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Article

MicroRNA‑196b targets COSMC in pediatric IgA nephropathy

  • Authors:
    • Qiang Sun
    • Jingchao Lan
    • Hong Zhang
    • Nan Zhou
    • Ying Liang
    • Xiaorong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing Key Laboratory of Pediatric Chronic Kidney Disease and Blood Purification, Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing 100045, P.R. China, Pediatric Department, Shunyi Women's and Children's Hospital of Beijing Children's Hospital, Beijing 101300, P.R. China
  • Pages: 2260-2266
    |
    Published online on: March 9, 2020
       https://doi.org/10.3892/mmr.2020.11015
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Abstract

The aim of the present study was to investigate the effect of microRNAs (miRNAs) on the expression level of core1β3‑galactosyltransferase‑specific molecular chaperone (COSMC) in immunoglobulin A nephropathy (IgAN). miRNA expression levels were determined in pediatric patients with IgAN (IgAN group), in patients with other renal diseases (control group) and healthy pediatrics (control group). The target miRNAs of COSMC were investigated in the present study. Western blot analysis was performed to examine the effects of miRNAs on COSMC expression levels. In addition, galactose‑deficient IgA1 (Gd‑IgA1) expression levels were detected following the addition of miRNA‑196b. The present results suggested that the expression levels of 205 miRNAs significantly differed between the IgAN and healthy control groups. The present results also suggested that miRNA‑196b and miRNA‑33a‑3p targeted COSMC, and that miRNA‑196b expression in B lymphocytes was significantly higher in the IgAN group compared with the control group (P<0.0001). However, COSMC expression level was significantly downregulated in isolated B lymphocytes transfected with miRNA‑196b mimics, but Gd‑IgA1 expression levels were increased. Therefore, miRNA‑196b may play a role in the formation of Gd‑IgA1 and IgAN pathogenesis via COSMC regulation.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Q, Lan J, Zhang H, Zhou N, Liang Y and Liu X: MicroRNA‑196b targets COSMC in pediatric IgA nephropathy. Mol Med Rep 21: 2260-2266, 2020.
APA
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., & Liu, X. (2020). MicroRNA‑196b targets COSMC in pediatric IgA nephropathy. Molecular Medicine Reports, 21, 2260-2266. https://doi.org/10.3892/mmr.2020.11015
MLA
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., Liu, X."MicroRNA‑196b targets COSMC in pediatric IgA nephropathy". Molecular Medicine Reports 21.5 (2020): 2260-2266.
Chicago
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., Liu, X."MicroRNA‑196b targets COSMC in pediatric IgA nephropathy". Molecular Medicine Reports 21, no. 5 (2020): 2260-2266. https://doi.org/10.3892/mmr.2020.11015
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Q, Lan J, Zhang H, Zhou N, Liang Y and Liu X: MicroRNA‑196b targets COSMC in pediatric IgA nephropathy. Mol Med Rep 21: 2260-2266, 2020.
APA
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., & Liu, X. (2020). MicroRNA‑196b targets COSMC in pediatric IgA nephropathy. Molecular Medicine Reports, 21, 2260-2266. https://doi.org/10.3892/mmr.2020.11015
MLA
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., Liu, X."MicroRNA‑196b targets COSMC in pediatric IgA nephropathy". Molecular Medicine Reports 21.5 (2020): 2260-2266.
Chicago
Sun, Q., Lan, J., Zhang, H., Zhou, N., Liang, Y., Liu, X."MicroRNA‑196b targets COSMC in pediatric IgA nephropathy". Molecular Medicine Reports 21, no. 5 (2020): 2260-2266. https://doi.org/10.3892/mmr.2020.11015
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