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Article Open Access

Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro

  • Authors:
    • Le Zhuang
    • Weiyuan Ma
    • Jianjun Yan
    • Hua Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2715-2722
    |
    Published online on: July 20, 2020
       https://doi.org/10.3892/mmr.2020.11348
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Abstract

Psoriasis is one of the most common chronic inflammatory skin diseases, it is characterized by hyperproliferation of keratinocytes and infiltration of inflammatory cells. Several in vitro studies have reported that interleukin (IL)‑22 is involved in excessive proliferation and abnormal differentiation of human keratinocytes. However, the association between IL‑22 and CCAAT enhancer binding protein α (C/EBPα) in the pathogenesis of psoriasis remains unclear. Therefore, the present study aimed to investigate the association between IL‑22 and C/EBPα, and the effects of IL‑22 on the proliferation and differentiation of keratinocytes. Keratinocytes were treated with different concentrations of IL‑22 (30, 60 and 90 ng/ml) and subsequently cells were collected at different time intervals. The expression levels of the key molecules of the mitogen‑activated protein kinase (MAPK) signaling pathway were detected using western blot analysis. In addition, the effect of IL‑22 on the proliferation rate of keratinocytes and the mRNA expression levels of C/EBPα were determined using a Cell Counting Kit‑8 assay and reverse transcription‑quantitative PCR, respectively. Furthermore, keratinocytes were transfected with C/EBPα small interfering (si)RNA or control using Lipofectamine® 2000. The results revealed that IL‑22 significantly induced the proliferation of keratinocytes and the expression of phosphorylated (p)‑JNK, p‑ERK and p‑p38 (P<0.05). Additionally, IL‑22 significantly inhibited the differentiation of keratinocytes, and the mRNA and protein expression of C/EBPα (P<0.05). Furthermore, downregulation of C/EBPα increased the proliferation rate of keratinocytes and reduced the expression levels of cytokeratin 10 and involucrin. Therefore, these results suggested that the effect of IL‑22 on the proliferation and differentiation of keratinocytes may be mediated via the regulation of the MAPK signaling pathway and the expression of C/EBPα.
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Copy and paste a formatted citation
Spandidos Publications style
Zhuang L, Ma W, Yan J and Zhong H: Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro. Mol Med Rep 22: 2715-2722, 2020.
APA
Zhuang, L., Ma, W., Yan, J., & Zhong, H. (2020). Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro. Molecular Medicine Reports, 22, 2715-2722. https://doi.org/10.3892/mmr.2020.11348
MLA
Zhuang, L., Ma, W., Yan, J., Zhong, H."Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro". Molecular Medicine Reports 22.4 (2020): 2715-2722.
Chicago
Zhuang, L., Ma, W., Yan, J., Zhong, H."Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro". Molecular Medicine Reports 22, no. 4 (2020): 2715-2722. https://doi.org/10.3892/mmr.2020.11348
Copy and paste a formatted citation
x
Spandidos Publications style
Zhuang L, Ma W, Yan J and Zhong H: Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro. Mol Med Rep 22: 2715-2722, 2020.
APA
Zhuang, L., Ma, W., Yan, J., & Zhong, H. (2020). Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro. Molecular Medicine Reports, 22, 2715-2722. https://doi.org/10.3892/mmr.2020.11348
MLA
Zhuang, L., Ma, W., Yan, J., Zhong, H."Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro". Molecular Medicine Reports 22.4 (2020): 2715-2722.
Chicago
Zhuang, L., Ma, W., Yan, J., Zhong, H."Evaluation of the effects of IL‑22 on the proliferation and differentiation of keratinocytes in vitro". Molecular Medicine Reports 22, no. 4 (2020): 2715-2722. https://doi.org/10.3892/mmr.2020.11348
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