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Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit

  • Authors:
    • Kwanhoon Choi
    • Bon‑Hyeock Koo
    • Byeong Jun Yoon
    • Minkyo Jung
    • Hye Young Yun
    • Byung Hwa Jeon
    • Moo‑Ho Won
    • Young‑Myeong Kim
    • Ji Young Mun
    • Hyun Kyo Lim
    • Sungwoo Ryoo
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Pain Medicine, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea, Department of Biological Sciences, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Neural Circuits Research, Korea Brain Research Institute, Dong, Daegu 41068, Republic of Korea, Department of Physiology, School of Medicine, Chungnam National University, Daejeon 34134, Republic of Korea, Department of Neurobiology, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Molecular and Cellular Biochemistry, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
    Copyright: © Choi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2395-2403
    |
    Published online on: July 6, 2020
       https://doi.org/10.3892/mmr.2020.11307
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Abstract

The p32 protein plays a crucial role in the regulation of cytosolic Ca2+ concentrations ([Ca2+]c) that contributes to the Ca2+‑dependent signaling cascade. Using an adenovirus and plasmid p32‑overexpression system, the aim of the study was to evaluate the role of p32 in the regulation of [Ca2+] and its potential associated with Ca2+‑dependent endothelial nitric oxide synthase (eNOS) activation in endothelial cells. Using electron and confocal microscopic analysis, p32 overexpression was observed to be localized to mitochondria and the endoplasmic reticulum and played an important role in Ca2+ translocation, resulting in increased [Ca2+] in these organelles and reducing cytosolic [Ca2+] ([Ca2+]c). This decreased [Ca2+]c following p32 overexpression attenuated the Ca2+‑dependent signaling cascade of calcium/calmodulin dependent protein kinase II (CaMKII)/AKT/eNOS phosphorylation. Moreover, in aortic endothelia of wild‑type mice intravenously administered adenovirus encoding the p32 gene, increased p32 levels reduced NO production and accelerated reactive oxygen species (ROS) generation. In a vascular tension assay, p32 overexpression decreased acetylcholine (Ach)‑induced vasorelaxation and augmented phenylephrine (PE)‑dependent vasoconstriction. Notably, decreased levels of arginase II (ArgII) protein using siArgII were associated with downregulation of overexpressed p32 protein, which contributed to CaMKII‑dependent eNOS phosphorylation at Ser1177. These results indicated that increased protein levels of p32 caused endothelial dysfunction through attenuation of the Ca2+‑dependent signaling cascade and that ArgII protein participated in the stability of p32. Therefore, p32 may be a novel target for the treatment of vascular diseases associated with endothelial disorders.
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1 

Ghebrehiwet B, Lim BL, Peerschke EI, Willis AC and Reid KB: Isolation, cDNA cloning, and overexpression of a 33-kD cell surface glycoprotein that binds to the globular ‘heads’ of C1q. J Exp Med. 179:1809–1821. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Krainer AR, Mayeda A, Kozak D and Binns G: Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell. 66:383–394. 1991. View Article : Google Scholar : PubMed/NCBI

3 

Muta T, Kang D, Kitajima S, Fujiwara T and Hamasaki N: p32 protein, a splicing factor 2-associated protein, is localized in mitochondrial matrix and is functionally important in maintaining oxidative phosphorylation. J Biol Chem. 272:24363–24370. 1997. View Article : Google Scholar : PubMed/NCBI

4 

Sengupta A, Banerjee B, Tyagi RK and Datta K: Golgi localization and dynamics of hyaluronan binding protein 1 (HABP1/p32/C1QBP) during the cell cycle. Cell Res. 15:183–186. 2005. View Article : Google Scholar : PubMed/NCBI

5 

van Leeuwen HC and O'Hare P: Retargeting of the mitochondrial protein p32/gC1Qr to a cytoplasmic compartment and the cell surface. J Cell Sci. 114:2115–2123. 2001.PubMed/NCBI

6 

Itahana K and Zhang Y: Mitochondrial p32 is a critical mediator of ARF-induced apoptosis. Cancer Cell. 13:542–553. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Sunayama J, Ando Y, Itoh N, Tomiyama A, Sakurada K, Sugiyama A, Kang D, Tashiro F, Gotoh Y, Kuchino Y, et al: Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32. Cell Death Differ. 11:771–781. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Fogal V, Richardson AD, Karmali PP, Scheffler IE, Smith JW and Ruoslahti E: Mitochondrial p32 protein is a critical regulator of tumor metabolism via maintenance of oxidative phosphorylation. Mol Cell Biol. 30:1303–1318. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Hu M, Crawford SA, Henstridge DC, Ng IH, Boey EJ, Xu Y, Febbraio MA, Jans DA and Bogoyevitch MA: p32 protein levels are integral to mitochondrial and endoplasmic reticulum morphology, cell metabolism and survival. Biochem J. 453:381–391. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Pupo AS and Minneman KP: Specific interactions between gC1qR and alpha1-adrenoceptor subtypes. J Recept Signal Transduct Res. 23:185–195. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Storz P, Hausser A, Link G, Dedio J, Ghebrehiwet B, Pfizenmaier K and Johannes FJ: Protein kinase C [micro] is regulated by the multifunctional chaperon protein p32. J Biol Chem. 275:24601–24607. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Simos G and Georgatos SD: The lamin B receptor-associated protein p34 shares sequence homology and antigenic determinants with the splicing factor 2-associated protein p32. FEBS Lett. 346:225–228. 1994. View Article : Google Scholar : PubMed/NCBI

13 

Deb TB and Datta K: Molecular cloning of human fibroblast hyaluronic acid-binding protein confirms its identity with P-32, a protein co-purified with splicing factor SF2. Hyaluronic acid-binding protein as P-32 protein, co-purified with splicing factor SF2. J Biol Chem. 271:2206–2212. 1996. View Article : Google Scholar : PubMed/NCBI

14 

Lim BL, Reid KB, Ghebrehiwet B, Peerschke EI, Leigh LA and Preissner KT: The binding protein for globular heads of complement C1q, gC1qR. Functional expression and characterization as a novel vitronectin binding factor. J Biol Chem. 271:26739–26744. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Yu L, Zhang Z, Loewenstein PM, Desai K, Tang Q, Mao D, Symington JS and Green M: Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domain. J Virol. 69:3007–3016. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Wang Y, Finan JE, Middeldorp JM and Hayward SD: P32/TAP, a cellular protein that interacts with EBNA-1 of Epstein-Barr virus. Virology. 236:18–29. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Braun L, Ghebrehiwet B and Cossart P: gC1q-R/p32, a C1q-binding protein, is a receptor for the InlB invasion protein of Listeria monocytogenes. EMBO J. 19:1458–1466. 2000. View Article : Google Scholar : PubMed/NCBI

18 

Fleming I, Fisslthaler B, Dimmeler S, Kemp BE and Busse R: Phosphorylation of Thr(495) regulates Ca(2+)/calmodulin-dependent endothelial nitric oxide synthase activity. Circ Res. 88:E68–E75. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Salerno JC, Harris DE, Irizarry K, Patel B, Morales AJ, Smith SM, Martasek P, Roman LJ, Masters BS, Jones CL, et al: An autoinhibitory control element defines calcium-regulated isoforms of nitric oxide synthase. J Biol Chem. 272:29769–29777. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Lobatón CD, Vay L, Hernández-Sanmiguel E, Santodomingo J, Moreno A, Montero M and Alvarez J: Modulation of mitochondrial Ca(2+) uptake by estrogen receptor agonists and antagonists. Br J Pharmacol. 145:862–871. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Koo BH, Hwang HM, Yi BG, Lim HK, Jeon BH, Hoe KL, Kwon YG, Won MH, Kim YM, Berkowitz DE, et al: Arginase II Contributes to the Ca2+/CaMKII/eNOS Axis by Regulating Ca2+ Concentration Between the Cytosol and Mitochondria in a p32-Dependent Manner. J Am Heart Assoc. 7:e0095792018. View Article : Google Scholar : PubMed/NCBI

22 

Ryoo S, Gupta G, Benjo A, Lim HK, Camara A, Sikka G, Lim HK, Sohi J, Santhanam L, Soucy K, et al: Endothelial arginase II: A novel target for the treatment of atherosclerosis. Circ Res. 102:923–932. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Peerschke EI and Ghebrehiwet B: The contribution of gC1qR/p33 in infection and inflammation. Immunobiology. 212:333–342. 2007. View Article : Google Scholar : PubMed/NCBI

24 

24. Koo BH, Hong D, Hong HD, Lim HK, Hoe KL, Won M-H, Kim YM, Berkowitz DE and Ryoo S: Arginase II activity regulates cytosolic Ca(2+) level in a p32-dependent manner that contributes to Ca(2+)-dependent vasoconstriction in native low-density lipoprotein-stimulated vascular smooth muscle cells. Exp Mol Med. 51:1–12. 2019. View Article : Google Scholar

25 

Sessa WC: eNOS at a glance. J Cell Sci. 117:2427–2429. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Ignarro LJ, Buga GM, Wei LH, Bauer PM, Wu G and del Soldato P: Role of the arginine-nitric oxide pathway in the regulation of vascular smooth muscle cell proliferation. Proc Natl Acad Sci USA. 98:4202–4208. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Li H, Meininger CJ, Hawker JR Jr, Haynes TE, Kepka-Lenhart D, Mistry SK, Morris SM Jr and Wu G: Regulatory role of arginase I and II in nitric oxide, polyamine, and proline syntheses in endothelial cells. Am J Physiol Endocrinol Metab. 280:E75–E82. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Morris SM Jr, Kepka-Lenhart D and Chen LC: Differential regulation of arginases and inducible nitric oxide synthase in murine macrophage cells. Am J Physiol. 275:E740–E747. 1998.PubMed/NCBI

29 

Louis CA, Reichner JS, Henry WL Jr, Mastrofrancesco B, Gotoh T, Mori M and Albina JE: Distinct arginase isoforms expressed in primary and transformed macrophages: Regulation by oxygen tension. Am J Physiol. 274:R775–R782. 1998.PubMed/NCBI

30 

Collado B, Sánchez-Chapado M, Prieto JC and Carmena MJ: Hypoxia regulation of expression and angiogenic effects of vasoactive intestinal peptide (VIP) and VIP receptors in LNCaP prostate cancer cells. Mol Cell Endocrinol. 249:116–122. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Berkowitz DE, White R, Li D, Minhas KM, Cernetich A, Kim S, Burke S, Shoukas AA, Nyhan D, Champion HC, et al: Arginase reciprocally regulates nitric oxide synthase activity and contributes to endothelial dysfunction in aging blood vessels. Circulation. 108:2000–2006. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Hein TW, Zhang C, Wang W, Chang CI, Thengchaisri N and Kuo L: Ischemia-reperfusion selectively impairs nitric oxide-mediated dilation in coronary arterioles: Counteracting role of arginase. FASEB J. 17:2328–2330. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Jung C, Gonon AT, Sjöquist PO, Lundberg JO and Pernow J: Arginase inhibition mediates cardioprotection during ischaemia-reperfusion. Cardiovasc Res. 85:147–154. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Zhang C, Hein TW, Wang W, Miller MW, Fossum TW, McDonald MM, Humphrey JD and Kuo L: Upregulation of vascular arginase in hypertension decreases nitric oxide-mediated dilation of coronary arterioles. Hypertension. 44:935–943. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Johnson FK, Johnson RA, Peyton KJ and Durante W: Arginase inhibition restores arteriolar endothelial function in Dahl rats with salt-induced hypertension. Am J Physiol Regul Integr Comp Physiol. 288:R1057–R1062. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Peyton KJ, Ensenat D, Azam MA, Keswani AN, Kannan S, Liu XM, Wang H, Tulis DA and Durante W: Arginase promotes neointima formation in rat injured carotid arteries. Arterioscler Thromb Vasc Biol. 29:488–494. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Choi K, Koo BH, Yoon BJ, Jung M, Yun HY, Jeon BH, Won MH, Kim YM, Mun JY, Lim HK, Lim HK, et al: Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit. Mol Med Rep 22: 2395-2403, 2020.
APA
Choi, K., Koo, B., Yoon, B.J., Jung, M., Yun, H.Y., Jeon, B.H. ... Ryoo, S. (2020). Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit. Molecular Medicine Reports, 22, 2395-2403. https://doi.org/10.3892/mmr.2020.11307
MLA
Choi, K., Koo, B., Yoon, B. J., Jung, M., Yun, H. Y., Jeon, B. H., Won, M., Kim, Y., Mun, J. Y., Lim, H. K., Ryoo, S."Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit". Molecular Medicine Reports 22.3 (2020): 2395-2403.
Chicago
Choi, K., Koo, B., Yoon, B. J., Jung, M., Yun, H. Y., Jeon, B. H., Won, M., Kim, Y., Mun, J. Y., Lim, H. K., Ryoo, S."Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit". Molecular Medicine Reports 22, no. 3 (2020): 2395-2403. https://doi.org/10.3892/mmr.2020.11307
Copy and paste a formatted citation
x
Spandidos Publications style
Choi K, Koo BH, Yoon BJ, Jung M, Yun HY, Jeon BH, Won MH, Kim YM, Mun JY, Lim HK, Lim HK, et al: Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit. Mol Med Rep 22: 2395-2403, 2020.
APA
Choi, K., Koo, B., Yoon, B.J., Jung, M., Yun, H.Y., Jeon, B.H. ... Ryoo, S. (2020). Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit. Molecular Medicine Reports, 22, 2395-2403. https://doi.org/10.3892/mmr.2020.11307
MLA
Choi, K., Koo, B., Yoon, B. J., Jung, M., Yun, H. Y., Jeon, B. H., Won, M., Kim, Y., Mun, J. Y., Lim, H. K., Ryoo, S."Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit". Molecular Medicine Reports 22.3 (2020): 2395-2403.
Chicago
Choi, K., Koo, B., Yoon, B. J., Jung, M., Yun, H. Y., Jeon, B. H., Won, M., Kim, Y., Mun, J. Y., Lim, H. K., Ryoo, S."Overexpressed p32 localized in the endoplasmic reticulum and mitochondria negatively regulates calcium‑dependent endothelial nitric oxide synthase activit". Molecular Medicine Reports 22, no. 3 (2020): 2395-2403. https://doi.org/10.3892/mmr.2020.11307
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