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Article Open Access

Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1

Retraction in: /10.3892/mmr.2025.13718
  • Authors:
    • Feng Zhou
    • Chunling Du
    • Donghui Xu
    • Jinchang Lu
    • Lei Zhou
    • Chaomin Wu
    • Bo Wu
    • Jianan Huang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Respiratory Medicine, QingPu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai 201700, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1382-1390
    |
    Published online on: May 27, 2020
       https://doi.org/10.3892/mmr.2020.11188
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Abstract

Lung cancer is a devastating cancer with high morbidity and mortality. Ubiquitin‑specific protease (USP) is a type of deubiquitinating enzyme (DUB) that has been implicated in numerous cancers, including colorectal, myeloma and breast. In the present study, the expression of USP51 was determined in the lung cancer cell line A549 and cisplatin (also known as DDP)‑resistant lung cancer strain A549/DDP. The expression of zinc‑finger E‑box binding homeobox 1 (ZEB1), a transcriptional repressor, was also examined. The effects of USP51 knockdown or overexpression on proliferation and apoptosis, as well as the impact of ZEB1 overexpression and USP51 interference on apoptosis and ubiquitination were then assessed. Notably, increased expression of USP51 and ZEB1 in A549/DDP cells was observed, and treatment with DDP significantly inhibited proliferation in A549/DDP cells. In addition, knockdown of USP51 in A549/DDP cells significantly induced apoptosis, decreased ZEB1 expression and increased cleaved poly ADP‑ribose polymerase 1 (PARP1) and cleaved caspase‑3 levels. Consistently, USP51 overexpression in A549 cells displayed the opposite effects and potently attenuated DDP‑induced apoptosis. Notably, overexpression of ZEB1 in A549/DDP cells potently attenuated the effects of USP51 knockdown on apoptosis, and co‑IP experiments further demonstrated interaction between USP51 and ZEB. Lastly, knockdown of USP51 promoted ZEB1 ubiquitination, leading to ZEB1 degradation. Collectively, the present findings demonstrated that USP51 inhibition attenuated DDP resistance in A549/DDP cells via ubiquitin‑mediated degradation of ZEB1. Hence, targeting USP51 may serve as a novel therapeutic target for DDP resistance in lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou F, Du C, Xu D, Lu J, Zhou L, Wu C, Wu B and Huang J: Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718. Mol Med Rep 22: 1382-1390, 2020.
APA
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C. ... Huang, J. (2020). Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718. Molecular Medicine Reports, 22, 1382-1390. https://doi.org/10.3892/mmr.2020.11188
MLA
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C., Wu, B., Huang, J."Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718". Molecular Medicine Reports 22.2 (2020): 1382-1390.
Chicago
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C., Wu, B., Huang, J."Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718". Molecular Medicine Reports 22, no. 2 (2020): 1382-1390. https://doi.org/10.3892/mmr.2020.11188
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou F, Du C, Xu D, Lu J, Zhou L, Wu C, Wu B and Huang J: Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718. Mol Med Rep 22: 1382-1390, 2020.
APA
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C. ... Huang, J. (2020). Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718. Molecular Medicine Reports, 22, 1382-1390. https://doi.org/10.3892/mmr.2020.11188
MLA
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C., Wu, B., Huang, J."Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718". Molecular Medicine Reports 22.2 (2020): 1382-1390.
Chicago
Zhou, F., Du, C., Xu, D., Lu, J., Zhou, L., Wu, C., Wu, B., Huang, J."Knockdown of ubiquitin‑specific protease 51 attenuates cisplatin resistance in lung cancer through ubiquitination of zinc‑finger E‑box binding homeobox 1 Retraction in /10.3892/mmr.2025.13718". Molecular Medicine Reports 22, no. 2 (2020): 1382-1390. https://doi.org/10.3892/mmr.2020.11188
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