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Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway

  • Authors:
    • Zengxin Jiang
    • Qingmin Zeng
    • Defang Li
    • Lei Ding
    • Wei Lu
    • Mengxuan Bian
    • Jingping Wu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Fudan University Jinshan Hospital, Shanghai 201508, P.R. China, Department of Orthopedic Surgery, Shanghai TCM‑Integrated Hospital, Shanghai University of TCM, Shanghai 200082, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2220-2226
    |
    Published online on: March 3, 2020
       https://doi.org/10.3892/mmr.2020.11009
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Abstract

Diabetes mellitus (DM) contributes to intervertebral disc degeneration (IDD). The long non‑coding RNA MALAT1 has been revealed to play an important role in diabetes‑associated complications. However, the specific role of MALAT1 in diabetes‑associated IDD has not been determined. The aim of the present study was to evaluate the roles of MALAT1 in the apoptosis of cartilage endplate (CEP) cells induced by high glucose and to explore the mechanisms underlying this effect. Rat CEP cells were cultured in high‑glucose medium (25 mM glucose) for 24 or 72 h. Cells cultured in medium containing 5 mM glucose were used as a control. Flow cytometry was used to detect the degree of apoptosis. Reverse transcription‑quantitative PCR was used to measure the expression of MALAT1 mRNA. In addition, CEP cells were treated with different conditions (high glucose, high glucose + MALAT1 negative control, high glucose + MALAT1 RNAi, normal control) for 72 h. Flow cytometry was subsequently used to detect apoptosis and western blotting was used to measure the expression levels of total and phosphorylated p38. The results revealed that high glucose concentration promoted apoptosis and enhanced expression of MALAT1 in CEP cells. Furthermore, MALAT1 knockout decreased the expression levels of total and phosphorylated p38 and reduced the apoptosis of rat CEP cells. The results obtained in the present study indicated that MALAT1 may serve as an important therapeutic target for curing or delaying IDD in patients with diabetes.
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1 

Dowdell J, Erwin M, Choma T, Vaccaro A, Iatridis J and Cho SK: Intervertebral Disk Degeneration and Repair. Neurosurgery. 80 (Suppl 3):S46–S54. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Gu W, Zhu Q, Gao X and Brown MD: Simulation of the Progression of Intervertebral Disc Degeneration due to Decreased Nutrition Supply. Spine. 39:E1411–E1417. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Zhu Q, Gao X, Levene HB, Brown MD and Gu W: Influences of Nutrition Supply and Pathways on the Degenerative Patterns in Human Intervertebral Disc. Spine. 41:568–576. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Määttä JH, Kraatari M, Wolber L, Niinimäki J, Wadge S, Karppinen J and Williams FM: Vertebral endplate change as a feature of intervertebral disc degeneration: A heritability study. Eur Spine J. 23:1856–1862. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Wang Y, Videman T and Battié MC: ISSLS prize winner: Lumbar vertebral endplate lesions: associations with disc degeneration and back pain history. Spine. 37:1490–1496. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Kang R, Li H, Ringgaard S, Rickers K, Sun H, Chen M, Xie L and Bünger C: Interference in the endplate nutritional pathway causes intervertebral disc degeneration in an immature porcine model. Int Orthop. 38:1011–1017. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Yin S, Du H, Zhao W, Ma S, Zhang M, Guan M and Liu M: Inhibition of both endplate nutritional pathways results in intervertebral disc degeneration in a goat model. J Orthop Surg Res. 14:1382019. View Article : Google Scholar : PubMed/NCBI

8 

Hutton WC, Murakami H, Li J, Elmer WA, Yoon ST, Minamide A, Akamaru T and Tomita K: The effect of blocking a nutritional pathway to the intervertebral disc in the dog model. J Spinal Disord Tech. 17:53–63. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Fields AJ, Berg-Johansen B, Metz LN, Miller S, La B, Liebenberg EC, Coughlin DG, Graham JL, Stanhope KL, Havel PJ, et al: Alterations in intervertebral disc composition, matrix homeostasis and biomechanical behavior in the UCD-T2DM rat model of type 2 diabetes. J Orthop Res. 33:738–746. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Agius R, Galea R and Fava S: Bone mineral density and intervertebral disc height in type 2 diabetes. J Diabetes Complications. 30:644–650. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Sakellaridis N: The influence of diabetes mellitus on lumbar intervertebral disk herniation. Surg Neurol. 66:152–154. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Jiang Z, Lu W, Zeng Q, Li D, Ding L and Wu J: High glucose-induced excessive reactive oxygen species promote apoptosis through mitochondrial damage in rat cartilage endplate cells. J Orthop Res. 36:2476–2483. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Jathar S, Kumar V, Srivastava J and Tripathi V: Technological Developments in lncRNA Biology. Adv Exp Med Biol. 1008:283–323. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Kung JT, Colognori D and Lee JT: Long noncoding RNAs: Past, present, and future. Genetics. 193:651–669. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Jarroux J, Morillon A and Pinskaya M: History, Discovery, and Classification of lncRNAs. Adv Exp Med Biol. 1008:1–46. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Chen WK, Yu XH, Yang W, Wang C, He WS, Yan YG, Zhang J and Wang WJ: lncRNAs: Novel players in intervertebral disc degeneration and osteoarthritis. Cell Prolif. 50:e123132017. View Article : Google Scholar

17 

Zhang X, Hamblin MH and Yin KJ: The long noncoding RNA Malat1: Its physiological and pathophysiological functions. RNA Biol. 14:1705–1714. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Li ZX, Zhu QN, Zhang HB, Hu Y, Wang G and Zhu YS: MALAT1: A potential biomarker in cancer. Cancer Manag Res. 10:6757–6768. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Abdulle LE, Hao JL, Pant OP, Liu XF, Zhou DD, Gao Y, Suwal A and Lu CW: MALAT1 as a Diagnostic and Therapeutic Target in Diabetes-Related Complications: A Promising Long-Noncoding RNA. Int J Med Sci. 16:548–555. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Gong Y, Zhu Y, Zhu B, Si X, Heng D, Tang Y, Sun X and Lin L: LncRNA MALAT1 is up-regulated in diabetic gastroparesis and involved in high-glucose-induced cellular processes in human gastric smooth muscle cells. Biochem Biophys Res Commun. 496:401–406. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Yan B, Tao ZF, Li XM, Zhang H, Yao J and Jiang Q: Aberrant expression of long noncoding RNAs in early diabetic retinopathy. Invest Ophthalmol Vis Sci. 55:941–951. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Zhang M, Gu H, Xu W and Zhou X: Down-regulation of lncRNA MALAT1 reduces cardiomyocyte apoptosis and improves left ventricular function in diabetic rats. Int J Cardiol. 203:214–216. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Hu M, Wang R, Li X, Fan M, Lin J, Zhen J, Chen L and Lv Z: LncRNA MALAT1 is dysregulated in diabetic nephropathy and involved in high glucose-induced podocyte injury via its interplay with β-catenin. J Cell Mol Med. 21:2732–2747. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Gong W, Zhu G, Li J and Yang X: LncRNA MALAT1 promotes the apoptosis and oxidative stress of human lens epithelial cells via p38MAPK pathway in diabetic cataract. Diabetes Res Clin Pract. 144:314–321. 2018. View Article : Google Scholar : PubMed/NCBI

25 

Kim EK and Choi EJ: Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 1802:396–405. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Sui X, Kong N, Ye L, Han W, Zhou J, Zhang Q, He C and Pan H: p38 and JNK MAPK pathways control the balance of apoptosis and autophagy in response to chemotherapeutic agents. Cancer Lett. 344:174–179. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Cuadrado A and Nebreda AR: Mechanisms and functions of p38 MAPK signalling. Biochem J. 429:403–417. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Liu JY, Yao J, Li XM, Song YC, Wang XQ, Li YJ, Yan B and Jiang Q: Pathogenic role of lncRNA-MALAT1 in endothelial cell dysfunction in diabetes mellitus. Cell Death Dis. 5:e15062014. View Article : Google Scholar : PubMed/NCBI

29 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

30 

Teraguchi M, Yoshimura N, Hashizume H, Yamada H, Oka H, Minamide A, Nagata K, Ishimoto Y, Kagotani R, Kawaguchi H, et al: Progression, incidence, and risk factors for intervertebral disc degeneration in a longitudinal population-based cohort: The Wakayama Spine Study. Osteoarthritis Cartilage. 25:1122–1131. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Jiang L, Zhang X, Zheng X, Ru A, Ni X, Wu Y, Tian N, Huang Y, Xue E, Wang X, et al: Apoptosis, senescence, and autophagy in rat nucleus pulposus cells: Implications for diabetic intervertebral disc degeneration. J Orthop Res. 31:692–702. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Shan L, Yang D, Zhu D, Feng F and Li X: High glucose promotes annulus fibrosus cell apoptosis through activating the JNK and p38 MAPK pathways. Biosci Rep. 39:BSR201908532019. View Article : Google Scholar : PubMed/NCBI

33 

Wachtel TJ: The diabetic hyperosmolar state. Clin Geriatr Med. 6:797–806. 1990. View Article : Google Scholar : PubMed/NCBI

34 

Kitabchi AE and Nyenwe EA: Hyperglycemic crises in diabetes mellitus: Diabetic ketoacidosis and hyperglycemic hyperosmolar state. Endocrinol Metab Clin North Am. 35725–751. (viii)2006. View Article : Google Scholar : PubMed/NCBI

35 

Umpierrez G and Korytkowski M: Diabetic emergencies - ketoacidosis, hyperglycaemic hyperosmolar state and hypoglycaemia. Nat Rev Endocrinol. 12:222–232. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang Z, Zeng Q, Li D, Ding L, Lu W, Bian M and Wu J: Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway. Mol Med Rep 21: 2220-2226, 2020.
APA
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., & Wu, J. (2020). Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway. Molecular Medicine Reports, 21, 2220-2226. https://doi.org/10.3892/mmr.2020.11009
MLA
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., Wu, J."Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway". Molecular Medicine Reports 21.5 (2020): 2220-2226.
Chicago
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., Wu, J."Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway". Molecular Medicine Reports 21, no. 5 (2020): 2220-2226. https://doi.org/10.3892/mmr.2020.11009
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Z, Zeng Q, Li D, Ding L, Lu W, Bian M and Wu J: Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway. Mol Med Rep 21: 2220-2226, 2020.
APA
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., & Wu, J. (2020). Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway. Molecular Medicine Reports, 21, 2220-2226. https://doi.org/10.3892/mmr.2020.11009
MLA
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., Wu, J."Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway". Molecular Medicine Reports 21.5 (2020): 2220-2226.
Chicago
Jiang, Z., Zeng, Q., Li, D., Ding, L., Lu, W., Bian, M., Wu, J."Long non‑coding RNA MALAT1 promotes high glucose‑induced rat cartilage endplate cell apoptosis via the p38/MAPK signalling pathway". Molecular Medicine Reports 21, no. 5 (2020): 2220-2226. https://doi.org/10.3892/mmr.2020.11009
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