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Abnormal angiogenesis of placenta in progranulin‑deficient mice

  • Authors:
    • Bairuo Xu
    • Xingyou Chen
    • Yubin Ding
    • Chang Chen
    • Taihang Liu
    • Hua Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, College of Public Health and Health Management, Chongqing Medical University, Chongqing 400016, P.R. China, Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3482-3492
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    Published online on: August 19, 2020
       https://doi.org/10.3892/mmr.2020.11438
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Abstract

Progranulin (PGRN) is a secreted growth factor involved in pleiotropic functions, particularly angiogenesis. A distinctly different placental expression of PGRN has been reported between normal pregnancies and pregnancies with complications, such as pre‑eclampsia or fetal growth restriction. However, the role of PGRN in placental vascular development remains to be elucidated. In the present study, PGRN‑knockout mice (PGRN‑/‑) were used to investigate the role of PGRN in the development of placental blood vessels and placental formation. Placental weights and pup body weights were significantly lower in the PGRN‑/‑ mice compared with the wild‑type mice. Reduced labyrinthine layer areas and aberrant vascularization were also observed via hematoxylin and eosin staining of PGRN‑/‑ mice at embryonic day 14.5 (E14.5) and E17.5. In addition, the morphological data obtained via immunohistochemistry, immunofluorescence staining and western blotting demonstrated decreased expression levels of the blood vessel markers α‑smooth muscle actin and CD31 in PGRN‑/‑ placentas. Furthermore, vasodilator endothelial nitric oxide synthase was reduced in the PGRN‑/‑ placenta. These results indicated that PGRN serves an essential role in the normal angiogenesis of the placental labyrinth in mice.
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Spandidos Publications style
Xu B, Chen X, Ding Y, Chen C, Liu T and Zhang H: Abnormal angiogenesis of placenta in progranulin‑deficient mice. Mol Med Rep 22: 3482-3492, 2020.
APA
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., & Zhang, H. (2020). Abnormal angiogenesis of placenta in progranulin‑deficient mice. Molecular Medicine Reports, 22, 3482-3492. https://doi.org/10.3892/mmr.2020.11438
MLA
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., Zhang, H."Abnormal angiogenesis of placenta in progranulin‑deficient mice". Molecular Medicine Reports 22.4 (2020): 3482-3492.
Chicago
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., Zhang, H."Abnormal angiogenesis of placenta in progranulin‑deficient mice". Molecular Medicine Reports 22, no. 4 (2020): 3482-3492. https://doi.org/10.3892/mmr.2020.11438
Copy and paste a formatted citation
x
Spandidos Publications style
Xu B, Chen X, Ding Y, Chen C, Liu T and Zhang H: Abnormal angiogenesis of placenta in progranulin‑deficient mice. Mol Med Rep 22: 3482-3492, 2020.
APA
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., & Zhang, H. (2020). Abnormal angiogenesis of placenta in progranulin‑deficient mice. Molecular Medicine Reports, 22, 3482-3492. https://doi.org/10.3892/mmr.2020.11438
MLA
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., Zhang, H."Abnormal angiogenesis of placenta in progranulin‑deficient mice". Molecular Medicine Reports 22.4 (2020): 3482-3492.
Chicago
Xu, B., Chen, X., Ding, Y., Chen, C., Liu, T., Zhang, H."Abnormal angiogenesis of placenta in progranulin‑deficient mice". Molecular Medicine Reports 22, no. 4 (2020): 3482-3492. https://doi.org/10.3892/mmr.2020.11438
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