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Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression

  • Authors:
    • Xiangrong Xie
    • Feng Wang
    • Linlin Zhu
    • Hongfeng Yang
    • Daorong Pan
    • Yan Liu
    • Xinliang Qu
    • Yue Gu
    • Xiaobo Li
    • Shaoliang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
    Copyright: © Xie et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2580-2588
    |
    Published online on: April 8, 2020
       https://doi.org/10.3892/mmr.2020.11060
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Abstract

Low shear stress serves an important role in the initiation and progression of atherosclerotic lesions, with an impact on progression, but its detailed mechanisms are .not yet fully known. The present study aimed to investigate endothelial cell (EC) apoptosis, as well as monocyte adhesion induced by low shear stress and the potential underlying mechanisms. The expression of platelet endothelial cell adhesion molecule‑1 (PECAM‑1) was demonstrated to be enhanced in human umbilical vascular ECs with a trend that was associated with time when stimulated by low shear stress compared with unstimulated cells. EC apoptosis was increased under low shear stress compared with unstimulated cells, and knockdown of PECAM‑1 inhibited this process. Furthermore, downregulation of PECAM‑1 reduced monocyte adhesion induced by low shear stress compared with that in the negative control cells. Mechanistically, PECAM‑1 small interfering RNA transfection increased Akt and forkhead box O1 phosphorylation under low shear stress conditions compared with that in the negative control cells. Collectively, the findings of the present study revealed that low shear stress induced EC apoptosis and monocyte adhesion by upregulating PECAM‑1 expression, which suggested that PECAM‑1 may be a potential therapeutic target for atherosclerosis.
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Copy and paste a formatted citation
Spandidos Publications style
Xie X, Wang F, Zhu L, Yang H, Pan D, Liu Y, Qu X, Gu Y, Li X, Chen S, Chen S, et al: Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression. Mol Med Rep 21: 2580-2588, 2020.
APA
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y. ... Chen, S. (2020). Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression. Molecular Medicine Reports, 21, 2580-2588. https://doi.org/10.3892/mmr.2020.11060
MLA
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y., Qu, X., Gu, Y., Li, X., Chen, S."Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression". Molecular Medicine Reports 21.6 (2020): 2580-2588.
Chicago
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y., Qu, X., Gu, Y., Li, X., Chen, S."Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression". Molecular Medicine Reports 21, no. 6 (2020): 2580-2588. https://doi.org/10.3892/mmr.2020.11060
Copy and paste a formatted citation
x
Spandidos Publications style
Xie X, Wang F, Zhu L, Yang H, Pan D, Liu Y, Qu X, Gu Y, Li X, Chen S, Chen S, et al: Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression. Mol Med Rep 21: 2580-2588, 2020.
APA
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y. ... Chen, S. (2020). Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression. Molecular Medicine Reports, 21, 2580-2588. https://doi.org/10.3892/mmr.2020.11060
MLA
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y., Qu, X., Gu, Y., Li, X., Chen, S."Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression". Molecular Medicine Reports 21.6 (2020): 2580-2588.
Chicago
Xie, X., Wang, F., Zhu, L., Yang, H., Pan, D., Liu, Y., Qu, X., Gu, Y., Li, X., Chen, S."Low shear stress induces endothelial cell apoptosis and monocyte adhesion by upregulating PECAM‑1 expression". Molecular Medicine Reports 21, no. 6 (2020): 2580-2588. https://doi.org/10.3892/mmr.2020.11060
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