Open Access

Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model

  • Authors:
    • Yuling Lian
    • Fangui Zhao
    • Wenjun Wang
  • View Affiliations

  • Published online on: January 20, 2020     https://doi.org/10.3892/mmr.2020.10953
  • Pages: 1461-1470
  • Copyright: © Lian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study was designed to elucidate the underlying mechanisms of Bao Gui capsule (BGC) against hyperandrogenism, insulin resistance and leptin resistance of PCOS. Letrozole was used to induce a PCOS model in rats, which were then randomly divided into four groups (n=9): Control, Model, high‑dose BGC (BGC‑H) and low‑dose BGC (BGC‑L) group. Serum levels of follicle‑stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), estradiol (E2), insulin, leptin, and interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α (TNF‑α) in the hypothalamus were determined by ELISA. Protein levels of cytochrome P450c17α and cytochrome P450 aromatase (P450arom) in ovaries were determined by immunohistochemistry and western blot analysis. Additionally, the expression of GLUT4 in uterus and muscle tissue, and NF‑κB, IKKβ and SOCS3 mRNA levels in the hypothalamus were evaluated. BGC significantly reduced body weight gain and decreased serum levels of LH/FSH, T, log T/E2, insulin and leptin compared with the PCOS model rats. Furthermore, BGC markedly reduced the expression of P450c17α and significantly increased the expression of P450arom in ovaries, and increased the expression of GLUT4 in uterus and muscle tissues. BGC also effectively reduced the level of IL‑6 and TNF‑α, and the expression of IKKβ, NF‑κB and SOCS3 in the hypothalamus of PCOS model rats. These results suggest that BGC may effectively improve hyperandrogenism, insulin resistance, endometrial receptivity and the low‑grade chronic inflammation in the hypothalamus.
View Figures
View References

Related Articles

Journal Cover

March 2020
Volume 21 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Lian, Y., Zhao, F., & Wang, W. (2020). Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model. Molecular Medicine Reports, 21, 1461-1470. https://doi.org/10.3892/mmr.2020.10953
MLA
Lian, Y., Zhao, F., Wang, W."Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model". Molecular Medicine Reports 21.3 (2020): 1461-1470.
Chicago
Lian, Y., Zhao, F., Wang, W."Use of Bao Gui capsule in treatment of a polycystic ovary syndrome rat model". Molecular Medicine Reports 21, no. 3 (2020): 1461-1470. https://doi.org/10.3892/mmr.2020.10953