Open Access

Assessment of plasma microRNAs in congenital intestinal malrotation

  • Authors:
    • Xiurui Lv
    • Huan Chen
    • Xinhe Sun
    • Lingling Zhou
    • Changgui Lu
    • Hongxing Li
  • View Affiliations

  • Published online on: July 31, 2020     https://doi.org/10.3892/mmr.2020.11395
  • Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intestinal malrotation in newborns often requires urgent surgical treatment, especially in the presence of volvulus. Therefore, early‑stage diagnosis is critical. In the present study, differentially expressed plasma microRNAs (miRNAs) were screened for in patients with intestinal malrotation using high‑throughput Illumina sequencing, and validated using reverse transcription‑quantitative PCR. Receiver operating characteristic curve (ROC) analysis was conducted to evaluate their specificity, sensitivity and assess their diagnostic value for intestinal malrotation. Bioinformatics analysis was performed to investigate the functions associated with the dysregulated miRNAs. A profile consisting of 28 differentially expressed plasma miRNAs was obtained, of which nine were verified to exhibit significantly altered expression. According to a ROC analysis, four of these could represent novel early‑stage, non‑invasive biomarkers for intestinal malrotation. Bioinformatics analysis demonstrated that the differentially expressed miRNAs were predominantly involved in ‘metal ion transmembrane transporter activity’ and ‘calcium‑dependent protein binding’, which may be related to the ‘endocytosis’ pathway. In conclusion, significantly differentially expressed plasma miRNAs were identified in congenital intestinal malrotation and their potential roles were described. These differentially expressed miRNAs may serve as biomarkers of intestinal malrotation and improve early diagnosis for this condition.

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Print ISSN: 1791-2997
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Copy and paste a formatted citation
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Spandidos Publications style
Lv X, Chen H, Sun X, Zhou L, Lu C and Li H: Assessment of plasma microRNAs in congenital intestinal malrotation. Mol Med Rep 0: 0-0, 1899
APA
Lv, X., Chen, H., Sun, X., Zhou, L., Lu, C., & Li, H. (1899). Assessment of plasma microRNAs in congenital intestinal malrotation. Molecular Medicine Reports, 0, 0-0. https://doi.org/10.3892/mmr.2020.11395
MLA
Lv, X., Chen, H., Sun, X., Zhou, L., Lu, C., Li, H."Assessment of plasma microRNAs in congenital intestinal malrotation". Molecular Medicine Reports 0.0 (1899): 0-0.
Chicago
Lv, X., Chen, H., Sun, X., Zhou, L., Lu, C., Li, H."Assessment of plasma microRNAs in congenital intestinal malrotation". Molecular Medicine Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/mmr.2020.11395