Effects of an ethyl acetate extract of Daphne altaica stem bark on the cell cycle, apoptosis and expression of PPARγ in Eca‑109 human esophageal carcinoma cells

Kizaibek,Murat; Wubuli,Ayixiamuguli; Gu,Zhengbing; Bahetjan,Didar; Tursinbai,Lazzat; Nurhamit,Kamishbek; Chen,Bin; Wang,Jing; Tahan,Omirshat; Cao,Peng

doi:10.3892/mmr.2020.11187

Effects of an ethyl acetate extract of Daphne altaica stem bark on the cell cycle, apoptosis and expression of PPARγ in Eca‑109 human esophageal carcinoma cells

Authors:
- Murat Kizaibek
- Ayixiamuguli Wubuli
- Zhengbing Gu
- Didar Bahetjan
- Lazzat Tursinbai
- Kamishbek Nurhamit
- Bin Chen
- Jing Wang
- Omirshat Tahan
- Peng Cao
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Affiliations: Traditional Chinese Medicine Hospital of Ili Kazakh Autonomous Prefecture, Yining, Xinjiang 835000, P.R. China, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Science, Urumqi, Xinjiang 830011, P.R. China, Jiangsu Yongjian Medical Technology Ltd. Co., Taizhou, Jiangsu 225300, P.R. China, Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing, Jiangsu 210042, P.R. China, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210028, P.R. China, College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi, Xinjiang 830052, P.R. China
Published online on: May 27, 2020 https://doi.org/10.3892/mmr.2020.11187
Pages: 1400-1408
Copyright: © Kizaibek et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Daphne altaica Pall. (D. altaica; Thymelaeaceae) has long been used in traditional Kazakh medicine for the treatment of cancer and respiratory diseases. Previous studies have demonstrated the in vitro anticancer effects of D. altaica extract and its constituents in certain cancer cell lines; however, the underlying molecular mechanisms are not completely understooD. The present study aimed to investigate the molecular mechanisms underlying the activity of an ethyl acetate extract of D. altaica (Da‑Ea) by assessing its effects on cell morphology, cell apoptosis, cell cycle progression and the expression levels of peroxisome proliferator‑activated receptor γ (PPARγ) in Eca‑109 cells. Cell morphology was observed under a phase contrast microscope. Cell apoptosis and cell cycle progression were assessed by flow cytometry following Annexin V/propidium iodide (PI) double staining and PI single staining, respectively. The mRNA and protein expression levels of PPARγ were determined by reverse transcription‑quantitative PCR and western blotting, respectively. Compared with the control group, the percentage of apoptotic cells, cell cycle arrest at S phase and apoptotic morphological cell characteristics were increased in Da‑Ea‑treated Eca‑109 cells. Furthermore, Da‑Ea treatment upregulated the mRNA and protein expression levels of PPARγ compared with the control cells. High‑performance liquid chromatography with diode‑array detection indicated that daphnetin‑7‑O‑β‑D‑glucoside, daphnetin, demethyldaphnoretin‑7‑O‑β‑D‑glucopyranoside and genkwanol A were the main constituents of Da‑Ea. Collectively, the results suggested that Da‑Ea displayed antiproliferative activities in Eca‑109 cells by inducing apoptosis and S phase cell cycle arrest, as well as upregulating PPARγ expression levels.

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