Open Access

Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway

  • Authors:
    • Yu‑Zhu Li
    • Zheng Sun
    • Hong‑Rui Xu
    • Qing‑Gao Zhang
    • Chang‑Qian Zeng
  • View Affiliations

  • Published online on: August 24, 2020     https://doi.org/10.3892/mmr.2020.11455
  • Pages: 3759-3766
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Epilepsy is a syndrome involving chronic recurrent transient brain dysfunction. Activation and proliferation of microglia serve important roles in epilepsy pathogenesis and may be targets for treatment. Although osthole, an active constituent isolated from Cnidium monnieri (L.) Cusson, has been demonstrated to improve epilepsy in rats, its underlying mechanism remains to be elucidated. The present study investigated the effect of osthole on proliferation of kainic acid (KA)‑activated BV‑2 cells and explored the molecular mechanism by which it inhibited their proliferation. Using Cell Counting Kit‑8, enzyme‑linked immunosorbent assay, reverse transcription‑quantitative PCR, western blot analysis and immunofluorescence staining, it was identified that following exposure of KA‑activated BV‑2 cells to 131.2 µM osthole for 24 h, cell proliferation and release of tumor necrosis factor α, interleukin 6 and nitric oxide synthase/induced nitric oxide synthase were significantly inhibited (P<0.05). Further experiments revealed that osthole significantly downregulated mRNA and protein levels of Notch signaling components in KA‑activated BV‑2 cells (P<0.05). Therefore, it was hypothesized that osthole inhibited the proliferation of microglia by modulating the Notch signaling pathway, which may be useful for the treatment of epilepsy and other neurodegenerative diseases characterized by Notch upregulation.
View Figures
View References

Related Articles

Journal Cover

November-2020
Volume 22 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li YZ, Sun Z, Xu HR, Zhang QG and Zeng CQ: Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Mol Med Rep 22: 3759-3766, 2020
APA
Li, Y., Sun, Z., Xu, H., Zhang, Q., & Zeng, C. (2020). Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Molecular Medicine Reports, 22, 3759-3766. https://doi.org/10.3892/mmr.2020.11455
MLA
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22.5 (2020): 3759-3766.
Chicago
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22, no. 5 (2020): 3759-3766. https://doi.org/10.3892/mmr.2020.11455