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Article Open Access

The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction

  • Authors:
    • Yun Shen
    • Yongchu Pan
    • Shuyu Guo
    • Lian Sun
    • Chi Zhang
    • Lin Wang
  • View Affiliations / Copyright

    Affiliations: Institute of Stomatology, Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2113-2122
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    Published online on: February 27, 2020
       https://doi.org/10.3892/mmr.2020.11006
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Abstract

The present study aimed to investigate whether the cytoskeleton, the Piezo1 ion channel and the transient receptor potential cation channel subfamily V member 4 (TRPV4) ion channel are equally functional in the mechanotransduction of periodontal ligament cells (PDLCs) and to reveal the interplay of these mechanically sensitive ion channels (MSCs). Human PDLCs (hPDLCs) were pretreated with cytochalasin D (the inhibitor of actin polymerization), GsMTx4 (the antagonist of Piezo1) and GSK205 (the antagonist of TRPV4), and then subjected to periodic mechanical loading. The expression levels of macrophage colony stimulating factor (M‑CSF), receptor activator of NF‑κB ligand (RANKL) and cyclooxygenase‑2 (COX2) in hPDLCs were detected via western blotting. Osteoblast mineralization induction capacity of the hPDLCs was also studied and the mitogen‑activated protein kinase (MAPK) expression profile was determined via protein microarray. The expression of Piezo1 and TRPV4 in the PDLCs was significantly increased at 8 h after loading. These differences in expression were accompanied by increased expression of M‑CSF, RANKL and COX2. Compared with the control group, key PDLC biomarkers were suppressed after mechanical loading following treatment with the inhibitors of Piezo1 (GsMTx4) and TRPV4 (GSK205). The phosphorylated‑MAPK protein array showed differential biomarker profiles among all groups. The present study suggested that both MSCs and the cytoskeleton participated as mechanical sensors, and did so independently in hPDLC mechanotransduction. Furthermore, the Piezo1 ion channel may transmit mechanical signals via the ERK signaling pathway; however, the TRPV4 channel may function via alternative signaling pathways.
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View References

1 

Proffit WR, Fields HW and Sarver DM: Contemporary Orthodontics. 5th. Mosby; St. Louis: 2012

2 

Chang M, Lin H, Fu H, Wang B, Han G and Fan M: MicroRNA-195-5p regulates osteogenic differentiation of periodontal ligament cells under mechanical loading. J Cell Physiol. 232:3762–3774. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Feng L, Zhang Y, Kou X, Yang R, Liu D, Wang X, Song Y, Cao H, He D, Gan Y and Zhou Y: Cadherin-11 modulates cell morphology and collagen synthesis in periodontal ligament cells under mechanical stress. Angle Orthod. 87:193–199. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Zhang H and Zhang D: Effects of periodontal ligament cells on alveolar bone metabolism under the action of force and inflammatory factors and its molecular mechanisms. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 39:432–437. 2017.PubMed/NCBI

5 

Li Y, Jacox LA, Little SH and Ko CC: Orthodontic tooth movement: The biology and clinical implications. Kaohsiung J Med Sci. 34:207–214. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Roca-Cusachs P, del Rio A, Puklin-Faucher E, Gauthier NC, Biais N and Sheetz MP: Integrin-dependent force transmission to the extracellular matrix by alpha-actinin triggers adhesion maturation. Proc Natl Acad Sci USA. 110:E1361–E1370. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Zhou J, Aponte-Santamaria C, Sturm S, Bullerjahn JT, Bronowska A and Grater F: Mechanism of focal adhesion kinase mechanosensing. PLoS Comput Biol. 11:e10045932015. View Article : Google Scholar : PubMed/NCBI

8 

Jin Y, Li J, Wang Y, Ye R, Feng X, Jing Z and Zhao Z: Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells. Angle Orthod. 85:87–94. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Lu J, Lee YK, Ran X, Lai WH, Li RA, Keung W, Tse K, Tse HF and Yao X: An abnormal TRPV4-related cytosolic Ca2+ rise in response to uniaxial stretch in induced pluripotent stem cells-derived cardiomyocytes from dilated cardiomyopathy patients. Biochim Biophys Acta Mol Basis Dis. 1863:2964–2972. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Cox CD, Bae C, Ziegler L, Hartley S, Nikolova-Krstevski V, Rohde PR, Ng CA, Sachs F, Gottlieb PA and Martinac B: Removal of the mechanoprotective influence of the cytoskeleton reveals PIEZO1 is gated by bilayer tension. Nat Commun. 7:103662016. View Article : Google Scholar : PubMed/NCBI

11 

Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, Dubin AE and Patapoutian A: Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science. 330:55–60. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Ge J, Li W, Zhao Q, Li N, Chen M, Zhi P, Li R, Gao N, Xiao B and Yang M: Architecture of the mammalian mechanosensitive Piezo1 channel. Nature. 527:64–69. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Strotmann R, Harteneck C, Nunnenmacher K, Schultz G and Plant TD: OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity. Nat Cell Biol. 2:695–702. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Güler AD, Lee H, Iida T, Shimizu I, Tominaga M and Caterina M: Heat-evoked activation of the ion channel, TRPV4. J Neurosci. 22:6408–6414. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Gao X, Wu L and O'Neil RG: Temperature-modulated diversity of TRPV4 channel gating: Activation by physical stresses and phorbol ester derivatives through protein kinase C-dependent and -independent pathways. J Biol Chem. 278:27129–27137. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Danielczok JG, Terriac E, Hertz L, Petkova-Kirova P, Lautenschlager F, Laschke MW and Kaestner L: Red blood cell passage of small capillaries is associated with transient Ca2+-mediated adaptations. Front Physiol. 8:9792017. View Article : Google Scholar : PubMed/NCBI

17 

Servin-Vences MR, Richardson J, Lewin GR and Poole K: Mechanoelectrical transduction in chondrocytes. Clin Exp Pharmacol Physiol. 45:481–488. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Sugimoto A, Miyazaki A, Kawarabayashi K, Shono M, Akazawa Y, Hasegawa T, Ueda-Yamaguchi K, Kitamura T, Yoshizaki K, Fukumoto S and Iwamoto T: Piezo type mechanosensitive ion channel component 1 functions as a regulator of the cell fate determination of mesenchymal stem cells. Sci Rep. 7:176962017. View Article : Google Scholar : PubMed/NCBI

19 

Blythe NM, Muraki K, Ludlow MJ, Stylianidis V, Gilbert HTJ, Evans EL, Cuthbertson K, Foster R, Swift J, Li J, et al: Mechanically activated Piezo1 channels of cardiac fibroblasts stimulate p38 mitogen-activated protein kinase activity and interleukin-6 secretion. J Biol Chem. 294:17395–17408. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Li XF, Zhang Z, Li XD, Wang TB and Zhang HN: Mechanism of the Piezo1 protein-induced apoptosis of the chondrocytes through the MAPK/ERK1/2 signal pathway. Zhonghua Yi Xue Za Zhi. 96:2472–2477. 2016.(In Chinese). PubMed/NCBI

21 

Liang J, Huang B, Yuan G, Chen Y, Liang F, Zeng H, Zheng S, Cao L, Geng D and Zhou S: Stretch-activated channel Piezo1 is up-regulated in failure heart and cardiomyocyte stimulated by AngII. Am J Transl Res. 9:2945–2955. 2017.PubMed/NCBI

22 

Qu YJ, Zhang X, Fan ZZ, Huai J, Teng YB, Zhang Y and Yue SW: Effect of TRPV4-p38 MAPK pathway on neuropathic pain in rats with chronic compression of the dorsal root ganglion. Biomed Res Int. 2016:69789232016. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Yang Z, Tan S, Shen Y, Chen R, Wu C, Xu Y, Song Z and Fu Q: Inhibition of FSS-induced actin cytoskeleton reorganization by silencing LIMK2 gene increases the mechanosensitivity of primary osteoblasts. Bone. 74:182–190. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Kanzaki H, Chiba M, Shimizu Y and Mitani H: Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J Bone Miner Res. 17:210–220. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Boyce BF and Xing L: The RANKL/RANK/OPG pathway. Curr Osteoporos Rep. 5:98–104. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Garlet TP, Coelho U, Silva JS and Garlet GP: Cytokine expression pattern in compression and tension sides of the periodontal ligament during orthodontic tooth movement in humans. Eur J Oral Sci. 115:355–362. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Kim T, Handa A, Iida J and Yoshida S: RANKL expression in rat periodontal ligament subjected to a continuous orthodontic force. Arch Oral Biol. 52:244–250. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Kanjanamekanant K, Luckprom P and Pavasant P: Mechanical stress-induced interleukin-1beta expression through adenosine triphosphate/P2X7 receptor activation in human periodontal ligament cells. J Periodontal Res. 48:169–176. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Fu HD, Wang BK, Wan ZQ, Lin H, Chang ML and Han GL: Wnt5a mediated canonical Wnt signaling pathway activation in orthodontic tooth movement: Possible role in the tension force-induced bone formation. J Mol Histol. 47:455–466. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Alhashimi N, Frithiof L, Brudvik P and Bakhiet M: CD40-CD40L expression during orthodontic tooth movement in rats. Angle Orthod. 74:100–105. 2004.PubMed/NCBI

32 

Grieve WG 3rd, Johnson GK, Moore RN, Reinhardt RA and DuBois LM: Prostaglandin E (PGE) and interleukin-1 beta (IL-1 beta) levels in gingival crevicular fluid during human orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 105:369–374. 1994. View Article : Google Scholar : PubMed/NCBI

33 

Dougall WC: Molecular pathways: Osteoclast-dependent and osteoclast-independent roles of the RANKL/RANK/OPG pathway in tumorigenesis and metastasis. Clin Cancer Res. 18:326–335. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Sachs F: Mechanical transduction by ion channels: A cautionary tale. World J Neurol. 5:74–87. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Ingber DE: Tensegrity-based mechanosensing from macro to micro. Prog Biophys Mol Biol. 97:163–179. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Li J, Chen G, Zheng L, Luo S and Zhao Z: Osteoblast cytoskeletal modulation in response to compressive stress at physiological levels. Mol Cell Biochem. 304:45–52. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Mammoto A and Ingber DE: Cytoskeletal control of growth and cell fate switching. Curr Opin Cell Biol. 21:864–870. 2009. View Article : Google Scholar : PubMed/NCBI

38 

Son GY, Yang YM, Park WS, Chang I and Shin DM: Hypotonic stress induces RANKL via transient receptor potential melastatin 3 (TRPM3) and vaniloid 4 (TRPV4) in human PDL cells. J Dent Res. 94:473–481. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Choquet D, Felsenfeld DP and Sheetz MP: Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages. Cell. 88:39–48. 1997. View Article : Google Scholar : PubMed/NCBI

40 

Matthews BD, Thodeti CK, Tytell JD, Mammoto A, Overby DR and Ingber DE: Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface beta1 integrins. Integr Biol (Camb). 2:435–442. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Berridge MJ, Bootman MD and Lipp P: Calcium-a life and death signal. Nature. 395:645–648. 1998. View Article : Google Scholar : PubMed/NCBI

42 

Berridge MJ, Lipp P and Bootman MD: The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 1:11–21. 2000. View Article : Google Scholar : PubMed/NCBI

43 

Benavides Damm T and Egli M: Calcium's role in mechanotransduction during muscle development. Cell Physiol Biochem. 33:249–272. 2014. View Article : Google Scholar : PubMed/NCBI

44 

Strotmann R, Schultz G and Plant TD: Ca2+-dependent potentiation of the nonselective cation channel TRPV4 is mediated by a C-terminal calmodulin binding site. J Biol Chem. 278:26541–26549. 2003. View Article : Google Scholar : PubMed/NCBI

45 

Ishida T, Peterson TE, Kovach NL and Berk BC: MAP kinase activation by flow in endothelial cells. Role of beta 1 integrins and tyrosine kinases. Circ Res. 79:310–316. 1996. View Article : Google Scholar : PubMed/NCBI

46 

Kippenberger S, Bernd A, Loitsch S, Guschel M, Muller J, Bereiter-Hahn J and Kaufmann R: Signaling of mechanical stretch in human keratinocytes via MAP kinases. J Invest Dermatol. 114:408–412. 2000. View Article : Google Scholar : PubMed/NCBI

47 

Yamazaki T, Komuro I, Shiojima I and Yazaki Y: The molecular mechanism of cardiac hypertrophy and failure. Ann N Y Acad Sci. 874:38–48. 1999. View Article : Google Scholar : PubMed/NCBI

48 

Wang Q, Zhou Y, Wang X and Evers BM: Glycogen synthase kinase-3 is a negative regulator of extracellular signal-regulated kinase. Oncogene. 25:43–50. 2006. View Article : Google Scholar : PubMed/NCBI

49 

Liu S, Fang X, Hall H, Yu S, Smith D, Lu Z, Fang D, Liu J, Stephens LC, Woodgett JR and Mills GB: Homozygous deletion of glycogen synthase kinase 3beta bypasses senescence allowing Ras transformation of primary murine fibroblasts. Proc Natl Acad Sci USA. 105:5248–5253. 2008. View Article : Google Scholar : PubMed/NCBI

50 

Götschel F, Kern C, Lang S, Sparna T, Markmann C, Schwager J, McNelly S, von Weizsäcker F, Laufer S, Hecht A and Merfort I: Inhibition of GSK3 differentially modulates NF-kappaB, CREB, AP-1 and beta-catenin signaling in hepatocytes, but fails to promote TNF-alpha-induced apoptosis. Exp Cell Res. 314:1351–1366. 2008. View Article : Google Scholar : PubMed/NCBI

51 

Terada Y, Nakashima O, Inoshita S, Kuwahara M, Sasaki S and Marumo F: Mitogen-activated protein kinase cascade and transcription factors: The opposite role of MKK3/6-p38K and MKK1-MAPK. Nephrol Dial Transplant. 14 (Suppl 1):S45–S47. 1999. View Article : Google Scholar

52 

Papadopoulou A, Todaro A, Eliades T and Kletsas D: Effect of hyperglycaemic conditions on the response of human periodontal ligament fibroblasts to mechanical stretching. Eur J Orthod. 41:583–590. 2019. View Article : Google Scholar : PubMed/NCBI

53 

Danciu TE, Adam RM, Naruse K, Freeman MR and Hauschka PV: Calcium regulates the PI3K-Akt pathway in stretched osteoblasts. FEBS Lett. 536:193–197. 2003. View Article : Google Scholar : PubMed/NCBI

54 

Divolis G, Mavroeidi P, Mavrofrydi O and Papazafiri P: Differential effects of calcium on PI3K-Akt and HIF-1α survival pathways. Cell Biol Toxicol. 32:437–449. 2016. View Article : Google Scholar : PubMed/NCBI

55 

Hdud IM, Mobasheri A and Loughna PT: Effect of osmotic stress on the expression of TRPV4 and BKCa channels and possible interaction with ERK1/2 and p38 in cultured equine chondrocytes. Am J Physiol Cell Physiol. 306:C1050–C1057. 2014. View Article : Google Scholar : PubMed/NCBI

56 

Qi YX, Qu MJ, Yan ZQ, Zhao D, Jiang XH, Shen BR and Jiang ZL: Cyclic strain modulates migration and proliferation of vascular smooth muscle cells via Rho-GDIalpha, Rac1, and p38 pathway. J Cell Biochem. 109:906–914. 2010.PubMed/NCBI

57 

Zheng L, Huang Y, Song W, Gong X, Liu M, Jia X, Zhou G, Chen L, Li A and Fan Y: Fluid shear stress regulates metalloproteinase-1 and 2 in human periodontal ligament cells: Involvement of extracellular signal-regulated kinase (ERK) and P38 signaling pathways. J Biomech. 45:2368–2375. 2012. View Article : Google Scholar : PubMed/NCBI

58 

Chen Y, Yang K, Zhou Z, Wang L, Du Y and Wang X: Mechanical stress modulates the RANKL/OPG system of periodontal ligament stem cells via α7 nAChR in human deciduous teeth: An in vitro study. Stem Cells Int. 2019:53263412019. View Article : Google Scholar : PubMed/NCBI

59 

Manokawinchoke J, Pavasant P and Osathanon T: Intermittent compressive stress regulates Notch target gene expression via transforming growth factor-β signaling in murine pre-osteoblast cell line. Arch Oral Biol. 82:47–54. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shen Y, Pan Y, Guo S, Sun L, Zhang C and Wang L: The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Mol Med Rep 21: 2113-2122, 2020.
APA
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., & Wang, L. (2020). The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Molecular Medicine Reports, 21, 2113-2122. https://doi.org/10.3892/mmr.2020.11006
MLA
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., Wang, L."The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction". Molecular Medicine Reports 21.5 (2020): 2113-2122.
Chicago
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., Wang, L."The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction". Molecular Medicine Reports 21, no. 5 (2020): 2113-2122. https://doi.org/10.3892/mmr.2020.11006
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Y, Pan Y, Guo S, Sun L, Zhang C and Wang L: The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Mol Med Rep 21: 2113-2122, 2020.
APA
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., & Wang, L. (2020). The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction. Molecular Medicine Reports, 21, 2113-2122. https://doi.org/10.3892/mmr.2020.11006
MLA
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., Wang, L."The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction". Molecular Medicine Reports 21.5 (2020): 2113-2122.
Chicago
Shen, Y., Pan, Y., Guo, S., Sun, L., Zhang, C., Wang, L."The roles of mechanosensitive ion channels and associated downstream MAPK signaling pathways in PDLC mechanotransduction". Molecular Medicine Reports 21, no. 5 (2020): 2113-2122. https://doi.org/10.3892/mmr.2020.11006
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