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Differential expression and inhibitory effects of aquaporins on the development of adenomyosis

  • Authors:
    • Liping Jia
    • Yuzhu Liu
    • Yixu Han
    • Xiaofei Zhou
    • Fahui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, The Second Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3840-3850
    |
    Published online on: September 2, 2020
       https://doi.org/10.3892/mmr.2020.11479
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Abstract

Aquaporin 2 (AQP2), AQP5 and AQP8 participate in adenomyosis (AM). Ηowever, the roles of these three molecules in AM have not been fully elucidated. In the present study, Institute of Cancer Research female mice were used to establish a model of AM. Subsequently, the endometrial tissues of the mice were observed by hematoxylin‑eosin staining, and AM severity, uterus diameter, uterus index, ovary index and numbers of nodules on the uterine surface were evaluated and counted. In addition, eutopic and ectopic endometrial stromal cells (ESCs) were isolated from eutopic and ectopic endometrial samples derived from patients with AM and were then identified by immunofluorescence. The viability, and migratory and invasive ability of ESCs transfected with small interfering RNA targeting AQP5 (siAQP5) were determined by Cell Counting Κit‑8, scratch wound‑healing and Transwell assays, respectively. Reverse transcription‑quantitative polymerase chain reaction was performed to determine the mRNA expression levels of AQP5, epithelial‑mesenchymal transition (EMT)‑related genes (E‑and N‑cadherin), matrix metalloproteinase (MMP)‑2 and ‑9. Protein expression levels of AQP2, AQP5, AQP8, E‑, N‑cadherin, MMP‑2 and ‑9 were detected by western blotting. AM severity and uterus index were higher, and there were a greater number of nodules on the uterine surface in the AM group compared with the sham group. AQP2, AQP5 and AQP8 proteins were highly expressed in eutopic and ectopic endometrium of the AM group, and AQP5 was more highly expressed than AQP2 or AQP8. In addition, the data showed that Vimentin was positively expressed in ESCs, and that siAQP5 suppressed the mRNA expression levels of AQP5, cell viability, migration, invasion, EMT and MMP‑2 and ‑9 expression in ESCs. In conclusion, AQP2, AQP5 and AQP8 were highly expressed in eutopic and ectopic endometrium. Notably, AQP5 silencing may suppress AM by inhibiting viability, migration, invasion, EMT, and MMP‑2 and ‑9 expression in ESCs.
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1 

Struble J, Reid S and Bedaiwy MA: Adenomyosis: A clinical review of a challenging gynecologic condition. J Minim Invasive Gynecol. 23:164–185. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Harada T, Khine YM, Kaponis A, Nikellis T, Decavalas G and Taniguchi F: The lity. Obstet Gynecol Surv. 71:557–568. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Vlahos NF, Theodoridis TD and Partsinevelos GA: Myomas and adenomyosis: Impact on reproductive outcome. Biomed Res Int. 2017:59264702017. View Article : Google Scholar : PubMed/NCBI

4 

Pontis A, D'Alterio MN, Pirarba S, de Angelis C, Tinelli R and Angioni S: Adenomyosis: A systematic review of medical treatment. Gynecol Endocrinol. 32:696–700. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Li C and Wang W: Molecular biology of aquaporins. Adv Exp Med Biol. 969:1–34. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Soveral G and Casini A: Aquaporin modulators: A patent review (2010–2015). Expert Opin Ther Pat. 27:49–62. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Beitz E, Golldack A, Rothert M and von Bulow J: Challenges and achievements in the therapeutic modulation of aquaporin functionality. Pharmacol Ther. 155:22–35. 2015. View Article : Google Scholar : PubMed/NCBI

8 

He Y, Zhou L, Fan Z, Liu S and Fang W: Palmitic acid, but not high-glucose, induced myocardial apoptosis is alleviated by N-acetylcysteine due to attenuated mitochondrial-derived ROS accumulation-induced endoplasmic reticulum stress. Cell Death Dis. 9:5682018. View Article : Google Scholar : PubMed/NCBI

9 

García-Solares J, Donnez J, Donnez O and Dolmans MM: Pathogenesis of uterine adenomyosis: Invagination or metaplasia? Fertil Steril. 109:371–379. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Benagiano G, Brosens I and Habiba M: Structural and molecular features of the endomyometrium in endometriosis and adenomyosis. Hum Reprod Update. 20:386–402. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Zhou YF, Mori T, Nagasawa H, Nakayama T, Kubota T and Sakamoto S: Probucol, a hypocholesterolemic agent, prevents the development of uterine adenomyosis induced by pituitary grafting in mice. Anticancer Res. 24:2209–2212. 2004.PubMed/NCBI

12 

Jiang XX, Fei XW, Zhao L, Ye XL, Xin LB, Qu Y, Xu KH, Wu RJ and Lin J: Aquaporin 5 plays a role in estrogen-induced ectopic implantation of endometrial stromal cells in endometriosis. PLoS One. 10:e01452902015. View Article : Google Scholar : PubMed/NCBI

13 

Xin LB, Jiang XX, Ye XL, Wu RJ, Xu KH, Ma JY and Lin J: AQP5 gene silencing inhibits proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis. Zhejiang Da Xue Xue Bao Yi Xue. 44:285–292. 2015.(In Chinese).

14 

National Research Council Institute for Laboratory Animal Research. Guide for the Care and Use of Laboratory Animals. National Academies Press (US) Copyright 1996 by the National Academy of Sciences. All rights reserved.; Washington (DC): 1996

15 

Koujyo T, Hatakeyama S, Yamada H, Iwabuchi K, Kajino K, Ogasawara K, Onoe K and Fujimoto S: Induction of endometriosis and adenomyosis by transvaginal pituitary transplantation in mice with and without natural killer cell activity. Am J Reprod Immunol. 40:441–446. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Mori T, Kyokuwa M and Nagasawa H: Animal model of uterine adenomyosis: Induction of the lesion in rats by ectopic pituitary isografting. Lab Anim Sci. 48:64–68. 1998.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression using different real-time quantitative PCR and 2(-Delta Delta C(T)) method. Methods. 402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Li Q, Yang T, Li D, Ding F, Bai G, Wang W and Sun H: Knockdown of aquaporin-5 sensitizes colorectal cancer cells to 5-fluorouracil via inhibition of the Wnt-β-catenin signaling pathway. Biochem Cell Biol. 96:572–579. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Cheong ML, Lai TH and Wu WB: Connective tissue growth factor mediates transforming growth factor β-induced collagen expression in human endometrial stromal cells. PLoS One. 14:e02107652019. View Article : Google Scholar : PubMed/NCBI

20 

Sundqvist J, Andersson KL, Scarselli G, Gemzell-Danielsson K and Lalitkumar PG: Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: A link to the aetiology of endometriosis. Hum Reprod. 27:2737–2746. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Yamin Hu and Bing ZH: Expression of VEGF-C and ICAM-1 in adenomyosis and clinical significance. Acta Med Univ Sci Technol Huazhong. 38:217–219. 2009.

22 

Huang YH, Zhou XY, Wang HM, Xu H, Chen J and Lv NH: Aquaporin 5 promotes the proliferation and migration of human gastric carcinoma cells. Tumour Biol. 34:1743–1751. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Wang S, Zhao X, Yang S, Chen B and Shi J: Salidroside alleviates high glucose-induced oxidative stress and extracellular matrix accumulation in rat glomerular mesangial cells by the TXNIP-NLRP3 inflammasome pathway. Chem Biol Interact. 278:48–53. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Li J, Wang Z, Chong T, Chen H, Li H, Li G, Zhai X and Li Y: Over-expression of a poor prognostic marker in prostate cancer: AQP5 promotes cells growth and local invasion. World J Surg Oncol. 12:2842014. View Article : Google Scholar : PubMed/NCBI

25 

Jiang XX, Xu KH, Ma JY, Tian YH, Guo XY, Lin J and Wu RJ: Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5. Oncol Lett. 4:257–261. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Chae YK, Woo J, Kim MJ, Kang SK, Kim MS, Lee J, Lee SK, Gong G, Kim YH, Soria JC, et al: Expression of aquaporin 5 (AQP5) promotes tumor invasion in human non small cell lung cancer. PLoS One. 3:e21622008. View Article : Google Scholar : PubMed/NCBI

27 

Chen Q, Jin M, Yang F, Zhu J, Xiao Q and Zhang L: Matrix metalloproteinases: Inflammatory regulators of cell behaviors in vascular formation and remodeling. Mediators Inflamm. 2013:9283152013. View Article : Google Scholar : PubMed/NCBI

28 

Webb AH, Gao BT, Goldsmith ZK, Irvine AS, Saleh N, Lee RP, Lendermon JB, Bheemreddy R, Zhang Q, Brennan RC, et al: Inhibition of MMP-2 and MMP-9 decreases cellular migration, and angiogenesis in in vitro models of retinoblastoma. BMC Cancer. 17:4342017. View Article : Google Scholar : PubMed/NCBI

29 

Yi KW, Kim SH, Ihm HJ, Oh YS, Chae HD, Kim CH and Kang BM: Increased expression of p21-activated kinase 4 in adenomyosis and its regulation of matrix metalloproteinase-2 and −9 in endometrial cells. Fertil Steril. 103:1089–1097.e2. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Li T, Li YG and Pu DM: Matrix metalloproteinase-2 and −9 expression correlated with angiogenesis in human adenomyosis. Gynecol Obstet Invest. 62:229–235. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Tulchinsky E, Demidov O, Kriajevska M, Barlev NA and Imyanitov E: EMT: A mechanism for escape from EGFR-targeted therapy in lung cancer. Biochim Biophys Acta Rev Cancer. 1871:29–39. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Jakobsen KR, Demuth C, Sorensen BS and Nielsen AL: The role of epithelial to mesenchymal transition in resistance to epidermal growth factor receptor tyrosine kinase inhibitors in non-small cell lung cancer. Transl Lung Cancer Res. 5:172–182. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Ramamurthy VP, Ramalingam S, Gediya LK and Njar VCO: The retinamide VNLG-152 inhibits f-AR/AR-V7 and MNK-eIF4E signaling pathways to suppress EMT and castration-resistant prostate cancer xenograft growth. FEBS J. 285:1051–1063. 2018. View Article : Google Scholar : PubMed/NCBI

34 

He Z, Dong W, Hu J and Ren X: AQP5 promotes hepatocellular carcinoma metastasis via NF-κB-regulated epithelial-mesenchymal transition. Biochem Biophys Res Commun. 490:343–348. 2017. View Article : Google Scholar : PubMed/NCBI

35 

Wang W, Li Q, Yang T, Li D, Ding F, Sun H and Bai G: RNA interference-mediated silencing of aquaporin (AQP)-5 hinders angiogenesis of colorectal tumor by suppressing the production of vascular endothelial growth factor. Neoplasma. 65:55–65. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jia L, Liu Y, Han Y, Zhou X and Wang F: Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Mol Med Rep 22: 3840-3850, 2020.
APA
Jia, L., Liu, Y., Han, Y., Zhou, X., & Wang, F. (2020). Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Molecular Medicine Reports, 22, 3840-3850. https://doi.org/10.3892/mmr.2020.11479
MLA
Jia, L., Liu, Y., Han, Y., Zhou, X., Wang, F."Differential expression and inhibitory effects of aquaporins on the development of adenomyosis". Molecular Medicine Reports 22.5 (2020): 3840-3850.
Chicago
Jia, L., Liu, Y., Han, Y., Zhou, X., Wang, F."Differential expression and inhibitory effects of aquaporins on the development of adenomyosis". Molecular Medicine Reports 22, no. 5 (2020): 3840-3850. https://doi.org/10.3892/mmr.2020.11479
Copy and paste a formatted citation
x
Spandidos Publications style
Jia L, Liu Y, Han Y, Zhou X and Wang F: Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Mol Med Rep 22: 3840-3850, 2020.
APA
Jia, L., Liu, Y., Han, Y., Zhou, X., & Wang, F. (2020). Differential expression and inhibitory effects of aquaporins on the development of adenomyosis. Molecular Medicine Reports, 22, 3840-3850. https://doi.org/10.3892/mmr.2020.11479
MLA
Jia, L., Liu, Y., Han, Y., Zhou, X., Wang, F."Differential expression and inhibitory effects of aquaporins on the development of adenomyosis". Molecular Medicine Reports 22.5 (2020): 3840-3850.
Chicago
Jia, L., Liu, Y., Han, Y., Zhou, X., Wang, F."Differential expression and inhibitory effects of aquaporins on the development of adenomyosis". Molecular Medicine Reports 22, no. 5 (2020): 3840-3850. https://doi.org/10.3892/mmr.2020.11479
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