Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2019 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2019 Volume 19 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice

  • Authors:
    • Li Li
    • Xiaoyi Xu
    • Leilei Wu
    • Haicheng Zhu
    • Zhipeng He
    • Bo Zhang
    • Yanjun Chi
    • Gaochen Song
  • View Affiliations / Copyright

    Affiliations: Department of Basic Medicine, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Digestive Surgery, Mudanjiang Anorectal Hospital, Mudanjiang, Heilongjiang 157000, P.R. China, Department of Clinical Laboratory, Tumor Hospital of Mudanjiang City, Mudanjiang, Heilongjiang 157009, P.R. China, Department of Brain Surgery, Mudanjiang First People's Hospital, Mudanjiang, Heilongjiang 157011, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2254-2262
    |
    Published online on: January 15, 2019
       https://doi.org/10.3892/mmr.2019.9862
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to evaluate the antitumor effect of Scutellaria barbata polysaccharides (SBPS) in a hepatoma mouse model and examine the serum proteins involved in the tumorigenesis and SBPS treatment. A hepatoma model was established by the subcutaneous inoculation of murine hepatocellular carcinoma into Kunming mice. The treatment (once a day) lasted until the tumor weight in the model group was ~1 g (~7‑10 days after inoculation). The sera proteins from each group were then collected and subjected to two‑dimensional gel electrophoresis. Differentially expressed proteins were screened out and representatives were identified using matrix‑assisted laser desorption ionization time‑of‑flight mass spectrometry. SBPS treatment at different doses significantly inhibited hepatoma growth (all P<0.01 vs. model group). The comparative serum proteomics showed that pseudouridine synthase 1 and chain A of the signal recognition particle Alu RNA‑binding heterodimer (Srp9/14) were increased in the serum of the H22 hepatoma‑bearing mice, and both were reduced by SBPS treatment. Mitochondrial ribosomal protein L24 was absent from the serum of H22 hepatoma‑bearing mice, and was restored by SBPS treatment to approximately the normal level. Taken together, SBPS inhibited the growth of hepatic carcinoma in mice and affected serum proteomic profiling.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Yu J, Lei J, Yu H, Cai X and Zou G: Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata. Phytochemistry. 65:881–884. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Dai ZJ, Wang XJ, Li ZF, Ji ZZ, Ren HT, Tang W, Liu XX, Kang HF, Guan HT and Song LQ: Scutellaria barbate extract induces apoptosis of hepatoma H22 cells via the mitochondrial pathway involving caspase-3. World J Gastroenterol. 14:7321–7328. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Dai ZJ, Wu WY, Kang HF, Ma XB, Zhang SQ, Min WL, Kang WF, Ma XB, Zhang SQ, Min WL, et al: Protective effects of Scutellaria barbata against rat liver tumorigenesis. Asian Pac J Cancer Prev. 14:261–265. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Kan X, Zhang W, You R, Niu Y, Guo J and Xue J: Scutellaria barbata D. Don extract inhibits the tumor growth through down-regulating of Treg cells and manipulating Th1/Th17 immune response in hepatoma H22-bearing mice. BMC Complement Altern Med. 17:412017. View Article : Google Scholar : PubMed/NCBI

5 

Gao J, Lu WF, Dai ZJ, Lin S, Zhao Y, Li S, Zhao NN, Wang XJ, Kang HF, Ma XB and Zhang WG: Induction of apoptosis by total flavonoids from Scutellaria barbata D. Don in human hepatocarcinoma MHCC97-H cells via the mitochondrial pathway. Tumour Biol. 35:2549–2559. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Dai ZJ, Wang BF, Lu WF, Wang ZD, Ma XB, Min WL, Kang HF, Wang XJ and Wu WY: Total flavonoids of Scutellaria barbata inhibit invasion of hepatocarcinoma via MMP/TIMP in vitro. Molecules. 18:934–950. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Ye CL and Huang Q: Extraction of polysaccharides from herbal Scutellaria barbata D. Don (Ban-Zhi-Lian) and their antioxidant activity. Carbohydr Polym. 89:1131–1137. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Yang X, Yang Y, Tang S, Tang H, Yang G, Xu Q and Wu J: Anti-tumor effect of polysaccharides from Scutellaria barbata D. Don on the 95-D xenograft model via inhibition of the C-met pathway. J Pharmacol Sci. 125:255–263. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Yang J, Yang G, Hou G, Liu Q, Hu W, Zhao PU and He YI: Scutellaria barbata D. Don polysaccharides inhibit the growth of Calu-3 ×enograft tumors via suppression of the HER2 pathway and angiogenesis. Oncol Lett. 9:2721–2725. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Sun P, Sun D and Wang X: Effects of Scutellaria barbata polysaccharide on the proliferation, apoptosis and EMT of human colon cancer HT29 cells. Carbohydr Polym. 167:90–96. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Zhang AH, Sun H, Yan GL, Han Y and Wang XJ: Serum proteomics in biomedical research: A systematic review. Appl Biochem Biotechnol. 170:774–786. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Ng EW, Wong MY and Poon TC: Advances in MALDI mass spectrometry in clinical diagnostic applications. Top Curr Chem. 336:139–175. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Cao XL, Li H, Yu XL, Liang P, Dong BW, Fan J, Li M and Liu FY: Predicting early intrahepatic recurrence of hepatocellular carcinoma after microwave ablation using SELDI-TOF proteomic signature. PLoS One. 8:e824482013. View Article : Google Scholar : PubMed/NCBI

14 

Shen S, Peng H, Wang Y, Xu M, Lin M, Xie X, Peng B and Kuang M: Screening for immune-potentiating antigens from hepatocellular carcinoma patients after radiofrequency ablation by serum proteomic analysis. BMC Cancer. 18:1172018. View Article : Google Scholar : PubMed/NCBI

15 

Li H, Gu L, Zhong Y, Chen Y, Zhang L, Zhang AR, Sobol RW, Chen T and Li J: Administration of polysaccharide from Panax notoginseng prolonged the survival of H22 tumor-bearing mice. Onco Targets Ther. 9:3433–3441. 2016.PubMed/NCBI

16 

Song G, Wang G and Liu H: Experimental study of Scutellaria barbata polysaccharide on synergistic and attenuation effects of on cyclophosphamide. Tradit Chinese Med Inf. 27:107–109. 2010.(In Chinese).

17 

Song G, Yu Y and Wang X: Experiments on antitumor activity of Scutellaria barbata polysaccharides and its immunological mechanisms. World Sci Technol Mod Trad Chinese Med. 13:641–643. 2011.(In Chinese).

18 

Yu Y, Zhou Q and Yun K: Effects of Scutellaria barbata polysaccharide combined with adjuvant chemotherapy drugs on serum TNF-α and VEGF expression in mice. Tradit Chinese Med Inf. 27:29–31. 2010.(In Chinese).

19 

Xu X, Wu L, Li L, et al: Effects of Scutellaria barbata polysaccharide on tissue protein in H22 tumor bearing mice. J Chinese Gerontol. 36:4709–4710. 2016.(In Chinese).

20 

Hamma T and Ferre-D'Amare AR: Pseudouridine synthases. Chem Biol. 13:1125–1135. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Waalkes TP, Gehrke CW, Zumwalt RW, Chang SY, Lakings DB, Tormey DC, Ahmann DL and Moertel CG: The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer. Cancer. 36:390–398. 1975. View Article : Google Scholar : PubMed/NCBI

22 

Rasmuson T and Bjork GR: Urinary excretion of pseudouridine and prognosis of patients with malignant lymphoma. Acta Oncol. 34:61–67. 1995. View Article : Google Scholar : PubMed/NCBI

23 

Tu Z, Xu S and Wu M: Clinical value of urinary and serum pseudouridine in diagnosis and monitoring of primary liver cancer. Chin Med J (Engl). 108:204–208. 1995.PubMed/NCBI

24 

Chang DY, Hsu K and Maraia RJ: Monomeric scAlu and nascent dimeric Alu RNAs induced by adenovirus are assembled into SRP9/14-containing RNPs in HeLa cells. Nucleic Acids Res. 24:4165–4170. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Berger A, Ivanova E, Gareau C, Scherrer A, Mazroui R and Strub K: Direct binding of the Alu binding protein dimer SRP9/14 to 40S ribosomal subunits promotes stress granule formation and is regulated by Alu RNA. Nucleic Acids Res. 42:11203–11217. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Rho JH, Qin S, Wang JY and Roehrl MH: Proteomic expression analysis of surgical human colorectal cancer tissues: UP-regulation of PSB7, PRDX1, and SRP9 and hypoxic adaptation in cancer. J Proteome Res. 7:2959–2972. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Liu Y, Zhu X, Zhu J, Liao S, Tang Q, Liu K, Guan X, Zhang J and Feng Z: Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray. Oncol Rep. 18:943–951. 2007.PubMed/NCBI

28 

Sylvester JE, Fischel-Ghodsian N, Mougey EB and O'Brien TW: Mitochondrial ribosomal proteins: Candidate genes for mitochondrial disease. Genet Med. 6:73–80. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Amunts A, Brown A, Toots J, Scheres SHW and Ramakrishnan V: Ribosome. The structure of the human mitochondrial ribosome. Science. 348:95–98. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li L, Xu X, Wu L, Zhu H, He Z, Zhang B, Chi Y and Song G: Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice. Mol Med Rep 19: 2254-2262, 2019.
APA
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B. ... Song, G. (2019). Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice. Molecular Medicine Reports, 19, 2254-2262. https://doi.org/10.3892/mmr.2019.9862
MLA
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B., Chi, Y., Song, G."Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice". Molecular Medicine Reports 19.3 (2019): 2254-2262.
Chicago
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B., Chi, Y., Song, G."Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice". Molecular Medicine Reports 19, no. 3 (2019): 2254-2262. https://doi.org/10.3892/mmr.2019.9862
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Xu X, Wu L, Zhu H, He Z, Zhang B, Chi Y and Song G: Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice. Mol Med Rep 19: 2254-2262, 2019.
APA
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B. ... Song, G. (2019). Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice. Molecular Medicine Reports, 19, 2254-2262. https://doi.org/10.3892/mmr.2019.9862
MLA
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B., Chi, Y., Song, G."Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice". Molecular Medicine Reports 19.3 (2019): 2254-2262.
Chicago
Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B., Chi, Y., Song, G."Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22‑bearing mice". Molecular Medicine Reports 19, no. 3 (2019): 2254-2262. https://doi.org/10.3892/mmr.2019.9862
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team