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Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway

  • Authors:
    • Yu‑Zhu Li
    • Zheng Sun
    • Hong‑Rui Xu
    • Qing‑Gao Zhang
    • Chang‑Qian Zeng
  • View Affiliations / Copyright

    Affiliations: Department of Medical College, Dalian University, Dalian, Liaoning 116622, P.R. China, Beijing International Travel Health Care Center of Beijing Entry‑Exit Inspection and Quarantine Bureau, Beijing 100088, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3759-3766
    |
    Published online on: August 24, 2020
       https://doi.org/10.3892/mmr.2020.11455
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Abstract

Epilepsy is a syndrome involving chronic recurrent transient brain dysfunction. Activation and proliferation of microglia serve important roles in epilepsy pathogenesis and may be targets for treatment. Although osthole, an active constituent isolated from Cnidium monnieri (L.) Cusson, has been demonstrated to improve epilepsy in rats, its underlying mechanism remains to be elucidated. The present study investigated the effect of osthole on proliferation of kainic acid (KA)‑activated BV‑2 cells and explored the molecular mechanism by which it inhibited their proliferation. Using Cell Counting Kit‑8, enzyme‑linked immunosorbent assay, reverse transcription‑quantitative PCR, western blot analysis and immunofluorescence staining, it was identified that following exposure of KA‑activated BV‑2 cells to 131.2 µM osthole for 24 h, cell proliferation and release of tumor necrosis factor α, interleukin 6 and nitric oxide synthase/induced nitric oxide synthase were significantly inhibited (P<0.05). Further experiments revealed that osthole significantly downregulated mRNA and protein levels of Notch signaling components in KA‑activated BV‑2 cells (P<0.05). Therefore, it was hypothesized that osthole inhibited the proliferation of microglia by modulating the Notch signaling pathway, which may be useful for the treatment of epilepsy and other neurodegenerative diseases characterized by Notch upregulation.
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Copy and paste a formatted citation
Spandidos Publications style
Li YZ, Sun Z, Xu HR, Zhang QG and Zeng CQ: Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Mol Med Rep 22: 3759-3766, 2020.
APA
Li, Y., Sun, Z., Xu, H., Zhang, Q., & Zeng, C. (2020). Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Molecular Medicine Reports, 22, 3759-3766. https://doi.org/10.3892/mmr.2020.11455
MLA
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22.5 (2020): 3759-3766.
Chicago
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22, no. 5 (2020): 3759-3766. https://doi.org/10.3892/mmr.2020.11455
Copy and paste a formatted citation
x
Spandidos Publications style
Li YZ, Sun Z, Xu HR, Zhang QG and Zeng CQ: Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Mol Med Rep 22: 3759-3766, 2020.
APA
Li, Y., Sun, Z., Xu, H., Zhang, Q., & Zeng, C. (2020). Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway. Molecular Medicine Reports, 22, 3759-3766. https://doi.org/10.3892/mmr.2020.11455
MLA
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22.5 (2020): 3759-3766.
Chicago
Li, Y., Sun, Z., Xu, H., Zhang, Q., Zeng, C."Osthole inhibits proliferation of kainic acid‑activated BV‑2 cells by modulating the Notch signaling pathway". Molecular Medicine Reports 22, no. 5 (2020): 3759-3766. https://doi.org/10.3892/mmr.2020.11455
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