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Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase

  • Authors:
    • Yong‑Yu Yang
    • Ling‑Xing Shi
    • Jian‑He Li
    • Liang‑Yuan Yao
    • Da‑Xiong Xiang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Pharmacology, Changsha Medical University, Changsha, Hunan 410219, P.R. China, Hunan Provincial Engineering Research Center of Translational Medical and Innovative Drug, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2245-2253
    |
    Published online on: January 17, 2019
       https://doi.org/10.3892/mmr.2019.9875
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Abstract

Diabetic nephropathy (DN) is among the most common complications of diabetes mellitus. The disorder is associated with a decrease in the activity of the nitric oxide synthase/nitric oxide system. Piperazine ferulate (PF) is widely used for the treatment of kidney disease in China. The aim of the present study was to examine the effects of PF on streptozotocin (STZ)‑induced DN and the underlying mechanism of this process. STZ‑induced diabetic mice were intragastrically administered PF (100, 200 and 400 mg/kg/body weight/day) for 12 weeks. At the end of the treatment period, the parameters of 24‑h albuminuria and blood urea nitrogen, creatinine and oxidative stress levels were measured. Hematoxylin and eosin staining, periodic acid‑Schiff staining and electron microscopy were used to evaluate the histopathological alterations. mRNA and protein expression of endothelial nitric oxide synthase (eNOS) were measured by quantitative polymerase chain reaction and western blotting, respectively. PF significantly decreased blood urea nitrogen and creatinine levels and 24‑h albuminuria, and it alleviated oxidative stress, improved glomerular basement membrane thickness and caused an upregulation in eNOS expression and activity levels in diabetic mice. In addition, high glucose decreased eNOS expression levels, whereas PF caused a reversal in the nitric oxide (NO) levels of glomerular endothelial cells. The present results suggested that PF exhibited renoprotective effects on DN. The mechanism of its action was associated with the regulation of eNOS expression and activity.
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Copy and paste a formatted citation
Spandidos Publications style
Yang YY, Shi LX, Li JH, Yao LY and Xiang DX: Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase. Mol Med Rep 19: 2245-2253, 2019.
APA
Yang, Y., Shi, L., Li, J., Yao, L., & Xiang, D. (2019). Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase. Molecular Medicine Reports, 19, 2245-2253. https://doi.org/10.3892/mmr.2019.9875
MLA
Yang, Y., Shi, L., Li, J., Yao, L., Xiang, D."Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 19.3 (2019): 2245-2253.
Chicago
Yang, Y., Shi, L., Li, J., Yao, L., Xiang, D."Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 19, no. 3 (2019): 2245-2253. https://doi.org/10.3892/mmr.2019.9875
Copy and paste a formatted citation
x
Spandidos Publications style
Yang YY, Shi LX, Li JH, Yao LY and Xiang DX: Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase. Mol Med Rep 19: 2245-2253, 2019.
APA
Yang, Y., Shi, L., Li, J., Yao, L., & Xiang, D. (2019). Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase. Molecular Medicine Reports, 19, 2245-2253. https://doi.org/10.3892/mmr.2019.9875
MLA
Yang, Y., Shi, L., Li, J., Yao, L., Xiang, D."Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 19.3 (2019): 2245-2253.
Chicago
Yang, Y., Shi, L., Li, J., Yao, L., Xiang, D."Piperazine ferulate ameliorates the development of diabetic nephropathy by regulating endothelial nitric oxide synthase". Molecular Medicine Reports 19, no. 3 (2019): 2245-2253. https://doi.org/10.3892/mmr.2019.9875
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