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Article

Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway

  • Authors:
    • Mingyu Song
    • Fafa Tian
    • Huang Xia
    • Yuanyuan Xie
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 3255-3262
    |
    Published online on: February 14, 2019
       https://doi.org/10.3892/mmr.2019.9951
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Abstract

Repulsive guidance molecule a (RGMa) is a membrane‑associated glycoprotein that regulates axonal guidance and inhibits axon outgrowth. In our previous study, we hypothesized that RGMa may be involved in temporal lobe epilepsy (TLE) via the repulsive guidance molecule a (RGMa)‑focal adhesion kinase (FAK)‑Ras signaling pathway. To investigate the role of RGMa in epilepsy, recombinant RGMa protein and FAK inhibitor 14 was intracerebroventricularly injected into a pentylenetetrazol (PTZ) kindling model and Timm staining, co‑immunoprecipitation and western blotting analyses were subsequently performed. The results of the present study revealed that intracerebroventricular injection of recombinant RGMa protein reduced the phosphorylation of FAK (Tyr397) and intracerebroventricular injection of FAK inhibitor 14 reduced the interaction between FAK and p120GAP, as wells as Ras expression. Recombinant RGMa protein and FAK inhibitor 14 exerted seizure‑suppressant effects; however, recombinant RGMa protein but not FAK inhibitor 14 suppressed mossy fiber sprouting in the PTZ kindling model. Collectively, these results demonstrated that RGMa may be considered as a potential therapeutic agent for epilepsy, and that RGMa may exert the aforementioned biological effects partly via the FAK‑p120GAP‑Ras signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Song M, Tian F, Xia H and Xie Y: Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway. Mol Med Rep 19: 3255-3262, 2019.
APA
Song, M., Tian, F., Xia, H., & Xie, Y. (2019). Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway. Molecular Medicine Reports, 19, 3255-3262. https://doi.org/10.3892/mmr.2019.9951
MLA
Song, M., Tian, F., Xia, H., Xie, Y."Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway". Molecular Medicine Reports 19.4 (2019): 3255-3262.
Chicago
Song, M., Tian, F., Xia, H., Xie, Y."Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway". Molecular Medicine Reports 19, no. 4 (2019): 3255-3262. https://doi.org/10.3892/mmr.2019.9951
Copy and paste a formatted citation
x
Spandidos Publications style
Song M, Tian F, Xia H and Xie Y: Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway. Mol Med Rep 19: 3255-3262, 2019.
APA
Song, M., Tian, F., Xia, H., & Xie, Y. (2019). Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway. Molecular Medicine Reports, 19, 3255-3262. https://doi.org/10.3892/mmr.2019.9951
MLA
Song, M., Tian, F., Xia, H., Xie, Y."Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway". Molecular Medicine Reports 19.4 (2019): 3255-3262.
Chicago
Song, M., Tian, F., Xia, H., Xie, Y."Repulsive guidance molecule a suppresses seizures and mossy fiber sprouting via the FAK‑p120RasGAP‑Ras signaling pathway". Molecular Medicine Reports 19, no. 4 (2019): 3255-3262. https://doi.org/10.3892/mmr.2019.9951
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