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Article

Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion

  • Authors:
    • Ru‑Jian Zhou
    • Hui‑Zeng Lv
  • View Affiliations / Copyright

    Affiliations: Department of Forensic Surgery, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510700, P.R. China
  • Pages: 2263-2270
    |
    Published online on: January 15, 2019
       https://doi.org/10.3892/mmr.2019.9856
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Abstract

Long noncoding RNAs (lncRNAs) are important regulators of various cellular and biological processes. The present study aimed to investigate the functions of a novel lncRNA, ACTA2‑AS1:4, a transcript variant of smooth muscle α‑actin 2‑antisense 1 (ACTA2‑AS1), in regulating liver cancer progression. Expression of lncRNAs in liver cancer tissues and cell lines were analyzed by reverse transcription quantitative polymerase chain reaction (RT‑qPCR). Knockdown of ACTA2‑AS1:4 expression in LM3 liver cancer cells was achieved by transfection with small interfering RNAs (siRNAs) that specifically targeted ACTA2‑AS1:4. The proliferation and cell cycle progression of ACTA2‑AS1:4‑silenced LM3 cells were determined using MTS assay and flow cytometry, respectively. A Transwell system assay was used to evaluate the migration and invasion capacities of LM3 cells transfected with ACTA2‑AS1:4 siRNA. The expression levels of major genes associated with important cellular processes were finally determined by RT‑qPCR and western blot analysis. ACTA2‑AS1:4 expression in liver cancer tissues and multiple cell lines was markedly downregulated by specific siRNAs. This inhibition of ACTA2‑AS1:4 expression significantly promoted the proliferation, cell cycle progression, migration and invasion of LM3 cells. A decrease in ACTA2‑AS1:4 expression also suppressed E‑cadherin expression, increased N‑cadherin expression, decreased caspase 3 expression and increased cyclin D1 and matrix metalloproteinase expression in liver cancer cells. Downregulation of ACTA2‑AS1:4 affects a number of key mechanisms involved in liver cancer progression. These data may be important for the future of liver cancer diagnosis and subsequent treatments.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou RJ and Lv HZ: Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion. Mol Med Rep 19: 2263-2270, 2019.
APA
Zhou, R., & Lv, H. (2019). Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion. Molecular Medicine Reports, 19, 2263-2270. https://doi.org/10.3892/mmr.2019.9856
MLA
Zhou, R., Lv, H."Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion". Molecular Medicine Reports 19.3 (2019): 2263-2270.
Chicago
Zhou, R., Lv, H."Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion". Molecular Medicine Reports 19, no. 3 (2019): 2263-2270. https://doi.org/10.3892/mmr.2019.9856
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou RJ and Lv HZ: Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion. Mol Med Rep 19: 2263-2270, 2019.
APA
Zhou, R., & Lv, H. (2019). Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion. Molecular Medicine Reports, 19, 2263-2270. https://doi.org/10.3892/mmr.2019.9856
MLA
Zhou, R., Lv, H."Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion". Molecular Medicine Reports 19.3 (2019): 2263-2270.
Chicago
Zhou, R., Lv, H."Knockdown of ACTA2‑AS1 promotes liver cancer cell proliferation, migration and invasion". Molecular Medicine Reports 19, no. 3 (2019): 2263-2270. https://doi.org/10.3892/mmr.2019.9856
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