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Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration

  • Authors:
    • Lu Zhang
    • I‑Ming Wang
    • Nicolas Solban
    • Razvan Cristescu
    • Gefei Zeng
    • Brian Long
  • View Affiliations / Copyright

    Affiliations: Merck and Co., Inc., Kenilworth, NJ 07033, USA
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 975-985
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    Published online on: May 22, 2020
       https://doi.org/10.3892/mmr.2020.11169
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Abstract

The MC38 (derived from carcinogen‑induced colon adenocarcinoma) tumor model is sensitive to anti‑programmed cell death‑1 (anti-PD‑1) treatment. However, there is no comprehensive description of the T and B cell receptor (TCR, BCR) repertoires of the MC38 tumor model following anti‑PD‑1 treatment, an improved understanding of which is highly important in the development of anti‑PD‑1 immunotherapy. The present study analyzed the TCR and BCR repertoires of three types of tissue, including tumor, spleen and tumor draining lymph node (DLN) from 20 MC38 syngeneic mice receiving murine anti‑PD‑1 (mDX400) treatment or mouse immunoglobulin G1 (mIgG1) control treatment. To obtain enough tissues for high‑throughput sequencing, samples were collected on day 8 after the start of initial treatment. The usage frequencies of seven TCR β chain (TRB) V genes and one TRBJ gene were significantly different between mDX400‑ and mIgG1‑group tumors. TCR repertoire diversity was significantly lower in mDX400‑group tumors compared with mIgG1‑group tumors, with the top 10 most frequent TCR clonotypes notably expanded in mDX400‑group tumors. In addition, the proportion of high‑frequency TCR clonotypes from mDX400‑group tumors that were also present both in the DLN and spleen was significantly higher than that in mIgG1‑group tumors. Among the highly expanded TCR clonotypes, one TCR clonotype was consistently expanded in >50% of the mDX400‑group tumors compared with mIgG1‑group tumors. Similarly, one BCR clonal family was highly expanded in >50% of mDX400‑group tumor samples. The consistently expanded TCR and BCR clones were co‑expanded in 29% of mDX400‑group tumors. Moreover, mutation rates of immunoglobulin heavy chain sequences in the spleen within complementarity determining region 2 and framework region 3 were significantly higher in the mDX400 group than in the mIgG1 group. The findings of this study may contribute to an improved understanding of the molecular mechanisms of anti‑PD‑1 treatment.
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View References

1 

Hamid O, Robert C, Daud A, Hodi FS, Hwu WJ, Kefford R, Wolchok JD, Hersey P, Joseph R, Weber JS, et al: Five-year survival outcomes for patients with advanced melanoma treated with pembrolizumab in KEYNOTE-001. Ann Oncol. 30:582–588. 2019. View Article : Google Scholar : PubMed/NCBI

2 

Gandhi L, Rodríguez-Abreu D, Gadgeel S, Esteban E, Felip E, De Angelis F, Domine M, Clingan P, Hochmair MJ, Powell SF, et al KEYNOTE-189 Investigators, : Pembrolizumab plus Chemotherapy in Metastatic Non-Small-Cell Lung Cancer. N Engl J Med. 378:2078–2092. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Bauml J, Seiwert TY, Pfister DG, Worden F, Liu SV, Gilbert J, Saba NF, Weiss J, Wirth L, Sukari A, et al: Pembrolizumab for platinum- and cetuximab-refractory head and neck cancer: Results from a single-arm, phase II study. J Clin Oncol. 35:1542–1549. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Sarfaty M, Hall PS, Chan KKW, Virik K, Leshno M, Gordon N, Moore A, Neiman V, Rosenbaum E and Goldstein DA: Cost-effectiveness of Pembrolizumab in Second-line Advanced Bladder Cancer. Eur Urol. 74:57–62. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Carretero-González A, Lora D, Ghanem I, Zugazagoitia J, Castellano D, Sepúlveda JM, López-Martin JA, Paz-Ares L and de Velasco G: Analysis of response rate with ANTI PD1/PD-L1 monoclonal antibodies in advanced solid tumors: A meta-analysis of randomized clinical trials. Oncotarget. 9:8706–8715. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Le DT, Durham JN, Smith KN, Wang H, Bartlett BR, Aulakh LK, Lu S, Kemberling H, Wilt C, Luber BS, et al: Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade. Science. 357:409–413. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Yarchoan M, Hopkins A and Jaffee EM: Tumor Mutational burden and response rate to PD-1 inhibition. N Engl J Med. 377:2500–2501. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Garon EB, Rizvi NA, Hui R, Leighl N, Balmanoukian AS, Eder JP, Patnaik A, Aggarwal C, Gubens M, Horn L, et al KEYNOTE-001 Investigators, : Pembrolizumab for the treatment of non-small-cell lung cancer. N Engl J Med. 372:2018–2028. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Ayers M, Lunceford J, Nebozhyn M, Murphy E, Loboda A, Kaufman DR, Albright A, Cheng JD, Kang SP, Shankaran V, et al: IFN-γ-related mRNA profile predicts clinical response to PD-1 blockade. J Clin Invest. 127:2930–2940. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Tumeh PC, Harview CL, Yearley JH, Shintaku IP, Taylor EJ, Robert L, Chmielowski B, Spasic M, Henry G, Ciobanu V, et al: PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature. 515:568–571. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Roh W, Chen P-L, Reuben A, Spencer CN, Prieto PA, Miller JP, Gopalakrishnan V, Wang F, Cooper ZA, Reddy SM, et al: Integrated molecular analysis of tumor biopsies on sequential CTLA-4 and PD-1 blockade reveals markers of response and resistance. Sci Transl Med. 9:eaah35602017. View Article : Google Scholar : PubMed/NCBI

12 

Minervina A, Pogorelyy M and Mamedov I: T-cell receptor and B-cell receptor repertoire profiling in adaptive immunity. Transpl Int. 32:1111–1123. 2019. View Article : Google Scholar : PubMed/NCBI

13 

Agata Y, Kawasaki A, Nishimura H, Ishida Y, Tsubata T, Yagita H and Honjo T: Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol. 8:765–772. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Thibult ML, Mamessier E, Gertner-Dardenne J, Pastor S, Just-Landi S, Xerri L, Chetaille B and Olive D: PD-1 is a novel regulator of human B-cell activation. Int Immunol. 25:129–137. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Hollern DP, Xu N, Thennavan A, Glodowski C, Garcia-Recio S, Mott KR, He X, Garay JP, Carey-Ewend K, Marron D, et al: B cells and T follicular helper cells mediate response to checkpoint inhibitors in high mutation burden mouse models of breast cancer. Cell. 179:1191–1206.e21. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Selitsky SR, Mose LE, Smith CC, Chai S, Hoadley KA, Dittmer DP, Moschos SJ, Parker JS and Vincent BG: Prognostic value of B cells in cutaneous melanoma. Genome Med. 11:362019. View Article : Google Scholar : PubMed/NCBI

17 

Helmink BA, Reddy SM, Gao J, Zhang S, Basar R, Thakur R, Yizhak K, Sade-Feldman M, Blando J, Han G, et al: B cells and tertiary lymphoid structures promote immunotherapy response. Nature. 577:549–555. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Olson B, Li Y, Lin Y, Liu ET and Patnaik A: Mouse Models for cancer immunotherapy research. Cancer Discov. 8:1358–1365. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Hossain DMS, Javaid S, Cai M, Zhang C, Sawant A, Hinton M, Sathe M, Grein J, Blumenschein W, Pinheiro EM, et al: Dinaciclib induces immunogenic cell death and enhances anti-PD1-mediated tumor suppression. J Clin Invest. 128:644–654. 2018. View Article : Google Scholar : PubMed/NCBI

20 

Ngiow SF, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvogel L and Smyth MJ: A threshold level of intratumor CD8+ T-cell PD1 expression dictates therapeutic response to anti-PD1. Cancer Res. 75:3800–3811. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Homet Moreno B, Zaretsky JM, Garcia-Diaz A, Tsoi J, Parisi G, Robert L, Meeth K, Ndoye A, Bosenberg M, Weeraratna AT, et al: Response to Programmed cell death-1 blockade in a murine melanoma syngeneic model requires costimulation, CD4, and CD8 T Cells. Cancer Immunol Res. 4:845–857. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Grasselly C, Denis M, Bourguignon A, Talhi N, Mathe D, Tourette A, Serre L, Jordheim LP, Matera EL and Dumontet C: The Antitumor activity of combinations of cytotoxic chemotherapy and immune checkpoint inhibitors is model-dependent. Front Immunol. 9:21002018. View Article : Google Scholar : PubMed/NCBI

23 

Efremova M, Rieder D, Klepsch V, Charoentong P, Finotello F, Hackl H, Hermann-Kleiter N, Löwer M, Baier G, Krogsdam A, et al: Targeting immune checkpoints potentiates immunoediting and changes the dynamics of tumor evolution. Nat Commun. 9:322018. View Article : Google Scholar : PubMed/NCBI

24 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals, . Guide for the Care and Use of Laboratory Animals. (8th). National Academies Press. (Washington, DC). 2011.

25 

Masella AP, Bartram AK, Truszkowski JM, Brown DG and Neufeld JD: PANDAseq: Paired-end assembler for illumina sequences. BMC Bioinformatics. 13:312012. View Article : Google Scholar : PubMed/NCBI

26 

Giudicelli V, Chaume D and Lefranc MP: IMGT/GENE-DB: A comprehensive database for human and mouse immunoglobulin and T cell receptor genes. Nucleic Acids Res. 33:D256–D261. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Ye J, Ma N, Madden TL and Ostell JM: IgBLAST: An immunoglobulin variable domain sequence analysis tool. Nucleic Acids Res. 41((W1)): W34–40. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Gupta NT, Vander Heiden JA, Uduman M, Gadala-Maria D, Yaari G and Kleinstein SH: Change-O: A toolkit for analyzing large-scale B cell immunoglobulin repertoire sequencing data. Bioinformatics. 31:3356–3358. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Hill MO: Diversity and Evenness: A unifying notation and its consequences. Ecology. 54:427–432. 1973. View Article : Google Scholar

30 

Shugay M, Bagaev DV, Zvyagin IV, Vroomans RM, Crawford JC, Dolton G, Komech EA, Sycheva AL, Koneva AE, Egorov ES, et al: VDJdb: A curated database of T-cell receptor sequences with known antigen specificity. Nucleic Acids Res. 46(D1): D419–D427. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Tickotsky N, Sagiv T, Prilusky J, Shifrut E and Friedman N: McPAS-TCR: A manually curated catalogue of pathology-associated T cell receptor sequences. Bioinformatics. 33:2924–2929. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Gros A, Robbins PF, Yao X, Li YF, Turcotte S, Tran E, Wunderlich JR, Mixon A, Farid S, Dudley ME, et al: PD-1 identifies the patient-specific CD8+ tumor-reactive repertoire infiltrating human tumors. J Clin Invest. 124:2246–2259. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Pasetto A, Gros A, Robbins PF, Deniger DC, Prickett TD, Matus-Nicodemos R, Douek DC, Howie B, Robins H, Parkhurst MR, et al: Tumor- and Neoantigen-reactive T-cell receptors can be identified based on their frequency in fresh tumor. Cancer Immunol Res. 4:734–743. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Hosoi A, Takeda K, Nagaoka K, Iino T, Matsushita H, Ueha S, Aoki S, Matsushima K, Kubo M, Morikawa T, et al: Increased diversity with reduced “diversity evenness” of tumor infiltrating T-cells for the successful cancer immunotherapy. Sci Rep. 8:10582018. View Article : Google Scholar : PubMed/NCBI

35 

Lehmann-Horn K, Wang SZ, Sagan SA, Zamvil SS and von Büdingen HC: B cell repertoire expansion occurs in meningeal ectopic lymphoid tissue. JCI Insight. 1:e872342016. View Article : Google Scholar : PubMed/NCBI

36 

Katoh H, Komura D, Konishi H, Suzuki R, Yamamoto A, Kakiuchi M, Sato R, Ushiku T, Yamamoto S, Tatsuno K, et al: Immunogenetic profiling for gastric cancers identifies sulfated glycosaminoglycans as major and functional b cell antigens in human malignancies. Cell Rep. 20:1073–1087. 2017. View Article : Google Scholar : PubMed/NCBI

37 

DeFalco J, Harbell M, Manning-Bog A, Baia G, Scholz A, Millare B, Sumi M, Zhang D, Chu F, Dowd C, et al: Non-progressing cancer patients have persistent B cell responses expressing shared antibody paratopes that target public tumor antigens. Clin Immunol. 187:37–45. 2018. View Article : Google Scholar : PubMed/NCBI

38 

Probst P, Stringhini M and Neri D: Immunotherapy of CT26 murine tumors is characterized by an oligoclonal response against the AH1 tumor rejection antigen. bioRxiv. doi.org/10.1101/789784.

39 

Rock EP, Sibbald PR, Davis MM and Chien YH: CDR3 length in antigen-specific immune receptors. J Exp Med. 179:323–328. 1994. View Article : Google Scholar : PubMed/NCBI

40 

John LB, Devaud C, Duong CP, Yong CS, Beavis PA, Haynes NM, Chow MT, Smyth MJ, Kershaw MH and Darcy PK: Anti-PD-1 antibody therapy potently enhances the eradication of established tumors by gene-modified T cells. Clin Cancer Res. 19:5636–5646. 2013. View Article : Google Scholar : PubMed/NCBI

41 

Chung V, Kos FJ, Hardwick N, Yuan Y, Chao J, Li D, Waisman J, Li M, Zurcher K, Frankel P, et al: Evaluation of safety and efficacy of p53MVA vaccine combined with pembrolizumab in patients with advanced solid cancers. Clin Transl Oncol. 21:363–372. 2019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Wang IM, Solban N, Cristescu R, Zeng G and Long B: Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration. Mol Med Rep 22: 975-985, 2020.
APA
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., & Long, B. (2020). Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration. Molecular Medicine Reports, 22, 975-985. https://doi.org/10.3892/mmr.2020.11169
MLA
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., Long, B."Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration". Molecular Medicine Reports 22.2 (2020): 975-985.
Chicago
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., Long, B."Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration". Molecular Medicine Reports 22, no. 2 (2020): 975-985. https://doi.org/10.3892/mmr.2020.11169
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Wang IM, Solban N, Cristescu R, Zeng G and Long B: Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration. Mol Med Rep 22: 975-985, 2020.
APA
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., & Long, B. (2020). Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration. Molecular Medicine Reports, 22, 975-985. https://doi.org/10.3892/mmr.2020.11169
MLA
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., Long, B."Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration". Molecular Medicine Reports 22.2 (2020): 975-985.
Chicago
Zhang, L., Wang, I., Solban, N., Cristescu, R., Zeng, G., Long, B."Comprehensive investigation of T and B cell receptor repertoires in an MC38 tumor model following murine anti‑PD‑1 administration". Molecular Medicine Reports 22, no. 2 (2020): 975-985. https://doi.org/10.3892/mmr.2020.11169
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