Open Access

Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint

  • Authors:
    • Jiaxin Jia
    • Jiadong Sun
    • Wenting Liao
    • Lingling Qin
    • Kai Su
    • Yiqing He
    • Jiaqiang Zhang
    • Ronchung Yang
    • Zhiguang Zhang
    • Yangpeng Sun
  • View Affiliations

  • Published online on: May 28, 2020     https://doi.org/10.3892/mmr.2020.11193
  • Pages: 1195-1204
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Interleukin (IL)‑1β is a key promotor in the pathogenesis of temporomandibular joint osteoarthritis. Differentiation of stem cells to cartilage is a crucial repair mechanism of articular cartilage damage, and IL‑1β has been reported to impede the differentiation by upregulating the secretion of IL‑6, an important inflammatory factor. Long non‑coding RNAs (lncRNAs) regulate a number of physiological and pathological processes, but whether lncRNA AK094629 contributes to the IL‑1β mediated induction of inflammation remains unclear. Therefore, the aim of the present study was to investigate the effect of AK094629 on IL‑1β‑induced IL‑6 expression in synovial‑derived mesenchymal stem cells (SMSCs) of the temporomandibular joints. The results of the present study demonstrated that the expression of AK094629 in the synovial tissue of patients with osteoarthritis was positively correlated with IL‑1β. In addition, IL‑1β upregulated the expression of AK094629 in the SMSCs in vitro, and AK094629 knockdown inhibited the IL‑1β mediated upregulation of IL‑6. The present study also demonstrated that AK094629 knockdown downregulated the expression of the mitogen‑activated protein kinase kinase kinase 4 (MAP3K4), which is upregulated by IL‑1β, whereas knockdown of MAP3K4 did not affect the expression of AK094629, but reversed the upregulation of IL‑6 in SMSCs. In conclusion, AK094629 knockdown attenuated the expression of IL‑1β‑regulated IL‑6 in the SMSCs of the temporomandibular joint by inhibiting MAP3K4. Therefore, AK094629 may be a potential novel therapeutic target for the treatment of temporomandibular joint osteoarthritis.

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August-2020
Volume 22 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos
Jia J, Sun J, Liao W, Qin L, Su K, He Y, et al: Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint. Mol Med Rep 22: 1195-1204, 2020
APA
Jia, J., Sun, J., Liao, W., Qin, L., Su, K., He, Y. ... Sun, Y. (2020). Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint. Molecular Medicine Reports, 22, 1195-1204. https://doi.org/10.3892/mmr.2020.11193
MLA
Jia, J., Sun, J., Liao, W., Qin, L., Su, K., He, Y., Zhang, J., Yang, R., Zhang, Z., Sun, Y."Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint". Molecular Medicine Reports 22.2 (2020): 1195-1204.
Chicago
Jia, J., Sun, J., Liao, W., Qin, L., Su, K., He, Y., Zhang, J., Yang, R., Zhang, Z., Sun, Y."Knockdown of long non‑coding RNA AK094629 attenuates the interleukin‑1β induced expression of interleukin‑6 in synovium‑derived mesenchymal stem cells from the temporomandibular joint". Molecular Medicine Reports 22, no. 2 (2020): 1195-1204. https://doi.org/10.3892/mmr.2020.11193