Anti‑inflammatory activity of 3,5,6,7,3',4'‑hexamethoxyflavone via repression of the NF‑κB and MAPK signaling pathways in LPS‑stimulated RAW264.7 cells

Son,Eun Suk; Park,Jeong‑Woong; Kim,Se‑Hee; Park,Hye Ran; Han,Woorijarang; Kwon,O Chul; Nam,Jae Young; Jeong,Sung Hwan; Lee,Chang Soo

doi:10.3892/mmr.2020.11252

Anti‑inflammatory activity of 3,5,6,7,3',4'‑hexamethoxyflavone via repression of the NF‑κB and MAPK signaling pathways in LPS‑stimulated RAW264.7 cells

Authors:
- Eun Suk Son
- Jeong‑Woong Park
- Se‑Hee Kim
- Hye Ran Park
- Woorijarang Han
- O Chul Kwon
- Jae Young Nam
- Sung Hwan Jeong
- Chang Soo Lee
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Affiliations: Department of Biomedical Chemistry, Konkuk University, Chungju, Chungcheongbuk 27478, Republic of Korea, Department of Allergy, Pulmonary and Critical Care Medicine, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea, Gachon Medical Research Institute, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea, Company Affiliated Research Institute, Seongnam, Gyeonggi 13511, Republic of Korea
Published online on: June 18, 2020 https://doi.org/10.3892/mmr.2020.11252
Pages: 1985-1993
Copyright: © Son et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Citrus peel has been used as a Traditional medicine in Asia to treat coughs, asthma and bronchial disorders. Therefore, the anti‑inﬂammatory effects of 3,5,6,7,3',4'‑hexamethoxyflavone (quercetogetin, QUE) isolated from Citrus unshiu peel were investigated in lipopolysaccharide (LPS)‑induced RAW 264.7 macrophage cells. The results showed that QUE repressed the production of prostaglandin E2 and nitric oxide by suppressing LPS‑induced expression of cyclooxygenase‑2 and inducible nitric oxide synthase. It also suppressed the production of interleukin (IL)‑6, IL‑1β, and tumor necrosis factor‑α cytokines, and decreased the nuclear translocation of NF‑κB by interrupting the phosphorylation of NF‑κB inhibitor α in macrophage cells. Based on the finding that QUE inhibited the phosphorylation of ERK protein expression in LPS‑induced RAW264.7 cells, it was confirmed that inhibition of inflammatory responses by QUE was mediated via the ERK pathway. Therefore, this study suggests that QUE has strong anti‑inflammatory effects, making it a promising compound for use as a therapeutic agent in treating inflammatory lung diseases, such as emphysema.

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