Open Access

Cytoprotective role of humanin in lens epithelial cell oxidative stress‑induced injury

  • Authors:
    • Hao Yang
    • Yilei Cui
    • Yelei Tang
    • Xiajing Tang
    • Xiaoning Yu
    • Jiayue Zhou
    • Qichuan Yin
    • Xingchao Shentu
  • View Affiliations

  • Published online on: June 2, 2020     https://doi.org/10.3892/mmr.2020.11202
  • Pages: 1467-1479
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxidative stress-induced injury and apoptosis of human lens epithelial cells (HLECs) are early events in the development of age‑related cataracts (ARCs). Humanin (HN) is a mitochondrial‑related peptide that serves a cytoprotective role in various cell types and animal models. Following HN knockdown or overexpression, the level of reactive oxygen species (ROS), mitochondrial membrane potential and mitochondrial DNA copy number, cell viability, LDH activity and apoptosis of HLECs under oxidative stress were detected, and apoptosis and autophagy were detected via transmission electron microscopy. The results suggested that HN may be involved in the response of HLECs to oxidative stress, and that HN expression was significantly upregulated under oxidative stress conditions. Furthermore, exogenous HN reduced intracellular ROS content and mitochondrial damage, and enhanced mitochondrial biosynthesis; however, this protection was lost in an endogenous HN knockdown cell model. In addition, to the best of our knowledge, the present study was the first to identify that HN increased mitochondrial autophagy, which was involved in reducing ROS production under oxidative stress. The present study indicated a potential mechanism underlying the anti‑oxidative damage and apoptotic effects of HN under oxidative stress. In conclusion, HN may be a potential therapeutic target for ARCs as it has a significant cellular protective effect on HLECs under oxidative stress; therefore, further study is required to investigate its role in the occurrence and development of ARCs.

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August-2020
Volume 22 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Yang, H., Cui, Y., Tang, Y., Tang, X., Yu, X., Zhou, J. ... Shentu, X. (2020). Cytoprotective role of humanin in lens epithelial cell oxidative stress‑induced injury. Molecular Medicine Reports, 22, 1467-1479. https://doi.org/10.3892/mmr.2020.11202
MLA
Yang, H., Cui, Y., Tang, Y., Tang, X., Yu, X., Zhou, J., Yin, Q., Shentu, X."Cytoprotective role of humanin in lens epithelial cell oxidative stress‑induced injury". Molecular Medicine Reports 22.2 (2020): 1467-1479.
Chicago
Yang, H., Cui, Y., Tang, Y., Tang, X., Yu, X., Zhou, J., Yin, Q., Shentu, X."Cytoprotective role of humanin in lens epithelial cell oxidative stress‑induced injury". Molecular Medicine Reports 22, no. 2 (2020): 1467-1479. https://doi.org/10.3892/mmr.2020.11202