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Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats

  • Authors:
    • Xin Wang
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    Affiliations: Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110022, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3127-3134
    |
    Published online on: August 4, 2020
       https://doi.org/10.3892/mmr.2020.11410
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Abstract

Pneumonia is one of the commonest causes of death worldwide. High‑temperature requirement A2 (HtrA2) is a proapoptotic mitochondrial serine protease involved in caspase‑dependent or caspase‑independent cell apoptosis. UCF‑101 (5‑[5‑(2‑nitrophenyl) furfuryl iodine]‑1,3‑diphenyl‑2‑thiobarbituric acid), an inhibitor of HtrA2, has a protective effect on organs in various diseases by inhibiting cell apoptosis. The aim of the present study was to explore whether UCF‑101 has a protective effect on lungs in pneumonia. A lipopolysaccharide (LPS)‑induced pneumonia model was established in rats. UCF‑101 (2 µmol/kg) was used for treatment. Lung injury was detected by hematoxylin and eosin staining. Pro‑inflammatory cytokines and oxidative stress‑related factors were detected using corresponding test kits. TUNEL staining was used to measure the amount of cell apoptosis. Apoptosis‑associated proteins were detected by western blot assay. The present study indicated pulmonary injury induced by LPS. Treatment with UCF‑101 clearly alleviated this pulmonary damage and restored the levels of pro‑inflammatory cytokines and oxidative stress‑related factors. In addition, UCF‑101 significantly reduced LPS‑induced cell apoptosis, the release of HtrA2 and cytochrome from mitochondria to the cytoplasm and inhibited the expression of pro‑apoptotic proteins. UCF‑101 also restored the ATP level. The present results demonstrated that UCF‑101 acts as a positive regulator of acute pneumonia by inhibiting inflammatory response, oxidative stress and mitochondrial apoptosis. The present study suggests UCF‑101 as a potential candidate for pneumonia therapy.
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1 

Lutfiyya MN, Henley E, Chang LF and Reyburn SW: Diagnosis and treatment of community-acquired pneumonia. Am Fam Physician. 73:3127–450. 2006.

2 

Agweyu A, Kibore M, Digolo L, Kosgei C, Maina V, Mugane S, Muma S, Wachira J, Waiyego M and Maleche-Obimbo E: Prevalence and correlates of treatment failure among Kenyan children hospitalised with severe community-acquired pneumonia: A prospective study of the clinical effectiveness of WHO pneumonia case management guidelines. Trop Med Int Health. 19:1310–1320. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Bhutta ZA, Das JK, Walker N, Rizvi A, Campbell H, Rudan I and Black RE; Lancet Diarrhoea and Pneumonia Interventions Study Group, : Interventions to address deaths from childhood pneumonia and diarrhoea equitably: What works and at what cost? Lancet. 381:1417–1429. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Clausen T, Southan C and Ehrmann M: The HtrA family of proteases: Implications for protein composition and cell fate. Mol Cell. 10:443–455. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Bhuiyan MS and Fukunaga K: Mitochondrial serine protease HtrA2/Omi as a potential therapeutic target. Curr Drug Targets. 10:372–383. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Ding X, Patel M, Shen D, Herzlich AA, Cao X, Villasmil R, Klupsch K, Tuo J, Downward J and Chan CC: Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci. 50:4957–4966. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Li Q, Hueckstaedt LK and Ren J: The protease inhibitor UCF-101 ameliorates streptozotocin-induced mouse cardiomyocyte contractile dysfunction in vitro: Role of AMP-activated protein kinase. Exp Physiol. 94:984–994. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Zhang C, He A, Liu S, He Q, Luo Y, He Z, Chen Y, Tao A and Yan J: Inhibition of HtrA2 alleviated dextran sulfate sodium (DSS)-induced colitis by preventing necroptosis of intestinal epithelial cells. Cell Death Dis. 10:3442019. View Article : Google Scholar : PubMed/NCBI

9 

Hu Y, Huang M, Wang P, Xu Q and Zhang B: Ucf-101 protects against cerebral oxidative injury and cognitive impairment in septic rat. Int Immunopharmacol. 16:108–113. 2013. View Article : Google Scholar : PubMed/NCBI

10 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Guide for the care and use of laboratory animals (8th edition). National Academies Press (US); Washington, DC: 2011

11 

Kowluru RA and Abbas SN: Diabetes-induced mitochondrial dysfunction in the retina. Invest Ophthalmol Vis Sci. 44:5327–5334. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Qin Z, Yang Y, Wang H, Luo J, Huang X, You J, Wang B and Li M: Role of autophagy and apoptosis in the postinfluenza bacterial pneumonia. Biomed Res Int. 2016:38010262016. View Article : Google Scholar : PubMed/NCBI

13 

Walker CLF, Rudan I, Liu L, Nair H, Theodoratou E, Bhutta ZA, OBrien KL, Campbell H and Black RE: Global burden of childhood pneumonia and diarrhea. Lancet. 381:1405–1416. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Wolf L, Sapich S, Honecker A, Jungnickel C, Seiler F, Bischoff M, Wonnenberg B, Herr C, Schneider-Daum N, Lehr CM, et al: IL-17A-mediated expression of epithelial IL-17C promotes inflammation during acute pseudomonas aeruginosa pneumonia. Am J Physiol Lung Cell Mol Physiol. 311:L1015–L1022. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Wang P, Hu Y, Yao D and Li Y: Omi/HtrA2 regulates a mitochondria- dependent apoptotic pathway in a murine model of septic encephalopathy. Cell Physiol Biochem. 49:2163–2173. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Hussain T, Tan B, Yin Y, Blachier F, Tossou MC and Rahu N: Oxidative stress and inflammation: What polyphenols can do for us? Oxid Med Cell Longev. 2016:74327972016. View Article : Google Scholar : PubMed/NCBI

17 

Floyd RA: Antioxidants, oxidative stress, and degenerative neurological disorders. Proc Soc Exp Biol Med. 222:236–245. 1999. View Article : Google Scholar : PubMed/NCBI

18 

Boskabadi J, Mokhtari-Zaer A, Abareshi A, Khazdair MR, Emami B, Roshan NM, Hosseini M and Boskabady MH: The effect of captopril on lipopolysaccharide-induced lung inflammation. Exp Lung Res. 44:191–200. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Ahmad A, Shameem M and Husain Q: Relation of oxidant-antioxidant imbalance with disease progression in patients with asthma. Ann Thorac Med. 7:226–232. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Tao-Cheng JH: Stimulation-induced structural changes at the nucleus, endoplasmic reticulum and mitochondria of hippocampal neurons. Mol Brain. 11:442018. View Article : Google Scholar : PubMed/NCBI

21 

Zhou D and Jiang Y: Sirtuin 3 attenuates neuroinflammation- induced apoptosis in BV-2 microglia. Aging (Albany NY). 11:9075–9089. 2019. View Article : Google Scholar : PubMed/NCBI

22 

You M, Miao Z, Pan Y and Hu F: Trans-10-hydroxy-2-decenoic acid alleviates LPS-induced blood-brain barrier dysfunction by activating the AMPK/PI3K/AKT pathway. Eur J Pharmacol. 865:1727362019. View Article : Google Scholar : PubMed/NCBI

23 

Luo X, Liu R, Zhang Z, Chen Z, He J and Liu Y: Mitochondrial division inhibitor 1 attenuates mitophagy in a rat model of acute lung injury. Biomed Res Int. 2019:21937062019. View Article : Google Scholar : PubMed/NCBI

24 

Cilenti L, Lee Y, Hess S, Srinivasula S, Park KM, Junqueira D, Davis H, Bonventre JV, Alnemri ES and Zervos AS: Characterization of a novel and specific inhibitor for the pro-apoptotic protease Omi/HtrA2. J Biol Chem. 278:11489–11494. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Saelens X, Festjens N, Walle LV, van Gurp M, van Loo G and Vandenabeele P: Toxic proteins released from mitochondria in cell death. Oncogene. 23:2861–2874. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Brunelle JK and Letai A: Control of mitochondrial apoptosis by the Bcl-2 family. J Cell Sci. 122:437–441. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Lin PY, Tsai CT, Chuang WL, Chao YH, Pan IH, Chen YK, Lin CC and Wang BY: Chlorella sorokiniana induces mitochondrial-mediated apoptosis in human non-small cell lung cancer cells and inhibits xenograft tumor growth in vivo. BMC Complement Altern Med. 17:882017. View Article : Google Scholar : PubMed/NCBI

28 

Krause J, Löser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T and Stenzig J: Rat atrial engineered heart tissue: A new in vitro model to study atrial biology. Basic Res Cardiol. 113:412018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang X: Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats . Mol Med Rep 22: 3127-3134, 2020.
APA
Wang, X. (2020). Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats . Molecular Medicine Reports, 22, 3127-3134. https://doi.org/10.3892/mmr.2020.11410
MLA
Wang, X."Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats ". Molecular Medicine Reports 22.4 (2020): 3127-3134.
Chicago
Wang, X."Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats ". Molecular Medicine Reports 22, no. 4 (2020): 3127-3134. https://doi.org/10.3892/mmr.2020.11410
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X: Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats . Mol Med Rep 22: 3127-3134, 2020.
APA
Wang, X. (2020). Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats . Molecular Medicine Reports, 22, 3127-3134. https://doi.org/10.3892/mmr.2020.11410
MLA
Wang, X."Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats ". Molecular Medicine Reports 22.4 (2020): 3127-3134.
Chicago
Wang, X."Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats ". Molecular Medicine Reports 22, no. 4 (2020): 3127-3134. https://doi.org/10.3892/mmr.2020.11410
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