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Article

ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells

  • Authors:
    • Na Wang
    • Hua Wang
    • Jianming Chen
    • Fubin Wang
    • Shuaiyi Wang
    • Qiang Zhou
    • Jichong Ying
    • Shanzhao Huang
    • Pu Wang
    • Fangfang Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Radiology, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Orthopaedics, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Inspection, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Endocrinology, The First Hospital of Jiaxing (The Affiliated Hospital of Jiaxing University), Jiaxing, Zhejiang 314001, P.R. China, Department of Osteopeniology, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Cardiology, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China, Department of Rheumatology, Ningbo Number 6 Hospital, Ningbo, Zhejiang 315040, P.R. China
  • Pages: 2451-2459
    |
    Published online on: July 9, 2020
       https://doi.org/10.3892/mmr.2020.11319
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Abstract

Glucocorticoid‑induced osteoporosis is the commonest form of drug‑induced osteoporosis. Histone deacetylase 6 (HDAC6) is involved in the differentiation from mesenchymal stem cells to osteoblasts. However, the role of ricolinostat (ACY‑1215, HDAC6 inhibitor) in the dexamethasone (Dex)‑induced proliferation and differentiation of preosteoblasts remains to be elucidated. The protein expression and mRNA expression levels of HDAC6, osteopontin (OPN), runt‑related transcription factor 2 (Runx2), osterix (Osx), collagen I (COL1A1) and glucocorticoid receptor (GR) in MC3T3‑E1 cells were analyzed by western blot analysis and reverse transcription‑quantitative PCR analysis. The cell viability was detected by CCK‑8 assay. The alkaline phosphatase (ALP) activity and capacity of mineralization was determined by ALP assay kit and alizarin red staining. HDAC6 expression was increased in patient serum and Dex‑induced MC3T3‑E1 cells at a certain concentration range; 1 µM Dex was selected for further experimentation. Cell viability was decreased after Dex induction and restored following ACY‑1215 treatment. The ALP activity and capability for mineralization was decreased when MC3T3‑E1 cells were induced by 1 µM Dex and was gradually improved by the treatment of ACY‑1215 at 1, 5 and 10 mM. The expression of OPN, Runx2, Osx and COL1A1 was similar, with the changes of capability for mineralization. Furthermore, GR expression was increased in Dex‑induced MC3T3‑E1 cells. ACY‑1215 promoted the GR expression in MC3T3‑E1 cells from 1‑5 mM while GR receptor expression was increased with 10 mM ACY‑1215 treatment. In conclusion, ACY‑1215 reversed the Dex‑induced suppression of proliferation and differentiation of MC3T3‑E1 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang N, Wang H, Chen J, Wang F, Wang S, Zhou Q, Ying J, Huang S, Wang P, Yuan F, Yuan F, et al: ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells. Mol Med Rep 22: 2451-2459, 2020.
APA
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q. ... Yuan, F. (2020). ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells. Molecular Medicine Reports, 22, 2451-2459. https://doi.org/10.3892/mmr.2020.11319
MLA
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q., Ying, J., Huang, S., Wang, P., Yuan, F."ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells". Molecular Medicine Reports 22.3 (2020): 2451-2459.
Chicago
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q., Ying, J., Huang, S., Wang, P., Yuan, F."ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells". Molecular Medicine Reports 22, no. 3 (2020): 2451-2459. https://doi.org/10.3892/mmr.2020.11319
Copy and paste a formatted citation
x
Spandidos Publications style
Wang N, Wang H, Chen J, Wang F, Wang S, Zhou Q, Ying J, Huang S, Wang P, Yuan F, Yuan F, et al: ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells. Mol Med Rep 22: 2451-2459, 2020.
APA
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q. ... Yuan, F. (2020). ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells. Molecular Medicine Reports, 22, 2451-2459. https://doi.org/10.3892/mmr.2020.11319
MLA
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q., Ying, J., Huang, S., Wang, P., Yuan, F."ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells". Molecular Medicine Reports 22.3 (2020): 2451-2459.
Chicago
Wang, N., Wang, H., Chen, J., Wang, F., Wang, S., Zhou, Q., Ying, J., Huang, S., Wang, P., Yuan, F."ACY‑1215, a HDAC6 inhibitor, decreases the dexamethasone‑induced suppression of osteogenesis in MC3T3‑E1 cells". Molecular Medicine Reports 22, no. 3 (2020): 2451-2459. https://doi.org/10.3892/mmr.2020.11319
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