Open Access

Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes

  • Authors:
    • Yanting Fan
    • Siyu Zhu
    • Jing Wang
    • Yuping Zhao
    • Xudong Wang
  • View Affiliations

  • Published online on: July 28, 2020     https://doi.org/10.3892/mmr.2020.11357
  • Pages: 2896-2904
  • Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Stroke is one of the leading causes of mortality and disability worldwide with limited clinical therapies available. The present study isolated primary astrocytes from the brains of rats and treated them with oxygen‑glucose deprivation and re‑oxygenation (OGD/R) to mimic hypoxia/reperfusion (H/R) injury in vitro to investigate stroke. It was revealed that propofol (2,6‑diisopropylphenol), an intravenous sedative and anesthetic agent, protected against oxygen/glucose‑deprivation (OGD) and induced cell injury. Furthermore, propofol exerted a protective effect by inhibiting gap junction function, which was also revealed to promote cell death in astrocytes. The present study further identified that propofol suppressed gap junction function by downregulating the protein expression levels of connexin43 (Cx43), which is one of the most essential components of gap junctions in astrocytes. In addition, when the expression levels of Cx43 were downregulated using small interfering RNA, OGD/R‑induced cell death was decreased. Conversely, cell death was enhanced when Cx43 was overexpressed, which was reversed following propofol treatment. In summary, propofol protects against OGD‑induced injury in astrocytes by decreasing the protein expression levels of Cx43 and suppressing gap junction function. The present study improved our understanding of how propofol protects astrocytes from OGD/R‑induced injury.
View Figures
View References

Related Articles

Journal Cover

October-2020
Volume 22 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Fan Y, Zhu S, Wang J, Zhao Y and Wang X: Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Mol Med Rep 22: 2896-2904, 2020
APA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., & Wang, X. (2020). Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Molecular Medicine Reports, 22, 2896-2904. https://doi.org/10.3892/mmr.2020.11357
MLA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22.4 (2020): 2896-2904.
Chicago
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22, no. 4 (2020): 2896-2904. https://doi.org/10.3892/mmr.2020.11357