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Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes

  • Authors:
    • Yanting Fan
    • Siyu Zhu
    • Jing Wang
    • Yuping Zhao
    • Xudong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong 510623, P.R. China, Department of Medical Imaging, Collaborative Innovation Center for Cancer Medicine, State Key Laboratory of Oncology in South China, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China, Department of Anesthesiology, State Key Laboratory of Oncology in South China, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China
    Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2896-2904
    |
    Published online on: July 28, 2020
       https://doi.org/10.3892/mmr.2020.11357
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Abstract

Stroke is one of the leading causes of mortality and disability worldwide with limited clinical therapies available. The present study isolated primary astrocytes from the brains of rats and treated them with oxygen‑glucose deprivation and re‑oxygenation (OGD/R) to mimic hypoxia/reperfusion (H/R) injury in vitro to investigate stroke. It was revealed that propofol (2,6‑diisopropylphenol), an intravenous sedative and anesthetic agent, protected against oxygen/glucose‑deprivation (OGD) and induced cell injury. Furthermore, propofol exerted a protective effect by inhibiting gap junction function, which was also revealed to promote cell death in astrocytes. The present study further identified that propofol suppressed gap junction function by downregulating the protein expression levels of connexin43 (Cx43), which is one of the most essential components of gap junctions in astrocytes. In addition, when the expression levels of Cx43 were downregulated using small interfering RNA, OGD/R‑induced cell death was decreased. Conversely, cell death was enhanced when Cx43 was overexpressed, which was reversed following propofol treatment. In summary, propofol protects against OGD‑induced injury in astrocytes by decreasing the protein expression levels of Cx43 and suppressing gap junction function. The present study improved our understanding of how propofol protects astrocytes from OGD/R‑induced injury.
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Copy and paste a formatted citation
Spandidos Publications style
Fan Y, Zhu S, Wang J, Zhao Y and Wang X: Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Mol Med Rep 22: 2896-2904, 2020.
APA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., & Wang, X. (2020). Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Molecular Medicine Reports, 22, 2896-2904. https://doi.org/10.3892/mmr.2020.11357
MLA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22.4 (2020): 2896-2904.
Chicago
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22, no. 4 (2020): 2896-2904. https://doi.org/10.3892/mmr.2020.11357
Copy and paste a formatted citation
x
Spandidos Publications style
Fan Y, Zhu S, Wang J, Zhao Y and Wang X: Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Mol Med Rep 22: 2896-2904, 2020.
APA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., & Wang, X. (2020). Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes. Molecular Medicine Reports, 22, 2896-2904. https://doi.org/10.3892/mmr.2020.11357
MLA
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22.4 (2020): 2896-2904.
Chicago
Fan, Y., Zhu, S., Wang, J., Zhao, Y., Wang, X."Propofol protects against oxygen/glucose deprivation‑induced cell injury via gap junction inhibition in astrocytes". Molecular Medicine Reports 22, no. 4 (2020): 2896-2904. https://doi.org/10.3892/mmr.2020.11357
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