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Article Open Access

Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure

  • Authors:
    • Mitsuru Chiba
    • Haruka Uehara
    • Ikumi Niiyama
    • Haruka Kuwata
    • Satoru Monzen
  • View Affiliations / Copyright

    Affiliations: Department of Bioscience and Laboratory Medicine, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan, Department of Medical Technology, School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan, Department of Radiation Science, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan
    Copyright: © Chiba et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2452-2458
    |
    Published online on: April 2, 2020
       https://doi.org/10.3892/mmr.2020.11054
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Abstract

The small intestine is one of the most highly regenerative and radiosensitive tissues in mammals, including humans. Exposure to high doses of ionizing radiation causes serious intestinal damage. Recently, several investigations have been conducted using radioprotective agents to determine ways for reducing intestinal damage caused by radiation exposure. However, a thorough understanding of functional changes occurring in the small intestine of mice exposed to high‑dose radiation is necessary for developing novel and more potent radioprotective agents. In this study, we examined changes in microRNA (miRNA/miR) expressions in the small intestine of mice at 72 h after X‑ray exposure (10 Gy). We identified seven upregulated miRNAs and six downregulated miRNAs in the small intestine of mice following radiation exposure using miRNA microarray analysis. Particularly, miR‑34a‑5p was highly expressed, which was confirmed by reverse transcription-quantitative PCR. Forkhead box P1 (Foxp1) was predicted to be a target of the mRNA of miR‑34a‑5p using OmicsNet. Decreased Foxp1 expression in the small intestine following radiation exposure was confirmed, suggesting that Foxp1 expression recovery may induce the suppression of radiation‑induced enteritis. Therefore, miR‑34a‑5p is a potential target molecule for developing novel radioprotective agents.
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1 

International Atomic Energy Agency, . Diagnosis and treatment of radiation injures. IAEA Safety Report Series. 2:IAEA. (Vienna). 1998.

2 

Metcalfe C, Kljavin NM, Ybarra R and de Sauvage FJ: Lgr5+ stem cells are indispensable for radiation-induced intestinal regeneration. Cell Stem Cell. 14:149–159. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Leibowitz BJ, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T and Yu J: Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice. Nat Commun. 5:34942014. View Article : Google Scholar : PubMed/NCBI

4 

Berger ME, Christensen DM, Lowry PC, Jones OW and Wiley AL: Medical management of radiation injuries: Current approaches. Occup Med (Lond). 56:162–172. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Macià I Garau M, Lucas Calduch A and López EC: Radiobiology of the acute radiation syndrome. Rep Pract Oncol Radiother. 16:123–130. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Tanaka SI: Summary of the JCO criticality accident in Tokai-mura and a dose assessment. J Radiat Res. 42 (Suppl):S1–S9. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Sasaki MS, Hayata I, Kamada N, Kodama Y and Kodama S: Chromosome aberration analysis in persons exposed to low-level radiation from the JCO criticality accident in Tokai-mura. J Radiat Res. 42:S107–S116. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Asano S: Current status of hematopoietic stem cell transplantation for acute radiation syndromes. Int J Hematol. 95:227–231. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Verginadis II, Kanade R, Bell B, Koduri S, Ben-Josef E and Koumenis C: A novel mouse model to study image-guided, radiation-induced intestinal injury and preclinical screening of radioprotectors. Cancer Res. 77:908–917. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Yamamoto T, Kinoshita M, Shinomiya N, Hiroi S, Sugasawa H, Matsushita Y, Majima T, Saitoh D and Seki S: Pretreatment with ascorbic acid prevents lethal gastrointestinal syndrome in mice receiving a massive amount of radiation. J Radiat Res. 51:145–156. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Mohr AM and Mott JL: Overview of microRNA biology. Semin Liver Dis. 35:3–11. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Chiba M, Miura T, Kasai K, Monzen S, Kashiwakura I, Yasue H and Nakamura T: Identification of up-regulated and down-regulated cis-natural antisense transcripts in the human B lymphoblastic cell line IM-9 after X-ray irradiation. Mol Med Rep. 5:1151–1157. 2012.PubMed/NCBI

13 

Chiba M: Radiation-responsive transcriptome analysis in human lymphoid cells. Radiat Prot Dosimetry. 152:164–167. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Strup-Perrot C, Mathé D, Linard C, Violot D, Milliat F, François A, Bourhis J and Vozenin-Brotons MC: Global gene expression profiles reveal an increase in mRNA levels of collagens, MMPs, and TIMPs in late radiation enteritis. Am J Physiol Gastrointest Liver Physiol. 287:G875–G885. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Zheng J, Wang J, Pouliot M, Authier S, Zhou D, Loose DS and Hauer-Jensen M: Gene expression profiling in non-human primate jejunum, ileum and colon after total-body irradiation: A comparative study of segment-specific molecular and cellular responses. BMC Genomics. 16:9842015. View Article : Google Scholar : PubMed/NCBI

16 

Chiba M, Kubota S, Sakai A and Monzen S: Cell-to-cell communication via extracellular vesicles among human pancreatic cancer cells derived from the same patient. Mol Med Rep. 18:3989–3996. 2018.PubMed/NCBI

17 

Liu Z, Liu H, Jiang J, Tan S, Yang Y, Zhan Y and Wu B: PDGF-BB and bFGF ameliorate radiation-induced intestinal progenitor/stem cell apoptosis via Akt/p53 signaling in mice. Am J Physiol Gastrointest Liver Physiol. 307:G1033–G1043. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ and Clevers H: Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 449:1003–1007. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Hermeking H: The miR-34 family in cancer and apoptosis. Cell Death Differ. 17:193–199. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Misso G, Di Martino MT, De Rosa G, Farooqi AA, Lombardi A, Campani V, Zarone MR, Gullà A, Tagliaferri P, Tassone P and Caraglia M: Mir-34: A new weapon against cancer? Mol Ther Nucleic Acids. 3:e1942014. View Article : Google Scholar : PubMed/NCBI

21 

He L, He X, Lim LP, de Stanchina E, Xuan Z, Liang Y, Xue W, Zender L, Magnus J, Ridzon D, et al: A microRNA component of the p53 tumour suppressor network. Nature. 447:1130–1134. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Tarasov V, Jung P, Verdoodt B, Lodygin D, Epanchintsev A, Menssen A, Meister G and Hermeking H: Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrest. Cell Cycle. 6:1586–1593. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Stankevicins L, Almeida da Silva AP, Ventura Dos Passos F, Dos Santos Ferreira E, Menks Ribeiro MC, G David M, J Pires E, Ferreira-Machado SC, Vassetzky Y, de Almeida CE and de Moura Gallo CV: MiR-34a is up-regulated in response to low dose, low energy X-ray induced DNA damage in breast cells. Radiat Oncol. 8:2312013. View Article : Google Scholar : PubMed/NCBI

24 

Salzman DW, Nakamura K, Nallur S, Dookwah MT, Metheetrairut C, Slack FJ and Weidhaas JB: miR-34 activity is modulated through 5′-end phosphorylation in response to DNA damage. Nat Commun. 7:109542016. View Article : Google Scholar : PubMed/NCBI

25 

John-Aryankalayil M, Palayoor ST, Makinde AY, Cerna D, Simone CB II, Falduto MT, Magnuson SR and Coleman CN: Fractionated radiation alters oncomir and tumor suppressor miRNAs in human prostate cancer cells. Radiat Res. 178:105–117. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Girardi C, De Pittà C, Casara S, Sales G, Lanfranchi G, Celotti L and Mognato M: Analysis of miRNA and mRNA expression profiles highlights alterations in ionizing radiation response of human lymphocytes under modeled microgravity. PLoS One. 7:e312932012. View Article : Google Scholar : PubMed/NCBI

27 

Liu C, Zhou C, Gao F, Cai S, Zhang C, Zhao L, Zhao F, Cao F, Lin J, Yang Y, et al: MiR-34a in age and tissue related radio-sensitivity and serum miR-34a as a novel indicator of radiation injury. Int J Biol Sci. 7:221–233. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Halimi M, Shahabi A, Moslemi D, Parsian H, Shari SM, Satire R, Yeganeh F and Zabihi E: Human serum miR-34a as an indicator of exposure to ionizing radiation. Radiat Environ Biophys. 55:423–429. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Usui N, Araujo DJ, Kulkarni A, Co M, Ellegood J, Harper M, Toriumi K, Lerch JP and Konopka G: Foxp1 regulation of neonatal vocalizations via cortical development. Genes Dev. 31:2039–2055. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Gascoyne DM and Banham AH: The significance of FOXP1 in diffuse large B-cell lymphoma. Leuk Lymphoma. 58:1037–1051. 2017. View Article : Google Scholar : PubMed/NCBI

31 

De Smedt L, Palmans S, Govaere O, Moisse M, Boeckx B, De Hertogh G, Prenen H, Van Cutsem E, Tejpar S, Tousseyn T and Sagaert X: Expression of FOXP1 and colorectal cancer prognosis. Lab Med. 46:299–311. 2015. View Article : Google Scholar : PubMed/NCBI

32 

He LX, Wang JB, Sun B, Zhao J, Li L, Xu T, Li H, Sun JQ, Ren J, Liu R, et al: Suppression of TNF-α and free radicals reduces systematic inflammatory and metabolic disorders: Radioprotective effects of ginseng oligopeptides on intestinal barrier function and antioxidant defense. J Nutr Biochem. 40:53–61. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Kalita B, Ranjan R, Singh A, Yashavarddhan MH, Bajaj S and Gupta ML: A combination of podophyllotoxin and rutin attenuates radiation induced gastrointestinal injury by negatively regulating NF-κB/p53 signaling in lethally irradiated mice. PLoS One. 11:e01685252016. View Article : Google Scholar : PubMed/NCBI

34 

Tas S, Ozkan OF, Cikman O, Kiraz A, Akgun Y and Karaayvaz M: L-carnitine has a protective effect on the colonic mucosa during abdominopelvic radiotherapy in rats. Acta Cir Bras. 31:615–620. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Jeong BK, Song JH, Jeong H, Choi HS, Jung JH, Hahm JR, Woo SH, Jung MH, Choi BH, Kim JH and Kang KM: Effect of alpha-lipoic acid on radiation-induced small intestine injury in mice. Oncotarget. 7:15105–15117. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Chiba M, Monzen S, Iwaya C, Kashiwagi Y, Yamada S, Hosokawa Y, Mariya Y, Nakamura T and Wojcik A: Serum miR-375-3p increase in mice exposed to a high dose of ionizing radiation. Sci Rep. 8:13022018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chiba M, Uehara H, Niiyama I, Kuwata H and Monzen S: Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure. Mol Med Rep 21: 2452-2458, 2020.
APA
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., & Monzen, S. (2020). Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure. Molecular Medicine Reports, 21, 2452-2458. https://doi.org/10.3892/mmr.2020.11054
MLA
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., Monzen, S."Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure". Molecular Medicine Reports 21.6 (2020): 2452-2458.
Chicago
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., Monzen, S."Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure". Molecular Medicine Reports 21, no. 6 (2020): 2452-2458. https://doi.org/10.3892/mmr.2020.11054
Copy and paste a formatted citation
x
Spandidos Publications style
Chiba M, Uehara H, Niiyama I, Kuwata H and Monzen S: Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure. Mol Med Rep 21: 2452-2458, 2020.
APA
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., & Monzen, S. (2020). Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure. Molecular Medicine Reports, 21, 2452-2458. https://doi.org/10.3892/mmr.2020.11054
MLA
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., Monzen, S."Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure". Molecular Medicine Reports 21.6 (2020): 2452-2458.
Chicago
Chiba, M., Uehara, H., Niiyama, I., Kuwata, H., Monzen, S."Changes in miRNA expressions in the injured small intestine of mice following high‑dose radiation exposure". Molecular Medicine Reports 21, no. 6 (2020): 2452-2458. https://doi.org/10.3892/mmr.2020.11054
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