Open Access

Integrated microarray analysis to identify potential biomarkers and therapeutic targets in dilated cardiomyopathy

  • Authors:
    • Hao Zhang
    • Junyu Huo
    • Wanying Jiang
    • Qijun Shan
  • View Affiliations

  • Published online on: May 14, 2020     https://doi.org/10.3892/mmr.2020.11145
  • Pages: 915-925
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Dilated cardiomyopathy (DCM) is a primary cardiomyopathy with high mortality. The aim of the present study was to identify the related genes in DCM. The four expression profiles (GSE17800, GSE21610, GSE42955 and GSE79962) downloaded from the Gene Expression Omnibus (GEO) database were analyzed using RankProd and metaMA R packages to identify differentially expressed genes (DEGs). DEGs were uploaded to the Database for Annotation, Visualization and Integrated Discovery (DAVID), for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. A protein‑protein interaction (PPI) network of the DEGs was constructed using the STRING database. In addition, hub genes were identified using the Cytoscape plugin cytoHubba. A mouse DCM model, which established via intraperitoneal injection with doxorubicin (DOX), was used to validate the hub genes. A total of 898 DEGs were identified across the four microarrays. Furthermore, GO analysis demonstrated that these DEGs were mainly enriched in cell adhesion, negative regulation of cell proliferation, negative regulation of apoptotic process and potassium ion transport. In addition, KEGG analysis revealed that DEGs were mainly enriched in the ECM‑receptor interaction, the p53 signaling pathway, cardiac muscle contraction and the hypoxia‑inducible factor signaling pathway. Proenkephalin (PENK), chordin like 1 (CHRDL1), calumenin (CALU), apolipoprotein L1, insulin‑like growth factor binding protein 3 (IGFBP3) and ceruloplasmin (CP) were identified as hub genes in the PPI network. Furthermore, the expression levels of PENK, CHRDL1, IGFBP3, CP and CALU in hearts with DCM were validated using a mouse model. In conclusion, the present study identified six hub genes related to DCM. Therefore, the present results may provide a potential mechanism for DCM involving these hub genes, which may serve as biomarkers for screening and diagnosis in the clinic.

Related Articles

Journal Cover

August-2020
Volume 22 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Zhang, H., Huo, J., Jiang, W., & Shan, Q. (2020). Integrated microarray analysis to identify potential biomarkers and therapeutic targets in dilated cardiomyopathy. Molecular Medicine Reports, 22, 915-925. https://doi.org/10.3892/mmr.2020.11145
MLA
Zhang, H., Huo, J., Jiang, W., Shan, Q."Integrated microarray analysis to identify potential biomarkers and therapeutic targets in dilated cardiomyopathy". Molecular Medicine Reports 22.2 (2020): 915-925.
Chicago
Zhang, H., Huo, J., Jiang, W., Shan, Q."Integrated microarray analysis to identify potential biomarkers and therapeutic targets in dilated cardiomyopathy". Molecular Medicine Reports 22, no. 2 (2020): 915-925. https://doi.org/10.3892/mmr.2020.11145