Open Access

Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway

  • Authors:
    • Xiangxiang Xu
    • Huajun Yu
    • Linxiao Sun
    • Chenlei Zheng
    • Yunfeng Shan
    • Zhenxu Zhou
    • Cheng Wang
    • Bicheng Chen
  • View Affiliations

  • Published online on: February 21, 2020     https://doi.org/10.3892/mmr.2020.10995
  • Pages: 1833-1840
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Adipose‑derived mesenchymal stem cells (ASCs) play a positive role in tissue injury repair and regeneration. The aim of this study was to determine whether ASCs could ameliorate chronic pancreatitis (CP) induced by the injection of dibutyltin dichloride (DBTC) and to elucidate its potential mechanisms. Furthermore, this study also explored whether there was a significant difference if the ASCs were injected via the inferior vena cava or the left gastric artery. CP was induced in rats by a single intravenous administration of DBTC, and the accumulation of collagen and apoptotic rates of pancreatic acinar cells were analyzed. According to the results, ASCs markedly reduced DBTC‑induced pancreatic damage and collagen deposition in the rat model of CP. Moreover, ASCs significantly decreased pancreatic cell apoptosis by regulating the expression levels of caspase‑3, BAX and Bcl‑2. These effects were observed regardless of whether the injection was in the inferior vena cava or the left gastric artery. It was also found that the expression levels of phosphorylated PI3K, AKT and mTOR in pancreatic tissues of the DBTC‑induced CP model group were significantly increased, while the expression levels of phosphorylated PI3K, AKT and mTOR in the two treatment groups were markedly decreased. ASCs noticeably suppressed the PI3K/AKT/mTOR pathway in the pancreatic tissue of DBTC‑induced CP. This study indicated that ASCs protect against pancreatic fibrosis by modulating the PI3K/AKT/mTOR pathway, and have the potential to be a new strategy for the treatment of CP in the future.
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April-2020
Volume 21 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xu X, Yu H, Sun L, Zheng C, Shan Y, Zhou Z, Wang C and Chen B: Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway. Mol Med Rep 21: 1833-1840, 2020
APA
Xu, X., Yu, H., Sun, L., Zheng, C., Shan, Y., Zhou, Z. ... Chen, B. (2020). Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway. Molecular Medicine Reports, 21, 1833-1840. https://doi.org/10.3892/mmr.2020.10995
MLA
Xu, X., Yu, H., Sun, L., Zheng, C., Shan, Y., Zhou, Z., Wang, C., Chen, B."Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 21.4 (2020): 1833-1840.
Chicago
Xu, X., Yu, H., Sun, L., Zheng, C., Shan, Y., Zhou, Z., Wang, C., Chen, B."Adipose‑derived mesenchymal stem cells ameliorate dibutyltin dichloride‑induced chronic pancreatitis by inhibiting the PI3K/AKT/mTOR signaling pathway". Molecular Medicine Reports 21, no. 4 (2020): 1833-1840. https://doi.org/10.3892/mmr.2020.10995