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Article

Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling

  • Authors:
    • Gangming Xu
    • Manyun Dai
    • Xiuting Zheng
    • Hante Lin
    • Aiming Liu
    • Julin Yang
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Medical School of Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Basic Nutrition, Ningbo College of Health Sciences, Ningbo, Zhejiang 315100, P.R. China
  • Pages: 1583-1593
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    Published online on: June 3, 2020
       https://doi.org/10.3892/mmr.2020.11210
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Abstract

α‑naphthylisothiocyanate (ANIT) is used to induce intrahepatic cholestasis and it is frequently used for investigations into the disease mechanism. The lithocholic acid (LCA) cholestatic model has also been extensively used in various studies; however, to the best of our knowledge, a comparative study determining the hepatotoxic mechanisms induced by these two models has not been previously conducted. In the present study, ICR mice were treated with ANIT or LCA to induce cholestatic liver injury. Biochemical analysis was used to determine the serum. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bile acid (TBA) levels, and histopathological assessment was used to examine the liver tissue. Metabolomic analysis was used for the serum biomarker identification. Reverse transcription‑quantitative PCR analysis and western blotting were used to analyze the inflammation biomarkers. The serum metabolome of the ANIT group clustered away from of the LCA group, which was demonstrated by the different modifications of the BA components. ALP level was found to be preferentially increased in the ANIT group from 24 to 48 h. Total BA levels was only increased in the ANIT group at 24 h. In contrast, AST and ALT activity levels were preferentially increased in the LCA group. The bile ducts in the hepatic tissues of the ANIT group were observed to be severely dilated, whereas the presence of edematous hepatocytes around the necrotic lesions and neutrophil infiltration were identified in the LCA group. The expression levels of cholesterol 7α‑hydroxylase and sterol 12α‑hydroxylase genes were significantly downregulated in the ANIT group compared with the LCA group, where a stronger adaptation of BA metabolism was supported by major differences in the concentration of the BA components. Despite the aforementioned etiological differences in the cholestasis induced by each treatment, the activation of the JNK/STAT3 signaling pathway was similar between the two cholestatic models. In conclusion, these data suggested that the liver injury induced by ANIT may be cholestatic, while the liver injury caused in the LCA model may be hepatocellular. Moreover, the downstream cholestatic liver injury in both models was indicated to be mediated by the JNK/STAT3 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xu G, Dai M, Zheng X, Lin H, Liu A and Yang J: Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling . Mol Med Rep 22: 1583-1593, 2020.
APA
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., & Yang, J. (2020). Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling . Molecular Medicine Reports, 22, 1583-1593. https://doi.org/10.3892/mmr.2020.11210
MLA
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., Yang, J."Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling ". Molecular Medicine Reports 22.2 (2020): 1583-1593.
Chicago
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., Yang, J."Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling ". Molecular Medicine Reports 22, no. 2 (2020): 1583-1593. https://doi.org/10.3892/mmr.2020.11210
Copy and paste a formatted citation
x
Spandidos Publications style
Xu G, Dai M, Zheng X, Lin H, Liu A and Yang J: Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling . Mol Med Rep 22: 1583-1593, 2020.
APA
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., & Yang, J. (2020). Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling . Molecular Medicine Reports, 22, 1583-1593. https://doi.org/10.3892/mmr.2020.11210
MLA
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., Yang, J."Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling ". Molecular Medicine Reports 22.2 (2020): 1583-1593.
Chicago
Xu, G., Dai, M., Zheng, X., Lin, H., Liu, A., Yang, J."Cholestatic models induced by lithocholic acid and α‑naphthylisothiocyanate: Different etiological mechanisms for liver injury but shared JNK/STAT3 signaling ". Molecular Medicine Reports 22, no. 2 (2020): 1583-1593. https://doi.org/10.3892/mmr.2020.11210
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