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Article Open Access

Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts

  • Authors:
    • Changqing Xu
    • Xuqing Huang
    • Yueyang Tong
    • Xiaocheng Feng
    • Yan Wang
    • Cancan Wang
    • Yuyue Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Respiration, The Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang 310015, P.R. China, Department of Endocrinology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310016, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3833-3839
    |
    Published online on: August 24, 2020
       https://doi.org/10.3892/mmr.2020.11458
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Abstract

Icariin (ICA) has been used as a promising anti‑aging drug; however, its underlying molecular mechanism is yet to be elucidated. The present study aimed to determine the anti‑aging molecular mechanisms of ICA. D‑galactose (D‑gal) was used to generate a cell aging model. IMR‑90 human lung fibroblasts were pretreated with different concentrations of ICA (1, 2, 4, 8 and 16 µmol/l) for 6 h and subsequently incubated with D‑gal (200 mmol/l) at 37˚C for 72 h. Senescence of IMR‑90 cells was assessed by senescence‑associated‑β‑galactosidase (SA‑β‑Gal) staining assay. Cell viability, and the expression levels of p53/p21, sirtuin (SIRT) 1/6 and p50/p65 were determined via the MTT assay and western blotting respectively. The results demonstrated that D‑gal notably increased the proportion of SA‑β‑Gal‑positive cells and decreased the viability of IMR‑90 cells; however, pretreatment with ICA reversed the effects of D‑gal on IMR‑90 cells in a concentration‑dependent manner. Furthermore, it was also demonstrated that the activation of p53/p21 and nuclear factor‑κB (NF‑κB) signaling, and downregulation of SIRT1/6 may be involved in IMR‑90 cells, in D‑gal‑induced aging and ICA may effectively prevent IMR‑90 cells from these changes induced by D‑gal. Taken together, the results of the present study suggest that the anti‑aging molecular mechanisms of ICA may be associated with the regulation of the SIRT1/NF‑κB pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Xu C, Huang X, Tong Y, Feng X, Wang Y, Wang C and Jiang Y: Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts. Mol Med Rep 22: 3833-3839, 2020.
APA
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., & Jiang, Y. (2020). Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts. Molecular Medicine Reports, 22, 3833-3839. https://doi.org/10.3892/mmr.2020.11458
MLA
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., Jiang, Y."Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts". Molecular Medicine Reports 22.5 (2020): 3833-3839.
Chicago
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., Jiang, Y."Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts". Molecular Medicine Reports 22, no. 5 (2020): 3833-3839. https://doi.org/10.3892/mmr.2020.11458
Copy and paste a formatted citation
x
Spandidos Publications style
Xu C, Huang X, Tong Y, Feng X, Wang Y, Wang C and Jiang Y: Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts. Mol Med Rep 22: 3833-3839, 2020.
APA
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., & Jiang, Y. (2020). Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts. Molecular Medicine Reports, 22, 3833-3839. https://doi.org/10.3892/mmr.2020.11458
MLA
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., Jiang, Y."Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts". Molecular Medicine Reports 22.5 (2020): 3833-3839.
Chicago
Xu, C., Huang, X., Tong, Y., Feng, X., Wang, Y., Wang, C., Jiang, Y."Icariin modulates the sirtuin/NF‑κB pathway and exerts anti‑aging effects in human lung fibroblasts". Molecular Medicine Reports 22, no. 5 (2020): 3833-3839. https://doi.org/10.3892/mmr.2020.11458
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