Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
October-2020 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2020 Volume 22 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells

Retraction in: /10.3892/mmr.2025.13519
  • Authors:
    • Chenglong Li
    • Lu Yang
    • Xiaohua Ren
    • Mu Lin
    • Daonan Shen
    • You Li
    • Xiangyu Zhang
    • Chunhui Liu
    • Yandong Mu
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, P.R. China, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, P.R. China, West China School and Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610063, P.R. China, Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2801-2809
    |
    Published online on: July 23, 2020
       https://doi.org/10.3892/mmr.2020.11352
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hydroxyapatite scaffolds (HASs) are widely studied as suitable materials for bone replacement scaffolds due to their chemical similarities to organic materials. In our previous study, a novel HAS with a 25‑30‑µm groove structure (HAS‑G) exhibited enhanced osteogenesis of bone mesenchymal stromal cells (BMSCs) compared with HAS, potentially by modulating the macrophage‑induced immune microenvironment. However, the exact effects of different surface patterns on the physiological processes of attached cells is not known. The present study aimed to determine the effects of HAS‑G on the osteogenesis and physiological processes in BMSCs. Cell counting kit‑8 assays and propidium iodide staining followed by flow cytometry were performed, and the results demonstrated that both in normal medium and differentiating medium, HAS‑G promoted cell proliferation by decreasing the proportion of G1/G0 cells and decreased reactive oxygen species (ROS) accumulation in BMSCs compared with HAS. Detection markers of osteogenesis revealed that compared with HAS, HAS‑G increased runt‑related transcription factor 2, osteocalcin and osteopontin protein levels and promoted osteogenesis, which was further confirmed by Alizarin Red S staining. Following JC‑1 staining, it was observed that HAS‑G maintained the mitochondrial membrane potential, similar to that achieved by N‑acetylcysteine pretreatment. In addition, compared with those of HAS, HAS‑G decreased mitochondrial ROS levels, which potentially contributed to the promotion of osteogenesis. The results also demonstrated that HAS‑G inhibited mitophagy induced by ROS accumulation and ATP synthesis compared with HAS. In conclusion, HAS‑G decreased ROS accumulation and mitophagy and thus promoted osteogenesis of BMSCs, indicating that ROS modulation of HAS‑G may serve a key role in osteogenesis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jiang J, Hao W, Li Y, Yao J, Shao Z, Li H, Yang J and Chen S: Hydroxyapatite/regenerated silk fibroin scaffold-enhanced osteoinductivity and osteoconductivity of bone marrow-derived mesenchymal stromal cells. Biotechnol Lett. 35:2801–661. 2013. View Article : Google Scholar

2 

Wang H, Zhi W, Lu X, Li X, Duan K, Duan R, Mu Y and Weng J: Comparative studies on ectopic bone formation in porous hydroxyapatite scaffolds with complementary pore structures. Acta Biomater. 9:8413–8421. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Pallela R, Venkatesan J, Janapala VR and Kim SK: Biophysicochemical evaluation of chitosan-hydroxyapatite-marine sponge collagen composite for bone tissue engineering. J Biomed Mater Res A. 100:486–495. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Hutmacher DW: Scaffolds in tissue engineering bone and cartilage. Biomaterials. 21:2529–2543. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Zhou Y, Yao H, Wang J, Wang D, Liu Q and Li Z: Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering. Int J Nanomedicine. 10:3203–3215. 2015.PubMed/NCBI

6 

D'Agostino A, Trevisiol L, Favero V, Gunson MJ, Pedica F, Nocini PF and Arnett GW: Hydroxyapatite/Collagen composite is a reliable material for malar augmentation. J Oral Maxillofac Surg. 74:1238.e1–1238.e15. 2016. View Article : Google Scholar

7 

Zhang Q, Dong H, Li Y, Zhu Y, Zeng L, Gao H, Yuan B, Chen X and Mao C: Microgrooved polymer substrates promote collective cell migration to accelerate fracture healing in an in vitro model. ACS Appl Mater Interfaces. 7:23336–23345. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Ren X, Tuo Q, Tian K, Huang G, Li J, Xu T, Lv X, Wu J, Chen Z, Weng J, et al: Enhancement of osteogenesis using a novel porous hydroxyapatite scaffold in vivo and vitro. Ceram Int. 44:21656–21665. 2018. View Article : Google Scholar

9 

Li C, Yang L, Ren X, Lin M, Jiang X, Shen D, Xu T, Ren J, Huang L, Qing W, et al: Groove structure of porous hydroxyapatite scaffolds (HAS) modulates immune environment via regulating macrophages and subsequently enhances osteogenesis. J Biol Inorg Chem. 24:733–745. 2019. View Article : Google Scholar : PubMed/NCBI

10 

Bianco P, Sacchetti B and Riminucci M: Stem cells in skeletal physiology and endocrine diseases of bone. Endocr Dev. 21:91–101. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Gong J, Meng HB, Hua J, Song ZS, He ZG, Zhou B and Qian MP: The SDF-1/CXCR4 axis regulates migration of transplanted bone marrow mesenchymal stem cells towards the pancreas in rats with acute pancreatitis. Mol Med Rep. 9:1575–1582. 2014. View Article : Google Scholar : PubMed/NCBI

12 

van Balkom BW, de Jong OG, Smits M, Brummelman J, den Ouden K, de Bree PM, van Eijndhoven MA, Pegtel DM, Stoorvogel W, Würdinger T and Verhaar MC: Endothelial cells require miR-214 to secrete exosomes that suppress senescence and induce angiogenesis in human and mouse endothelial cells. Blood. 121:3997–4006, S1-S15. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Ding R, Feng L, He L, Chen Y, Wen P, Fu Z, Lin C, Yang S, Deng X, Zeng J and Sun G: Peroxynitrite decomposition catalyst prevents matrix metalloproteinase-9 activation and neurovascular injury after hemoglobin injection into the caudate nucleus of rats. Neuroscience. 297:182–193. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Karlsson M and Tang L: Surface morphology and adsorbed proteins affect phagocyte responses to Nano-porous alumina. J Mater Sci Mater Med. 17:1101–1111. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Tsaryk R, Kalbacova M, Hempel U, Scharnweber D, Unger RE, Dieter P, Kirkpatrick CJ and Peters K: Response of human endothelial cells to oxidative stress on Ti6Al4V alloy. Biomaterials. 28:806–813. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Serrano MC, Pagani R, Peña J and Portolès MT: Transitory oxidative stress in L929 fibroblasts cultured on poly(epsilon-caprolactone) films. Biomaterials. 26:5827–5834. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Kaiser JP, Reinmann A and Bruinink A: The effect of topographic characteristics on cell migration velocity. Biomaterials. 27:5230–5241. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Walboomers XF, Monaghan W, Curtis AS and Jansen JA: Attachment of fibroblasts on smooth and microgrooved polystyrene. J Biomed Mater Res. 46:212–220. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Yang Y, Zhang Y, Chai R and Gu Z: Designs of biomaterials and microenvironments for neuroengineering. Neural Plast. 2018:10219692018. View Article : Google Scholar : PubMed/NCBI

20 

Roach PL, Clifton IJ, Hensgens CM, Shibata N, Schofield CJ, Hajdu J and Baldwin JE: Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation. Nature. 387:827–830. 1997. View Article : Google Scholar : PubMed/NCBI

21 

Rajnicek AM, Foubister LE and McCaig CD: Alignment of corneal and lens epithelial cells by co-operative effects of substratum topography and DC electric fields. Biomaterials. 29:2082–2095. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Feng ZQ, Lu HJ, Leach MK, Huang NP, Wang YC, Liu CJ and Gu ZZ: The influence of type-I collagen-coated PLLA aligned nanofibers on growth of blood outgrowth endothelial cells. Biomed Mater. 5:0650112010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li C, Yang L, Ren X, Lin M, Shen D, Li Y, Zhang X, Liu C and Mu Y: Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519. Mol Med Rep 22: 2801-2809, 2020.
APA
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y. ... Mu, Y. (2020). Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519. Molecular Medicine Reports, 22, 2801-2809. https://doi.org/10.3892/mmr.2020.11352
MLA
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y., Zhang, X., Liu, C., Mu, Y."Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519". Molecular Medicine Reports 22.4 (2020): 2801-2809.
Chicago
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y., Zhang, X., Liu, C., Mu, Y."Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519". Molecular Medicine Reports 22, no. 4 (2020): 2801-2809. https://doi.org/10.3892/mmr.2020.11352
Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Yang L, Ren X, Lin M, Shen D, Li Y, Zhang X, Liu C and Mu Y: Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519. Mol Med Rep 22: 2801-2809, 2020.
APA
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y. ... Mu, Y. (2020). Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519. Molecular Medicine Reports, 22, 2801-2809. https://doi.org/10.3892/mmr.2020.11352
MLA
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y., Zhang, X., Liu, C., Mu, Y."Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519". Molecular Medicine Reports 22.4 (2020): 2801-2809.
Chicago
Li, C., Yang, L., Ren, X., Lin, M., Shen, D., Li, Y., Zhang, X., Liu, C., Mu, Y."Grooved hydroxyapatite scaffold modulates mitochondria homeostasis and thus promotes osteogenesis in bone mesenchymal stromal cells Retraction in /10.3892/mmr.2025.13519". Molecular Medicine Reports 22, no. 4 (2020): 2801-2809. https://doi.org/10.3892/mmr.2020.11352
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team