Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation

Nakazawa,Yosuke; Aoki,Miki; Ishiwa,Sho; Morishita,Naoki; Endo,Shin; Nagai,Noriaki; Yamamoto,Naoki; Funakoshi‑Tago,Megumi; Tamura,Hiroomi

doi:10.3892/mmr.2020.10941

Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation

Authors:
- Yosuke Nakazawa
- Miki Aoki
- Sho Ishiwa
- Naoki Morishita
- Shin Endo
- Noriaki Nagai
- Naoki Yamamoto
- Megumi Funakoshi‑Tago
- Hiroomi Tamura
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Affiliations: Department of Hygienic Chemistry, Faculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan, R&D Division, Hayashibara Co., Ltd, Okayama 702‑8006, Japan, Department of Advanced Design for Pharmaceuticals, Faculty of Pharmacy, Kindai University, Osaka 577‑8502, Japan, Regenerative Medicine Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, Toyoake, Aichi 470‑1192, Japan
Published online on: January 14, 2020 https://doi.org/10.3892/mmr.2020.10941
Pages: 1258-1266
Copyright: © Nakazawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti‑cataract effects of α‑glucosyl hesperidin in vivo and in vitro using a selenite‑induced cataract model. SD rats (age, 13 days) were orally administered PBS (0.2 ml) or α‑glucosyl hesperidin (200 mg/kg) on days 0, 1 and 2. Sodium selenite was subcutaneously administered to the rats 4 h after the first oral administration on day 0. Antioxidant levels in the lens and blood were measured on day 6. In vitro, human lens epithelial cells were treated with sodium selenite (10 µM) and/or hesperetin (50 or 100 mM) for 24 h and analyzed for apoptosis markers using sub‑G1 population and Annexin V‑FITC/propidium iodide staining and DNA ladder formation. α‑glucosyl hesperidin treatment significantly reduced the severity of selenite‑induced cataract. The level of antioxidants was significantly reduced in the selenite‑treated rats compared with in the controls; however, they were normalized with α‑glucosyl hesperidin treatment. In vitro, hesperetin could significantly reduce the number of cells undergoing apoptosis induced by sodium selenite in human lens epithelial cell lines. Overall, oral consumption of α‑glucosyl hesperidin could delay the onset of selenite‑induced cataract, at least in part by modulating the selenite‑induced cell death in lens epithelial cells.

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