."Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus". Molecular Medicine Reports 19, no. 3 (2019): 1721-1727. https://doi.org/10.3892/mmr.2019.9822
Copy and paste a formatted citation
x
Spandidos Publications style
Ahn JH, Shin BN, Song M, Kim H, Park JH, Lee TK, Park CW, Park YE, Lee JC, Yong JH, Yong JH, et al: Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus. Mol Med Rep 19: 1721-1727, 2019.
APA
Ahn, J.H., Shin, B.N., Song, M., Kim, H., Park, J.H., Lee, T. ... Lee, Y. (2019). Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus. Molecular Medicine Reports, 19, 1721-1727. https://doi.org/10.3892/mmr.2019.9822
MLA
Ahn, J. H., Shin, B. N., Song, M., Kim, H., Park, J. H., Lee, T., Park, C. W., Park, Y. E., Lee, J., Yong, J., Lee, C., Hwang, I. K., Won, M., Lee, Y."Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus". Molecular Medicine Reports 19.3 (2019): 1721-1727.
Chicago
Ahn, J. H., Shin, B. N., Song, M., Kim, H., Park, J. H., Lee, T., Park, C. W., Park, Y. E., Lee, J., Yong, J., Lee, C., Hwang, I. K., Won, M., Lee, Y."Intermittent fasting increases the expressions of SODs and catalase in granule and polymorphic cells and enhances neuroblast dendrite complexity and maturation in the adult gerbil dentate gyrus". Molecular Medicine Reports 19, no. 3 (2019): 1721-1727. https://doi.org/10.3892/mmr.2019.9822
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