Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

Sun,Zhihua; Liu,Laizhen; Zhang,Hui; Li,Youwen; Wei,Feng; Li,Zhiqiang; Wang,Pengyan; Fu,Qiang; Ren,Yan; Zhang,Yu; Guo,Zhiru; Chen,Chuangfu

doi:10.3892/mmr.2019.9868

March-2019 Volume 19 Issue 3

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March-2019 Volume 19 Issue 3

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Article

Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

Authors:
- Zhihua Sun
- Laizhen Liu
- Hui Zhang
- Youwen Li
- Feng Wei
- Zhiqiang Li
- Pengyan Wang
- Qiang Fu
- Yan Ren
- Yu Zhang
- Zhiru Guo
- Chuangfu Chen
View Affiliations / Copyright

Affiliations: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832000, P.R. China, College of Biology and Food, Shangqiu Normal University, Shangqiu, Henan 476000, P.R. China, College of Medicine, Shihezi University, Shihezi, Xinjiang 832000, P.R. China
Pages: 2323-2329
|
Published online on: January 16, 2019

https://doi.org/10.3892/mmr.2019.9868
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Abstract

The Capripoxvirus (CaPV) has a large double‑stranded DNA genome and a restricted host‑range. At present, it is being investigated as an ideal vaccine vector. In the present study, a novel recombinant goat pox virus (rGTPV) was constructed to express Brucella outer membrane protein (OMP)25, and was validated by in vitro and in vivo immunization assays. A novel rGTPV vector was created, in which the thymidine kinase gene was used as a flanking sequence, I1L was inserted as a promoter element to enhance Brucella OMP25 expression, and p7.5 as another promoter element was used to regulate guanine phosphoribosyl‑transferase as a selection maker. The rGTPV vector was transfected into sheep fetal fibroblast/lamb testis cells pre‑infected with GTPV G14‑STV44‑55 to recombine. Brucella OMP25 protein was expressed in cells by rGTPV, and activated immune reactivity to Brucella OMP25 protein, as detected by western blotting. Furthermore, rGTPV elicited, anti‑Brucella‑specific immunoglobulin G responses, as determined by ELISA. Mice vaccinated with rGTPV did not exhibit pathology alterations in the kidney and liver. These results suggested that the novel rGTPV was able to efﬁciently drive Brucella OMP25 protein expression and activate immune reactivity, and may have applications in CaPV live vector vaccines and associated research.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression and functional analysis of Brucella outer membrane protein 25 in recombinant goat pox virus

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