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Article Open Access

Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways

  • Authors:
    • Li‑Juan Cao
    • Hai‑Tang Xie
    • Zhong‑Xia Chu
    • Yue Ma
    • Ming‑Ming Wang
    • Zhuang Shi
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region 028007, P.R. China, Department of Mongolian Medicine Hand Foot Surgery, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region 028007, P.R. China
    Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1527-1535
    |
    Published online on: June 11, 2020
       https://doi.org/10.3892/mmr.2020.11224
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Abstract

Tubeimoside-1 (TBMS1), a traditional Chinese herb extracted from Bolbostemma paniculatum (Maxim.), induces apoptosis in a number of human cancer cell lines. TBMS1 has been reported to induce apoptosis in human glioma cells, however the mechanism remains to be elucidated. The present study explored TBMS1‑induced PI3K/Akt‑related pathways in human glioma cells. The human glioma U251 and the human astrocyte (HA) cell lines were treated with various concentrations of TBMS1. MTT assays were conducted to analyze cell viability. Cell cycle distribution and the rate of apoptosis were assessed using flow cytometry. BrdU incorporation and Hoechst 33342 staining were performed to analyze the cell cycle and apoptosis, respectively. Western blotting was performed to investigate protein expression levels. The results demonstrated that TBMS1 reduced cell viability in human glioma cells U251 by suppressing Akt phosphorylation. Subsequently, TBMS1 inhibited DNA synthesis and induced G2/M phase arrest by targeting the PI3K/Akt/p21 and the cyclin‑dependent kinase 1/cyclin B1 signaling cascades. In addition, TBMS1 triggered apoptosis via the PI3K/Akt‑mediated Bcl‑2 signaling pathway. These results demonstrated that TBMS1 prevented the progression of gliomas via the PI3K/Akt‑dependent pathway, which provided a theoretical basis for in vivo studies to use TBMS1 as potential therapy for the prevention of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Cao LJ, Xie HT, Chu ZX, Ma Y, Wang MM and Shi Z: Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways. Mol Med Rep 22: 1527-1535, 2020.
APA
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., & Shi, Z. (2020). Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways. Molecular Medicine Reports, 22, 1527-1535. https://doi.org/10.3892/mmr.2020.11224
MLA
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., Shi, Z."Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways". Molecular Medicine Reports 22.2 (2020): 1527-1535.
Chicago
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., Shi, Z."Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways". Molecular Medicine Reports 22, no. 2 (2020): 1527-1535. https://doi.org/10.3892/mmr.2020.11224
Copy and paste a formatted citation
x
Spandidos Publications style
Cao LJ, Xie HT, Chu ZX, Ma Y, Wang MM and Shi Z: Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways. Mol Med Rep 22: 1527-1535, 2020.
APA
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., & Shi, Z. (2020). Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways. Molecular Medicine Reports, 22, 1527-1535. https://doi.org/10.3892/mmr.2020.11224
MLA
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., Shi, Z."Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways". Molecular Medicine Reports 22.2 (2020): 1527-1535.
Chicago
Cao, L., Xie, H., Chu, Z., Ma, Y., Wang, M., Shi, Z."Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways". Molecular Medicine Reports 22, no. 2 (2020): 1527-1535. https://doi.org/10.3892/mmr.2020.11224
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