Open Access

Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury

  • Authors:
    • Chunyan Wang
    • Wenlin Yuan
    • Anmin Hu
    • Juan Lin
    • Zhengyuan Xia
    • Catherine F. Yang
    • Yalan Li
    • Zhongjun Zhang
  • View Affiliations

  • Published online on: May 4, 2020     https://doi.org/10.3892/mmr.2020.11114
  • Pages: 175-184
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cardiac dysfunction resulting from sepsis may cause significant morbidity and mortality, and ferroptosis plays a role in this pathology. Dexmedetomidine (Dex), a α2‑adrenergic receptor (α2‑AR) agonist exerts cardioprotective effects against septic heart dysfunction, but the exact mechanism is unknown. In the present study, sepsis was induced by cecal ligation and puncture (CLP) in male C57BL/6 mice. Dex and yohimbine hydrochloride (YOH), an α2‑AR inhibitor, were administered before inducing CLP. Then, 24 h after CLP, serum and heart tissue were collected to detect changes of troponin‑I (TN‑I), interleukin 6 (IL‑6), superoxide dismutase (SOD), malonaldehyde (MDA) and glutathione (GSH) levels, and iron release. Ferroptosis‑targeting proteins, apoptosis and inflammatory factors were assessed by western blotting or ELISA. It was found that, 24 h after CLP, TN‑I, a biomarker of myocardial injury, was significantly increased compared with the control group. Furthermore, the levels of MDA, 8‑hydroxy‑2'‑deoxyguanosine and the inflammatory factors IL‑6 and monocyte chemoattractant protein‑1 were also significantly increased. It was demonstrated that treatment with Dex reverted or attenuated these changes (CLP + Dex vs. CLP; P<0.05), but these protective effects of Dex were reversed by YOH. Moreover, CLP significantly decreased the protein expression levels of glutathione peroxidase 4 (GPX4), SOD and GSH. However, CLP increased expression levels of heme oxygenase‑1 (HO‑1), transferrin receptor, cleaved caspase 3, inducible nitric oxide synthase and gasdermin D, and iron concentrations. It was found that Dex reversed these changes, but YOH abrogated the protective effects of Dex (CLP + Dex + YOH vs. CLP + Dex; P<0.05). Therefore, the present results suggested that the attenuation of sepsis‑induced HO‑1 overexpression and iron concentration, and the reduction of ferroptosis via enhancing GPX4, may be the major mechanisms via which Dex alleviates sepsis‑induced myocardial cellular injury.
View Figures
View References

Related Articles

Journal Cover

July-2020
Volume 22 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Yuan W, Hu A, Lin J, Xia Z, Yang C , Li Y and Zhang Z: Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury. Mol Med Rep 22: 175-184, 2020
APA
Wang, C., Yuan, W., Hu, A., Lin, J., Xia, Z., Yang, C. . ... Zhang, Z. (2020). Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury. Molecular Medicine Reports, 22, 175-184. https://doi.org/10.3892/mmr.2020.11114
MLA
Wang, C., Yuan, W., Hu, A., Lin, J., Xia, Z., Yang, C. ., Li, Y., Zhang, Z."Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury". Molecular Medicine Reports 22.1 (2020): 175-184.
Chicago
Wang, C., Yuan, W., Hu, A., Lin, J., Xia, Z., Yang, C. ., Li, Y., Zhang, Z."Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury". Molecular Medicine Reports 22, no. 1 (2020): 175-184. https://doi.org/10.3892/mmr.2020.11114