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Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells

  • Authors:
    • Xiaopeng Shen
    • Shen Wu
    • Jingyi Zhang
    • Meng Li
    • Feng Xu
    • Ao Wang
    • Yang Lei
    • Guoping Zhu
  • View Affiliations / Copyright

    Affiliations: Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, P.R. China, Department of Inspection, Wuhu Center for Disease Control and Prevention, Wuhu, Anhui 241000, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1949-1957
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    Published online on: June 18, 2020
       https://doi.org/10.3892/mmr.2020.11250
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Abstract

Glioblastoma (GBM) is the most common type of brain cancer and has the highest mortality. Dysregulated expression of wild‑type isocitrate dehydrogenase 1 (IDH1) has been demonstrated to promote the progression of primary GBM without accumulating D‑2‑hydroxyglutarate, which differs from IDH1 mutation‑related mechanisms of tumorigenesis. Previous studies have revealed several roles of wild‑type IDH1 in primary GBM, involving proliferation and apoptosis. However, the function of IDH1 in cell migration has not been investigated. In the current study, the results of bioinformatics analysis revealed that IDH1 expression was significantly upregulated in patients with primary GBM. Wound healing and Transwell assays demonstrated that IDH1 overexpression promoted cell migration in primary GBM cells and that IDH1 knockdown hindered this process. Furthermore, α‑ketoglutarate (α‑KG), which is the main product of IDH1‑catalyzed reactions, was significantly decreased by IDH1 knockdown and upregulated by IDH1 overexpression. α‑KG treatment significantly increased the migration of primary GBM cells. Additionally, RNA sequence analysis of patients with primary GBM reported significant alterations in the expression of phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway‑regulated genes, including Myc, Snail family transcriptional repressor 2 and Twist‑related protein 1, which are primarily cell migration regulatory factors. Western blotting revealed that the overexpression or knockdown of IDH1 promoted or inhibited the PI3K/AKT/mTOR pathway, respectively. α‑KG treatment of primary GBM cells also promoted the PI3K/AKT/mTOR pathway. Furthermore, IDH1‑overexpressing and α‑KG‑treated U87 cells were incubated with rapamycin, an mTOR‑specific inhibitor, and the results revealed that rapamycin treatment reversed the increased cell migration caused by IDH1 overexpression and α‑KG treatment. The results indicated that IDH1 regulated the migration of primary GBM cells by altering α‑KG levels and that the function of the IDH1/α‑KG axis may rely on PI3K/AKT/mTOR pathway regulation.
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View References

1 

Mamelak AN and Jacoby DB: Targeted delivery of antitumoral therapy to glioma and other malignancies with synthetic chlorotoxin (TM-601). Expert Opin Drug Deliv. 4:175–186. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Yang K, Niu L, Bai Y and Le W: Glioblastoma: Targeting the autophagy in tumorigenesis. Brain Res Bull. 153:334–340. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Gallego O: Nonsurgical treatment of recurrent glioblastoma. Curr Oncol. 22:e273–e281. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Bergaggio E and Piva R: Wild-type IDH enzymes as actionable targets for cancer therapy. Cancers (Basel). 11:5632019. View Article : Google Scholar

5 

Kirkman HN, Galiano S and Gaetani GF: The function of catalase-bound NADPH. J Biol Chem. 262:660–666. 1987.PubMed/NCBI

6 

Itsumi M, Inoue S, Elia AJ, Murakami K, Sasaki M, Lind EF, Brenner D, Harris IS, Chio IIC, Afzal S, et al: Idh1 protects murine hepatocytes from endotoxin-induced oxidative stress by regulating the intracellular NADP(+)/NADPH ratio. Cell Death Differ. 22:1837–1845. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Lee SM, Koh HJ, Park DC, Song BJ, Huh TL and Park JW: Cytosolic NADP(+)-dependent isocitrate dehydrogenase status modulates oxidative damage to cells. Free Radic Biol Med. 32:1185–1196. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Gagné LM, Boulay K, Topisirovic I, Huot ME and Mallette FA: Oncogenic activities of IDH1/2 mutations: From epigenetics to cellular signaling. Trends Cell Biol. 27:738–752. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Pansuriya TC, van Eijk R, d'Adamo P, van Ruler M, Kuijjer ML, Oosting J, Cleton-Jansen AM, van Oosterwijk JG, Verbeke SLF, Meijer D, et al: Somatic mosaic IDH1 and IDH2 mutations are associated with enchondroma and spindle cell hemangioma in ollier disease and maffucci syndrome. Nat Genet. 43:1256–1261. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Pansuriya TC, Kroon HM and Bovee JV: Enchondromatosis: Insights on the different subtypes. Int J Clin Exp Pathol. 3:557–569. 2010.PubMed/NCBI

11 

Struys EA, Salomons GS, Achouri Y, Van Schaftingen E, Grosso S, Craigen WJ, Verhoeven NM and Jakobs C: Mutations in the D-2-hydroxyglutarate dehydrogenase gene cause D-2-hydroxyglutaric aciduria. Am J Hum Genet. 76:358–360. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Parsons DW, Jones S, Zhang X, Lin JCH, Leary RJ, Angenendt P, Mankoo P, Carter H, Siu IM, Gallia GL, et al: An integrated genomic analysis of human glioblastoma multiforme. Science. 321:1807–1812. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Yan H, Parsons DW, Jin G, McLendon R, Rasheed BA, Yuan W, Kos I, Haberle IB, Jones S, Riggins GJ, et al: IDH1 and IDH2 mutations in gliomas. N Engl J Med. 360:765–773. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Balss J, Meyer J, Mueller W, Korshunov A, Hartmann C and von Deimling A: Analysis of the IDH1 codon 132 mutation in brain tumors. Acta Neuropathol. 116:597–602. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Calvert AE, Chalastanis A, Wu Y, Hurley LA, Kouri FM, Bi Y, Kachman M, May JL, Bartom E, Hua Y, et al: Cancer-associated IDH1 promotes growth and resistance to targeted therapies in the absence of mutation. Cell Rep. 19:1858–1873. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Ma QL, Wang JH, Wang YG, Hu C, Mu QT, Yu MX, Wang L, Wang DM, Yang M, Yin XF, et al: High IDH1 expression is associated with a poor prognosis in cytogenetically normal acute myeloid leukemia. Int J Cancer. 137:1058–1065. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Robbins D, Wittwer JA, Codarin S, Circu ML, Aw TY, Huang TT, Van Remmen H, Richardson A, Wang DB, Witt SN, et al: Isocitrate dehydrogenase 1 is downregulated during early skin tumorigenesis which can be inhibited by overexpression of manganese superoxide dismutase. Cancer Sci. 103:1429–1433. 2012. View Article : Google Scholar : PubMed/NCBI

18 

DiNardo CD, Jabbour E, Ravandi F, Takahashi K, Daver N, Routbort M, Patel KP, Brandt M, Pierce S, Kantarjian H and Manero GG: IDH1 and IDH2 mutations in myelodysplastic syndromes and role in disease progression. Leukemia. 30:980–984. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC, et al: Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature. 462:739–744. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Wahab OA, Edwards CR, Khanin R, Figueroa ME, Melnick A, et al: IDH mutation impairs histone demethylation and results in a block to cell differentiation. Nature. 483:474–478. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Xu W, Yang H, Liu Y, Yang Y, Wang P, Kim SH, Ito S, Yang C, Wang P, Xiao NT, et al: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of alpha-ketoglutarate-dependent dioxygenases. Cancer Cell. 19:17–30. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Tan F, Jiang Y, Sun N, Chen Z, Lv Y, Shao K, Li N, Qiu B, Gao Y, Li B, et al: Identification of isocitrate dehydrogenase 1 as a potential diagnostic and prognostic biomarker for non-small cell lung cancer by proteomic analysis. Mol Cell Proteomics. 11:M111 008821. 2012.

23 

Reid Y, Storts D, Riss T and Minor L: Authentication of human cell lines by STR DNA profiling analysis. Assay Guidance Manual (Internet) Bethesda (MD): Sittampalam GS, Grossman A, Brimacombe K, Arkin M, Auld D, Austin CP, Baell J, Bejcek B, Caaveiro JMM, Chung TDY, et al: Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2013, May 1–2013

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

van de Merbel AF, van der Horst G, Buijs JT and van der Pluijm G: Protocols for migration and invasion studies in prostate cancer. Methods Mol Biol. 1786:67–79. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Chandrashekar DS, Bashel B, Balasubramanya SAH, Creighton CJ, Rodriguez IP, Chakravarthi BV and Varambally S: UALCAN: A portal for facilitating tumor subgroup gene expression and survival analyses. Neoplasia. 19:649–658. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Holland EC: Gliomagenesis: Genetic alterations and mouse models. Nat Rev Genet. 2:120–129. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Nakada M, Nakada S, Demuth T, Tran NL, Hoelzinger DB and Berens ME: Molecular targets of glioma invasion. Cell Mol Life Sci. 64:458–478. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Ersahin T, Tuncbag N and Cetin-Atalay R: The PI3K/AKT/mTOR interactive pathway. Mol Biosyst. 11:1946–1954. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Lamouille S, Xu J and Derynck R: Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 15:178–196. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Gonzalez DM and Medici D: Signaling mechanisms of the epithelial-mesenchymal transition. Sci Signal. 7:re82014. View Article : Google Scholar : PubMed/NCBI

32 

Popolo A, Pinto A, Daglia M, Nabavi SF, Farooqi AA and Rastrelli L: Two likely targets for the anti-cancer effect of indole derivatives from cruciferous vegetables: PI3K/Akt/mTOR signalling pathway and the aryl hydrocarbon receptor. Semin Cancer Biol. 46:132–137. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Lee HJ, Venkatarame Gowda Saralamma V, Kim SM, Ha SE, Raha S, Lee WS, Kim EH, Lee SJ, Heo JD and Kim GS: Pectolinarigenin induced cell cycle arrest, autophagy, and apoptosis in gastric cancer cell via PI3K/AKT/mTOR signaling pathway. Nutrients. 10:10432018. View Article : Google Scholar

34 

Zhang H, Xu HL, Wang YC, Lu ZY, Yu XF and Sui DY: 20(S)-protopanaxadiol-induced apoptosis in MCF-7 breast cancer cell line through the inhibition of PI3K/AKT/mTOR signaling pathway. Int J Mol Sci. 19:10532018. View Article : Google Scholar

35 

Larue L and Bellacosa A: Epithelial-mesenchymal transition in development and cancer: Role of phosphatidylinositol 3′ kinase/AKT pathways. Oncogene. 24:7443–7454. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Vanhaesebroeck B, Guillermet-Guibert J, Graupera M and Bilanges B: The emerging mechanisms of isoform-specific PI3K signalling. Nat Rev Mol Cell Biol. 11:329–341. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Geisbrecht BV and Gould SJ: The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J Biol Chem. 274:30527–30533. 1999. View Article : Google Scholar : PubMed/NCBI

38 

Dimitrov L, Hong CS, Yang C, Zhuang Z and Heiss JD: New developments in the pathogenesis and therapeutic targeting of the IDH1 mutation in glioma. Int J Med Sci. 12:201–213. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Xu X, Zhao J, Xu Z, Peng B, Huang Q, Arnold E and Ding J: Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. J Biol Chem. 279:33946–33957. 2004. View Article : Google Scholar : PubMed/NCBI

40 

Janku F, Yap TA and Meric-Bernstam F: Targeting the PI3K pathway in cancer: Are we making headway? Nat Rev Clin Oncol. 15:273–291. 2018. View Article : Google Scholar : PubMed/NCBI

41 

Birner P, Pusch S, Christov C, Mihaylova S, Uzeir KT, Natchev S, Schoppmann SF, Tchorbanov A, Streubel B, Tuettenberg J and Guentchev M: Mutant IDH1 inhibits PI3K/Akt signaling in human glioma. Cancer. 120:2440–2447. 2014. View Article : Google Scholar : PubMed/NCBI

42 

Noushmehr H, Weisenberger DJ, Diefes K, Phillips HS, Pujara K, Berman BP, Pan F, Pelloski CE, Sulman EP, Bhat KP, et al: Identification of a CpG island methylator phenotype that defines a distinct subgroup of glioma. Cancer Cell. 17:510–522. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Bralten LB, Kloosterhof NK, Balvers R, Sacchetti A, Lapre L, Lamfers M, Leenstra S, Jonge Hd, Kros JM, Jansen EEW, et al: IDH1 R132H decreases proliferation of glioma cell lines in vitro and in vivo. Ann Neurol. 69:455–463. 2011. View Article : Google Scholar : PubMed/NCBI

44 

Zhu H, Zhang Y, Chen J, Qiu J, Huang K, Wu M and Xia C: IDH1 R132H mutation enhances cell migration by activating AKT-mTOR signaling pathway, but sensitizes cells to 5-FU treatment as nadph and gsh are reduced. PLoS One. 12:e01690382017. View Article : Google Scholar : PubMed/NCBI

45 

Carbonneau M, Gagné LM, Lalonde ME, Germain MA, Motorina A, Guiot MC, Secco B, Vincent EE, Tumber A, Hulea L, et al: The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 7:127002016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shen X, Wu S, Zhang J, Li M, Xu F, Wang A, Lei Y and Zhu G: Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells. Mol Med Rep 22: 1949-1957, 2020.
APA
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A. ... Zhu, G. (2020). Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells. Molecular Medicine Reports, 22, 1949-1957. https://doi.org/10.3892/mmr.2020.11250
MLA
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A., Lei, Y., Zhu, G."Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells". Molecular Medicine Reports 22.3 (2020): 1949-1957.
Chicago
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A., Lei, Y., Zhu, G."Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells". Molecular Medicine Reports 22, no. 3 (2020): 1949-1957. https://doi.org/10.3892/mmr.2020.11250
Copy and paste a formatted citation
x
Spandidos Publications style
Shen X, Wu S, Zhang J, Li M, Xu F, Wang A, Lei Y and Zhu G: Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells. Mol Med Rep 22: 1949-1957, 2020.
APA
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A. ... Zhu, G. (2020). Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells. Molecular Medicine Reports, 22, 1949-1957. https://doi.org/10.3892/mmr.2020.11250
MLA
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A., Lei, Y., Zhu, G."Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells". Molecular Medicine Reports 22.3 (2020): 1949-1957.
Chicago
Shen, X., Wu, S., Zhang, J., Li, M., Xu, F., Wang, A., Lei, Y., Zhu, G."Wild‑type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells". Molecular Medicine Reports 22, no. 3 (2020): 1949-1957. https://doi.org/10.3892/mmr.2020.11250
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