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MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12

  • Authors:
    • Yankun Dai
    • Shanglun Liu
    • Xueguan Xie
    • Mingsheng Ding
    • Quan Zhou
    • Xiaoqing Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, P.R. China, Department of Orthopedics, Huai'an Hospital of Traditional Chinese Medicine, Huai'an, Jiangsu 223200, P.R. China
    Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2231-2237
    |
    Published online on: January 15, 2019
       https://doi.org/10.3892/mmr.2019.9859
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Abstract

The present study aimed to investigate the biological function and underlying molecular mechanisms of miR-31 in osteoarthritis (OA). Reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑31 expression, and it was found that miR‑31 was downregulated in the cartilage tissues of OA patients. microRNA.org was used to predict the gene targets of miR‑31, and dual luciferase reporter assays were used to verify that C‑X‑C motif chemokine ligand 12 (CXCL12) was a direct target of miR‑31. The human chondrocyte cell line CHON‑001 was used to perform MTT and cell migration assays. Western blotting was used to measure the protein expression of CXCL12, type I collagen and aggrecan. The results suggested that CXCL12 was a target of miR‑31, and the expression of CXCL12 was negatively regulated by miR‑31 in CHON‑001 cells. miR‑31 increased CHON‑001 cell viability and migration, as well as the expression of type I collagen and aggrecan. Furthermore, the overexpression of CXCL12 eliminated the effects of miR‑31 mimics on CHON‑001 cells. In conclusion, the data indicated that miR‑31 promoted chondrocyte viability and migration by directly targeting CXCL12, which provided evidence for CXCL12 as a potential target in OA therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Dai Y, Liu S, Xie X, Ding M, Zhou Q and Zhou X: MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12. Mol Med Rep 19: 2231-2237, 2019.
APA
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., & Zhou, X. (2019). MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12. Molecular Medicine Reports, 19, 2231-2237. https://doi.org/10.3892/mmr.2019.9859
MLA
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., Zhou, X."MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12". Molecular Medicine Reports 19.3 (2019): 2231-2237.
Chicago
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., Zhou, X."MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12". Molecular Medicine Reports 19, no. 3 (2019): 2231-2237. https://doi.org/10.3892/mmr.2019.9859
Copy and paste a formatted citation
x
Spandidos Publications style
Dai Y, Liu S, Xie X, Ding M, Zhou Q and Zhou X: MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12. Mol Med Rep 19: 2231-2237, 2019.
APA
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., & Zhou, X. (2019). MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12. Molecular Medicine Reports, 19, 2231-2237. https://doi.org/10.3892/mmr.2019.9859
MLA
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., Zhou, X."MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12". Molecular Medicine Reports 19.3 (2019): 2231-2237.
Chicago
Dai, Y., Liu, S., Xie, X., Ding, M., Zhou, Q., Zhou, X."MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine ligand 12". Molecular Medicine Reports 19, no. 3 (2019): 2231-2237. https://doi.org/10.3892/mmr.2019.9859
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