Open Access

Combining antioxidant astaxantin and cholinesterase inhibitor huperzine A boosts neuroprotection

  • Authors:
    • Xin Yang
    • Han‑Mei Wei
    • Guo‑Yan Hu
    • Jun Zhao
    • Li‑Na Long
    • Chang‑Jian Li
    • Zi‑Jun Zhao
    • He‑Kun Zeng
    • Hong Nie
  • View Affiliations

  • Published online on: January 9, 2020     https://doi.org/10.3892/mmr.2020.10920
  • Pages: 1043-1050
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxidative stress is a pathophysiological condition resulting in neurotoxicity, which is possibly associated with neurodegenerative disorders. In this study, the antioxidative effects of the antioxidant astaxanthin (AXT) in combination with huperzine A (HupA), which is used as a cholinesterase inhibitor for the treatment of Alzheimer's disease, were investigated. PC12 cells were treated with either tert‑butyl hydroperoxide (TBHP), or with the toxic version of β‑amyloid, Aβ25‑35, to induce oxidative stress and neurotoxicity. Cell viability, morphology, lactate dehydrogenase (LDH) release, intracellular accumulation of reactive oxygen species (ROS), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were determined, while neuroprotection was also monitored using an MTT assay. It was found that combining AXT with HupA significantly increased the viability of PC12 cells, prevented membrane damage (as measured by LDH release), attenuated intracellular ROS formation, increased SOD activity and decreased the level of MDA after TBHP exposure when compared to these drugs administered alone. Pretreatment with HupA and AXT decreased toxic damage produced by Aβ25‑35. These data indicated that combining an antioxidant with a cholinesterase inhibitor increases the degree of neuroprotection; with future investigation this could be a potential therapy used to decrease neurotoxicity in the brain.
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March 2020
Volume 21 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Yang, X., Wei, H., Hu, G., Zhao, J., Long, L., Li, C. ... Nie, H. (2020). Combining antioxidant astaxantin and cholinesterase inhibitor huperzine A boosts neuroprotection. Molecular Medicine Reports, 21, 1043-1050. https://doi.org/10.3892/mmr.2020.10920
MLA
Yang, X., Wei, H., Hu, G., Zhao, J., Long, L., Li, C., Zhao, Z., Zeng, H., Nie, H."Combining antioxidant astaxantin and cholinesterase inhibitor huperzine A boosts neuroprotection". Molecular Medicine Reports 21.3 (2020): 1043-1050.
Chicago
Yang, X., Wei, H., Hu, G., Zhao, J., Long, L., Li, C., Zhao, Z., Zeng, H., Nie, H."Combining antioxidant astaxantin and cholinesterase inhibitor huperzine A boosts neuroprotection". Molecular Medicine Reports 21, no. 3 (2020): 1043-1050. https://doi.org/10.3892/mmr.2020.10920