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Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells

  • Authors:
    • Bo Hu
    • Xike Zhu
    • Jibin Lu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Research Center, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2553-2559
    |
    Published online on: April 10, 2020
       https://doi.org/10.3892/mmr.2020.11068
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Abstract

Cathepsin A (CTSA) is a lysosomal protease that is abnormally expressed in various types of cancer; however, the function of CTSA in lung adenocarcinoma (LUAD) is unknown. The aim of the present study was to investigate the role of CTSA during LUAD development in vitro. The Cancer Genome Atlas (TCGA) database was used to analyze the expression of CTSA mRNA in LUAD tissues. CTSA was significantly upregulated in LUAD tissues compared with normal lung tissues. To explore the effect of CTSA on LUAD in vitro, LUAD A549 cells were transfected with CTSA small interfering RNA and the hallmarks of tumorigenesis were investigated using cell proliferation, cell cycle, wound healing, invasion and western blot assays. Following CTSA knockdown, proliferation of LUAD cells was decreased and an increased proportion of LUAD cells were arrested at the G0/G1 phase, with altered expression of critical cell cycle and proliferative marker proteins, including p53, p21 and proliferating cell nuclear antigen. Moreover, CTSA knockdown decreased the migration and invasion of A549 cells, as determined by wound healing, invasion, and western blotting assays. The expression levels of key proteins involved in epithelial‑mesenchymal transition were analyzed by western blotting. CTSA knockdown enhanced the expression of E‑cadherin, but decreased the expression of N‑cadherin and β‑catenin in A549 cells. To the best of our knowledge, the present study suggested for the first time it has been identified that CTSA may serve as a tumor promoter in LUAD, enhancing the malignant progression of LUAD cells by promoting cell proliferation, migration and invasion. The results suggested that CTSA may serve as a novel therapeutic target for LUAD.
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Copy and paste a formatted citation
Spandidos Publications style
Hu B, Zhu X and Lu J: Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells. Mol Med Rep 21: 2553-2559, 2020.
APA
Hu, B., Zhu, X., & Lu, J. (2020). Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells. Molecular Medicine Reports, 21, 2553-2559. https://doi.org/10.3892/mmr.2020.11068
MLA
Hu, B., Zhu, X., Lu, J."Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells". Molecular Medicine Reports 21.6 (2020): 2553-2559.
Chicago
Hu, B., Zhu, X., Lu, J."Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells". Molecular Medicine Reports 21, no. 6 (2020): 2553-2559. https://doi.org/10.3892/mmr.2020.11068
Copy and paste a formatted citation
x
Spandidos Publications style
Hu B, Zhu X and Lu J: Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells. Mol Med Rep 21: 2553-2559, 2020.
APA
Hu, B., Zhu, X., & Lu, J. (2020). Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells. Molecular Medicine Reports, 21, 2553-2559. https://doi.org/10.3892/mmr.2020.11068
MLA
Hu, B., Zhu, X., Lu, J."Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells". Molecular Medicine Reports 21.6 (2020): 2553-2559.
Chicago
Hu, B., Zhu, X., Lu, J."Cathepsin A knockdown decreases the proliferation and invasion of A549 lung adenocarcinoma cells". Molecular Medicine Reports 21, no. 6 (2020): 2553-2559. https://doi.org/10.3892/mmr.2020.11068
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