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Article Open Access

Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway

  • Authors:
    • Zhenda Zheng
    • Min Wang
    • Cailian Cheng
    • Dinghui Liu
    • Lin Wu
    • Jieming Zhu
    • Xiaoxian Qian
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Third Affiliated Hospital of Sun Yat‑sen University, Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China, Department of Nephrology, The Third Affiliated Hospital of Sun Yat‑sen University, Sun Yat‑sen University, Guangzhou, Guangdong 510630, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 247-256
    |
    Published online on: April 28, 2020
       https://doi.org/10.3892/mmr.2020.11096
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Abstract

Endothelial dysfunction and senescence are closely associated with cardiovascular diseases including atherosclerosis and hypertension. Ginsenoside Rb1 (Rb1), the major active constituent of ginseng, has been investigated intensively because of its anti‑obesity and anti‑inflammatory effects. In a previous study, hydrogen peroxide (H2O2) was applied to induce human umbilical vein endothelial cell (HUVEC) aging. It was demonstrated that Sirtuin‑1 (SIRT1) was activated by Rb1 to protect HUVECs from H2O2‑induced senescence. However, the mechanisms are not fully understood. The present study examined the role of AMP‑activated protein kinase (AMPK), an energy sensor of cellular metabolism, in the signaling pathway of SIRT1 during H2O2‑stimulated HUVEC aging. It was identified that Rb1 restored the H2O2‑induced reduction of SIRT1 expression, which was consistent with our previous study, together with the activation of AMPK phosphorylation. Using compound C, an AMPK inhibitor, the role of AMPK in the protective effect of Rb1 against H2O2‑induced HUVEC senescence was examined. It was identified that the induction of phosphorylated AMPK by Rb1 markedly increased endothelial nitric oxide synthase expression and nitric oxide production, and suppressed PAI‑1 expression, which were abrogated in HUVECs pretreated with compound C. Further experiments demonstrated that nicotinamide, a SIRT1 inhibitor, downregulated the phosphorylation of AMPK and reduced the protective effects of Rb1 against H2O2‑induced endothelial aging. Taken together, these results provide new insights into the possible molecular mechanisms by which Rb1 protects against H2O2‑induced HUVEC senescence via the SIRT1/AMPK pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zheng Z, Wang M, Cheng C, Liu D, Wu L, Zhu J and Qian X: Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Mol Med Rep 22: 247-256, 2020.
APA
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., & Qian, X. (2020). Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Molecular Medicine Reports, 22, 247-256. https://doi.org/10.3892/mmr.2020.11096
MLA
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., Qian, X."Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway". Molecular Medicine Reports 22.1 (2020): 247-256.
Chicago
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., Qian, X."Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway". Molecular Medicine Reports 22, no. 1 (2020): 247-256. https://doi.org/10.3892/mmr.2020.11096
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng Z, Wang M, Cheng C, Liu D, Wu L, Zhu J and Qian X: Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Mol Med Rep 22: 247-256, 2020.
APA
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., & Qian, X. (2020). Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Molecular Medicine Reports, 22, 247-256. https://doi.org/10.3892/mmr.2020.11096
MLA
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., Qian, X."Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway". Molecular Medicine Reports 22.1 (2020): 247-256.
Chicago
Zheng, Z., Wang, M., Cheng, C., Liu, D., Wu, L., Zhu, J., Qian, X."Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway". Molecular Medicine Reports 22, no. 1 (2020): 247-256. https://doi.org/10.3892/mmr.2020.11096
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